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Fluorescent acrylamide nanoparticles for boronic acid based sugar sensing — from probes to sensors

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Abstract

Fluorescent nanoparticles containing covalently bound phenylboronic acids (~ 250 nm in diameter) are presented that respond to carbohydrates by swelling which is detected using fluorescence resonance energy transfer. The nanoparticles are characterized in terms of kinetics, response time and dynamic range. The response of the particles to glucose at pH 7.5 depends on the kind of phenylboronic acid and on ionic strength. The particles were immobilized in hydrogel sensor layers that enable continuous optical sensing of carbohydrates.

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Acknowledgements

Klaus Koren and the FELMI-ZMF institute are gratefully thanked for preparing and taking the SEM pictures. Herbert Motter is thanked for the digestion and the measurements on the ICP-OES.

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Authors and Affiliations

  1. Graz University of Technology, Institute of Analytical Chemistry and Radiochemistry, Stremayrgasse 16/III, A-8010, Graz, Austria

    Gunter Zenkl & Ingo Klimant

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  1. Gunter Zenkl
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  2. Ingo Klimant
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Correspondence to Gunter Zenkl.

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Supporting Information

Materials, synthesis of the monomers, measurement methods and additional figures can be found in the Supporting Information of the on-line version of this article. (PDF 858 kb)

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Zenkl, G., Klimant, I. Fluorescent acrylamide nanoparticles for boronic acid based sugar sensing — from probes to sensors. Microchim Acta 166, 123–131 (2009). https://doi.org/10.1007/s00604-009-0172-0

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  • DOI: https://doi.org/10.1007/s00604-009-0172-0

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