Microchimica Acta

, Volume 162, Issue 1–2, pp 57–64 | Cite as

Preparation of porous chitosan/carbon nanotubes film modified electrode for biosensor application

Original Paper


A simple and controllable electrodeposition method is described to fabricate a homogeneous porous chitosan/single-walled carbon nanotubes (CHIT/SWNTs) nanocomposite film. The thickness of the nanocomposite film can be controlled through the change of concentration of SWNTs, SiO2 nanoparticles and chitosan solution, deposition time. Glucose oxidase (GOx) served as a model enzyme to demonstrate the potential application of the macroporous structured films in fabrication of amperometric glucose sensor with negligible mass transport limitation. The glucose biosensor was constructed by entrapping GOx molecules to the porous SWCNTs/CHIT nanocomposite film using glutaraldehyde as a cross-linker. The fabricated biosensor with three-dimension porous structures can provide a biocompatible microenvironment for maintaining the bioactivity of the immobilized enzyme, enhance mass transport of glucose substrate, and increase enzyme loading. Therefore, the biosensor exhibits a rapid response (<5 sec), a wide linear range (10 µM to 35 mM) and a low detection limit of 2.5 µM.

Keywords: Nanocomposite film; chitosan; porous structure; carbon nanotubes; SiO2 nanoparticles; biosensor; glucose 


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Department of ChemistryNanchang UniversityNanchangP.R. China

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