Microchimica Acta

, Volume 164, Issue 1–2, pp 177–183 | Cite as

Amperometric biosensors for lactic acid based on baker’s and wine yeast

  • Rasa Garjonyte
  • Vytautas Melvydas
  • Albertas Malinauskas
Original Paper
First Online:
Received:
Accepted:

Abstract

Electrodes modified with a layer of baker’s and wine yeast Saccharomyces cerevisiae (dried at various temperatures) were investigated as amperometric biosensors for lactic acid in the presence of mediators such as potassium ferricyanide, phenazine methosulphate, 1,2-naphthoquinone-4-sulfonic acid sodium salt and p-benzoquinone. Potassium ferricyanide was found to be the most suitable mediator in terms of electrode sensitivity and stability. The linear range of the current responses to the concentration of lactic acid was up to 1 mM. The relationship of electrode responses to lactic acid and viability of yeast cells was investigated.

Keywords

Biosensor Saccharomyces cerevisiae Flavocytochrome b2 Lactic acid Mediator 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgement

The support from the Lithuanian State Science and Studies Foundation (project No. C-03047) is gratefully acknowledged.

References

  1. 1.
    Nicolaus N, Strehlitz B (2008) Amperometric lactate biosensors and their application in (sports) medicine, for life quality and wellbeing. Microchim Acta 160:15CrossRefGoogle Scholar
  2. 2.
    Gerard M, Ramanathan K, Chaubey A, Malhotra BD (1996) Immobilization of lactate dehydrogenase on electrochemically prepared polyaniline films. Electroanal 11:450CrossRefGoogle Scholar
  3. 3.
    Chaubey A, Gerard M, Singhal R, Singh VS, Malhotra BD (2000) Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole-polyvinylsulfonate composite films for application to lactate biosensors. Electrochim Acta 46:723CrossRefGoogle Scholar
  4. 4.
    Zayats M, Kharitonov AB, Katz E, Buckmann AF, Wilner I (2000) An integrated NAD+-dependent enzyme-functionalized field effect transistor (ENFE+) system: development of a lactate biosensor. Biosens Bioelectron 15:671CrossRefGoogle Scholar
  5. 5.
    Leonida MD, Starczynowski DT, Waldman R, Aurian-Blajeni B (2003) Polymeric FAD used as enzyme-friendly mediator in lactate detection. Anal Bioanal Chem 376:832CrossRefGoogle Scholar
  6. 6.
    Spohn U, Narasaiah D, Gorton L (1996) The influence of the carbon paste composition on the performance of an amperometric bienzyme sensor for l-lactate. Electroanal 8:567Google Scholar
  7. 7.
    Garjonyte R, Yigzaw Y, Meskys R, Malinauskas A, Gorton L (2001) Prussian Blue- and lactate oxidase-based biosensor for lactic acid. Sens Act B 87:33CrossRefGoogle Scholar
  8. 8.
    Hirano K, Yamato H, Kurimoto K, Ohwa M (2002) Design of novel electron transfer mediators based on indophenol derivatives for lactate biosensor. Biosens Bioelectron 17:315CrossRefGoogle Scholar
  9. 9.
    Kulys JJ, Svirmickas GJS (1980) Reagentless lactate sensor based on cytochrome b 2. Anal Chim Acta 117:115CrossRefGoogle Scholar
  10. 10.
    Staskeviciene SL, Cenas NK, Kulys JJ (1991) Reagentless lactate electrodes based on electrocatalytic oxidation of flavocytochrome b 2. Anal Chim Acta 243:167CrossRefGoogle Scholar
  11. 11.
    Amine A, Deni J, Kaufmann JM (1994) Amperometric biosensor based on carbon paste mixed with enzyme, lipid and cytochrome c. Bioelectrochem Bioenerg 34:123CrossRefGoogle Scholar
  12. 12.
    Smutok O, Gayda G, Gonchar M, Schuhmann W (2005) A novel l-lactate selective biosensor based on flavocytochrome b 2 from methylotropic yeast Hansenula polymorpha. Biosens Bioelectron 20:1285CrossRefGoogle Scholar
  13. 13.
    Kulys J, Wang L, Razumas V (1992) Sensitive yeast bioelectrode to l-lactate. Electroanal 4:527CrossRefGoogle Scholar
  14. 14.
