Microchimica Acta

, Volume 164, Issue 1–2, pp 167–171 | Cite as

A reagentless nitrite biosensor based on direct electron transfer of hemoglobin on a room temperature ionic liquid/carbon nanotube-modified electrode

Original Paper
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Accepted:

Abstract

A novel amperometric biosensor for nitrite was developed by immobilization of hemoglobin (Hb) and a room temperature ionic liquid, 1-ethyl-3-methyl imidazolium tetrafluoroborate (EMIT), on a multi-walled carbon nanotubes (MWNTs) modified electrode. Compared with Hb/MWNTs modified electrode, the Hb/RTILs/MWNTs modified electrode showed better electrochemical response, indicating that EMIT can promote direct electron transfer of Hb. A pair of well-defined, quasi-reversible redox peaks of Hb with a formal potential of −0.315 V was observed. The immobilized Hb exhibited remarkable electrocatalytic activity for the reduction of nitrite. The linear response range was from 4.0 × 10−6 to 3.2 × 10−4 M with a detection limit of 8.1 × 10−7 M at a signal-to-noise ratio of 3. The resulting biosensor has been successfully applied to the determination of nitrite in water samples.

Keywords

Hemoglobin RTILs EMIT MWNTs Biosensor 
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Notes

Acknowledgements

The authors thank Professor T. Ohsaka of Tokyo Institute of Technology for providing RTIL, EMIT. This work was supported by the National Natural Science Foundation and Program for Innovative Research Team in Anhui Normal University.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.School of Environmental ScienceAnhui Normal UniversityWuhuPeople’s Republic of China
  2. 2.Anhui Key Laboratory of Chem-Biosensing, School of Chemistry and Materials ScienceAnhui Normal UniversityWuhuPeople’s Republic of China

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