Microchimica Acta

, Volume 166, Issue 1–2, pp 35–39 | Cite as

A micro-electrophoresis system based on a short capillary with hydrostatic pressure assisted separation and injection

Original Paper
First Online:
Received:
Accepted:

Abstract

A simple micro-capillary electrophoresis system to be used as disposable device was developed. A short commercial capillary was used as the separation channel, hydrostatic pressure generated by the sample employed for injection, and a voltage of 200 V used for separation in a 6 cm long capillary assisted by hydrostatic pressure of the carrier. The device was used for the separation of dopamine and catechol. Good reproducibility and efficiency was obtained. Because the instrumentation and operation conditions were simplified, and a replaceable modular separation channel was used, the proposed micro-capillary electrophoresis system is potentially useful in disposable devices.

Keywords

Micro-capillary electrophoresis Disposable device Hydrostatic pressure Pressure assisted separation 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (NSFC) No. 20875080 and Jiangsu Environmental Protection Project 2007025.

References

  1. 1.
    Manz A, Grabner N, Widmer HM (1990) Miniaturized total chemical analysis systems: a novel concept for chemical sensing. Sens Actuators B 1:244CrossRefGoogle Scholar
  2. 2.
    Janasek D, Franzke J, Manz A (2006) Scaling and the design of miniaturized chemical-analysis systems. Nature 442:374CrossRefGoogle Scholar
  3. 3.
    Fredrickson CK, Fan ZH (2004) Macro-to-micro interfaces for microfluidic devices. Lab Chip 4:526CrossRefGoogle Scholar
  4. 4.
    Scampicchio M, Ballabio D, Arecchi A, Cosio SM, Mannino S (2008) Amperometric electronic tongue for food analysis. Microchim Acta 163:11CrossRefGoogle Scholar
  5. 5.
    Du WB, Fang Q, Fang ZL (2006) Microfluidic sequential injection analysis in a short capillary. Anal Chem 78:6404CrossRefGoogle Scholar
  6. 6.
    Matysik FM (2008) Advances in amperometric and conductometric detection in capillary and chip-based electrophoresis. Microchim Acta 160:1CrossRefGoogle Scholar
  7. 7.
    Chen G, Guo JJ, Xu XJ (2007) Fabrication of a fiberglass-packed channel in a microchip for flow injection analysis. Microchim Acta 159:191CrossRefGoogle Scholar
  8. 8.
    Whitesides GM (2006) The origins and the future of microfluidics. Nature 442:368CrossRefGoogle Scholar
  9. 9.
    Alarie JP, Jacobson SC, Ramsey JM (2001) Electrophoretic injection bias in a microchip valving scheme. Electrophoresis 22:312CrossRefGoogle Scholar
  10. 10.
    Slentz BE, Penner NA, Regnier F (2002) Sampling BIAS at channel junctions in gated flow injection on chips. Anal Chem 74:4835CrossRefGoogle Scholar
  11. 11.
    Zhang L, Yin X, Fang Z (2006) Negative pressure pinched sample injection for microchip-based electrophoresis. Lab Chip 6:258CrossRefGoogle Scholar
  12. 12.
    Gai H, Yu L, Dai Z, Ma Y, Lin BC (2004) Injection by hydrostatic pressure in conjunction with electrokinetic force on a microfluidic chip. Electrophoresis 25:1888CrossRefGoogle Scholar
  13. 13.
    Luo Y, Wu D, Zeng S, Gai H, Long Z, Shen Z, Dai Z, Qin J, Lin BC (2006) Double-cross hydrostatic pressure sample injection for chip CE: Variable sample plug volume and minimum number of electrodes. Anal Chem 78:6074CrossRefGoogle Scholar
  14. 14.
    Wang W, Zhou F, Zhao L, Zhang JR, Zhu JJ (2008) Improved hydrostatic pressure sample injection by tilting the microchip towards the disposable miniaturized CE device. Electrophoresis 29:561CrossRefGoogle Scholar
  15. 15.
    Linhares MC, Kissinger PT (1991) Use of an on-column fracture in capillary zone electrophoresis for sample introduction. Anal Chem 63:2076CrossRefGoogle Scholar
  16. 16.
    Wei H, Ang KC, Li SFY (1998) Minimization of sample discrimination introduced by on-column fracture electrokinetic injection in capillary electrophoresis. Anal Chem 70:2248CrossRefGoogle Scholar
  17. 17.
    Zhai C, Li C, Qiang W, Lei J, Yu X, Ju H (2007) Amperometric Detection of Carbohydrates with a Portable Silicone/Quartz Capillary Microchip by Designed Fracture Sampling. Anal Chem 79:9427CrossRefGoogle Scholar
  18. 18.
    Xu JJ, Bao N, Xia XH, Peng Y, Chen HY (2004) Electrochemical detection method for nonelectroactive and electroactive analytes in microchip electrophoresis. Anal Chem 76:6902CrossRefGoogle Scholar
  19. 19.
    Adamson AW, Gast AP (1997) Physical Chemistry of Surfaces, 6th edn. John Wiley & Sons, New York, p 6Google Scholar
  20. 20.
    Lide DR (2004) CRC Handbook of Chemistry and Physics, 84th edn. CRC Press, Boca Raton, FLGoogle Scholar
  21. 21.
    Nakayama Y, Boucher RF (1999) Introduction to Fluid Mechanics. Arnold, LondonGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.School of Chemical and Biological EngineeringYancheng Institute of TechnologyYanchengPeople’s Republic of China
  2. 2.Key Lab of Analytical Chemistry for Life Science, School of Chemistry & Chemical EngineeringNanjing UniversityNanjingChina

Personalised recommendations