    Garjonyte R, Malinauskas A (2003) Investigation of baker’s yeast Saccharomyces cerevisiae- and mediator-based carbon paste electrodes as amperometric biosensors for lactic acid. Sens Act B 96:509CrossRefGoogle Scholar
  15. 15.
    Garjonyte R, Melvydas V, Malinauskas A (2006) Mediated amperometric biosensors for lactic acid based on carbon paste electrodes modified with baker’s yeast Saccharomyces cerevisiae. Bioelectrochem 68:191CrossRefGoogle Scholar
  16. 16.
    D’Souza SF (2001) Microbial biosensors. Biosens Bioelectron 16:337CrossRefGoogle Scholar
  17. 17.
    Baronian KHR (2004) The use of yeasts and moulds as sensing elements in biosensors. Biosens Bioelectron 19:953CrossRefGoogle Scholar
  18. 18.
    Khlupova M, Kuznetsov B, Demkiv O, Gonchar M, Csoregi E, Shleev S (2007) Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde. Talanta 71:934CrossRefGoogle Scholar
  19. 19.
    Khlupova M, Kuznetsov B, Gonchar M, Ruzgas T, Shleev S (2007) Amperometric monitoring of redox activity in intact, permeabilised and lyophilised cells of the yeast Hansenula polymorpha. Electrochem Commun 9:1480CrossRefGoogle Scholar
  20. 20.
    Baronian KHR, Downard AJ, Lowen RK, Pasco N (2002) Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method. Appl Microbiol Technol 60:108CrossRefGoogle Scholar
  21. 21.
    Heiskanen A, Yakovleva J, Spegel C, Taboryski R, Koudelka-Hep M, Emneus J, Ruzgas T (2004) Amperometric monitoring of redox activity in living cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators. Electrochem Commun 6:219CrossRefGoogle Scholar
  22. 22.
    Zhao J, Wang M, Yang Z, Gong Q, Lu Y, Yang Z (2005) Mediated electrochemical measurement of inhibitory effects of furfural and acetic acid on Saccharomyces cerevisiae and Candida shehatae”, Biotechnol. Lett 27:207Google Scholar
  23. 23.
    Wang M, Zhao J, Yang Z, Du Z, Yang Z (2007) Electrochemical insights into the ethanol tolerance of Saccharomyces cerevisiae. Bioelectrochem 71:107CrossRefGoogle Scholar
  24. 24.
    Daff S, Ingledew WJ, Reid GA, Chapman SK (1996) New insights into the catalytic cycle of flavocytochrome b 2. Biochemistry 35:6345CrossRefGoogle Scholar
  25. 25.
    Smutok OV, Os’mak GS, Gaida GZ, Gonchar MV (2006) Screening of yeasts producing stable l-lactate cytochrome c oxireductase and study of the regulation of enzyme synthesis. Microbiology 75:20CrossRefGoogle Scholar
  26. 26.
    Urban P, Lederer F (1988) Inactivation of flavocytochrome b 2 with fluoropyruvate; reaction at the active site histidine. Eur J Biochem 173:155CrossRefGoogle Scholar
  27. 27.
    Marchesiello M, Genies EM (1992) Glucose sensor: polypyrrole-glucose oxidase electrode in the presence of p-benzoquinone. Electrochim. Acta 37:1987CrossRefGoogle Scholar
  28. 28.
    Ng LT, Yuan YH, Zhao H (1998) Natural polymer-based sulfite biosensor. Electroanal 10:1119CrossRefGoogle Scholar
  29. 29.
    Takayama T, Kurosaki T, Ikeda T (1993) Mediated electrocatalysis at a biocatalyst electrode based on a bacterium Gluconobacter industrius. J Electroanal Chem 356:295CrossRefGoogle Scholar
  30. 30.
    Takayama K, Ikeda T, Nagasawa T (1996) Mediated amperometric biosensor for nicotinic acid based on whole cells of Pseudomonas fluorescens. Electroanal 8:765CrossRefGoogle Scholar
  31. 31.
    Lorenzen PC, Ebert Y, Clawin-Radecker I, Sclimme E (2003) Influence of heat impact in reconstituted skim milk on the properties of yoghurt fermented by ropy or non-ropy starter culture. Nahr Food 47:349CrossRefGoogle Scholar
  32. 32.
    Garmiene G, Salkauskiene V, Kulikauskiene M (2005) Lactic acid isomers in milk products. Milchwissenschaft 60:259Google Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Rasa Garjonyte
    • 1
  • Vytautas Melvydas
    • 2
  • Albertas Malinauskas
    • 1
  1. 1.Institute of ChemistryVilniusLithuania
  2. 2.Institute of BotanyVilniusLithuania

Personalised recommendations