Microchimica Acta

, Volume 160, Issue 4, pp 421–426 | Cite as

Biochemical sniffer with choline oxidase for measurement of choline vapour

  • Hiroyuki Kudo
  • Teruyoshi Goto
  • Takao Saito
  • Hirokazu Saito
  • Kimio Otsuka
  • Kohji Mitsubayashi
Original Paper
First Online:
Received:
Accepted:

Abstract.

An enzyme-based gas sensor (bio-sniffer) for choline vapour was fabricated and tested. The bio-sniffer was constructed using a Clark-type dissolved oxygen electrode and an enzyme (choline oxidase) immobilized membrane. This bioelectronic device measures choline concentration by the oxygen consumption induced by an enzyme reaction of choline oxidase. As the assessment of sensor performances, the calibration curves for choline in the liquid and gas phases were investigated, respectively. The responses of the bioelectronic device to choline solutions of various concentrations were related within a range from 5.00 to 700 µmol·L−1 with a correlation coefficient of 0.999. On the other hand, the bio-sniffer for choline vapour was placed into a gas-measuring chamber and calibrated using gas detection tubes. The calibration range was 1.00–30.0 ppm (correlation coefficient: 0.996). The response time for choline vapour was approximately 15% slower than that of biosensor for choline solution. These results indicate that the bio-sniffer is useful to monitor colourless and odourless choline gas released from coating compositions including choline.

Key words: Biosensor; enzyme immobilization; choline; amperometoric; gas sensor 
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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Hiroyuki Kudo
    • 1
  • Teruyoshi Goto
    • 2
  • Takao Saito
    • 1
  • Hirokazu Saito
    • 1
  • Kimio Otsuka
    • 1
  • Kohji Mitsubayashi
    • 1
  1. 1.Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and BioengineeringTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Graduate School of EngineeringTokai UniversityHiratsukaJapan

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