Microchimica Acta

, 165:299 | Cite as

Determination of hydrogen peroxide with the aid of peroxidase-like Fe3O4 magnetic nanoparticles as the catalyst

  • Qing Chang
  • Kejian Deng
  • Lihua ZhuEmail author
  • Guodong Jiang
  • Chen Yu
  • Heqing TangEmail author
Original Paper


As a mimetic peroxidase, Fe3O4 magnetic nanoparticles (MNPs) were prepared and used for the determination of hydrogen peroxide (H2O2) based on their catalytic effect on the oxidation of N,N-diethyl-p-phenylenediamine sulfate (DPD). Fe3O4 MNPs were found to be able to activate H2O2 and oxidize DPD to a colored product with a strong absorption maximum at 550 nm. Under optimized conditions, the absorbance of the product responded linearly to H2O2 concentration in the range from 0.5 to 150 × 10−6 mol L−1 H2O2 with a detection limit as low as 2.5 × 10−7 mol L−1. The method was successfully applied to the determination of H2O2 in rainwater, honey and milk samples.


Fe3O4 magnetic nanoparticles Peroxidase-like Hydrogen peroxide Catalytic oxidation Spectrophotometry 



This work was partially supports by grant from the National Science Foundation of China (grants Nos. 20877031 and 20677019) and from Hubei Key Laboratory for Catalysis and Material Science in South-Central University for Nationalities.

Supplementary material

ESM 1 (DOC 43.0 KB).
ESM 2 (DOC 44.5 KB).
ESM 3 (DOC 39.0 KB).


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhanPeoples Republic of China
  2. 2.Hubei Key Laboratory for Catalysis and Material Science, College of Chemistry and Material ScienceSouth-Central University for NationalitiesWuhanPeople’s Republic of China

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