Microchimica Acta

, Volume 153, Issue 1–2, pp 15–20 | Cite as

PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study

Article

Abstract.

A 4-pyridyldithiol-derivatized peptide nucleic acid (PNA), which was designed to recognize a specific gene, was attached to 3-mercapropropyloxysilane coated magnetic nanoparticles (MNPs) via a thiol-disulfide exchange reaction. Subsequently, PNA-functionalized magnetic nanoparticles (PMNPs) were prepared. Sequentially the PMNPs were challenged with non-complementary and perfect-match DNA targets. The entire procedure was monitored using surface-enhanced Raman scattering (SERS). The results showed that the PMNPs were able to efficiently hybridize with the perfect-match ssDNA target and showed no affinity towards non-complementary DNA. This approach may provide a means of direct and label-free gene analysis.

Key words: Peptide nucleic acid (PNA); magnetic nanoparticles (MNPs); PNA-modified MNPs (PMNPs); surface-enhanced Raman scattering (SERS). 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Seeman, N C 2001Nano Lett122CrossRefGoogle Scholar
  2. Dai, J P H 2003Proc Natl Acad Sci U.S.A1004984CrossRefGoogle Scholar
  3. Hahm, J, Lieber, C 2004Nano Lett41CrossRefGoogle Scholar
  4. Katz, E, Willner, I 2004Angew Chem Int Ed436042Google Scholar
  5. Wang, J, Kawde, A N, Erdem, A, Salazar, M 2001Analyst1262020Google Scholar
  6. Wang, J, Xu, D, Erdem, A, Polsky, R, Salazar, M A 2002Talanta56931Google Scholar
  7. Palecek, E, Fojta, M, Jelen, F 2002Bioelectrochemistry5685CrossRefGoogle Scholar
  8. Chakrabarti, R, Klibanov, A M 2003J Am Chem Soc12512531CrossRefGoogle Scholar
  9. Egholm, M, Buchardt, O, Christensen, L, Behrens, C, Freier, S M, Driver, D A, Berg, R H, Kim, S K, Norden, B, Nielsen, P E 1993Nature365566CrossRefGoogle Scholar
  10. Ratilainen, T, Holme’n, A, Tuite, E, Nielsen, P E, Norde’n, B 2000Biochemistry397781CrossRefGoogle Scholar
  11. Igloi, G L 1998Proc Natl Acad Sci958562CrossRefGoogle Scholar
  12. Shen, H B, Xia, J F, Yang, H F 2001Spectroscopy Spectral Anal (in Chinese)21798Google Scholar
  13. Isola, N, Stokes, D L, Vo-Dinh, T 1998Anal Chem701352CrossRefGoogle Scholar
  14. Vo-Dinh, T, Allain, L R, Stokes, D L 2002J Raman Spectrosc33511CrossRefGoogle Scholar
  15. Allain, L R, Vo-Dinh, T 2002Anal Chim Acta469149CrossRefGoogle Scholar
  16. Culha, M, Stokes, D L, Allain, L R, Vo-Dinh, T 2003Anal Chem756196CrossRefGoogle Scholar
  17. Graham, D, Smith, W E, Linacre, A M T, Munro, C H, Watson, N D, White, P C 1997Anal Chem694703CrossRefGoogle Scholar
  18. Santra, S, Tapec, R, Theodoropoulou, N, Bobson, J, Hebard, A, Tan, W 2001Langmuir172900CrossRefGoogle Scholar
  19. Hernandez, G, Rodriguez, R 1999J Non-Cryst Solids246209CrossRefGoogle Scholar
  20. Colthup, N B, Daly, L H, Wiberley, S E 1990Introduction to infrared and raman spectroscopy, 3rd edn.Academic PressNew York136Google Scholar
  21. Galeener, F L, Mickelsen, J C, Johnson, N M 1978The physics of SiO2 and its interfaces.PergamonNew York221Google Scholar
  22. Yoshikawa, M, Iwagami, K, Morita, N, Matsunobe, T, Ishida, H 1997Thin Solid Films310167CrossRefGoogle Scholar
  23. Pan, J L 1986The applications of raman spectrum in organic chemistry (in Chinese).Chemistry Industry PressBeijing75Google Scholar
  24. Suh, J S, Moskovits, M 1986J Am Chem Soc1084711Google Scholar
  25. Shen, H B, Zhou, W J, Yang, H F 2002Acta Phys Chin Sin (in Chinese)18490Google Scholar
  26. Herne, T M, Ahern, A M, Carrell, R L 1991J Am Chem Soc113846CrossRefGoogle Scholar
  27. Dou, X M, Jung, Y M, Yamamoto, H, Doi, S, Ozaki, Y 1999Appl Spectroscopy53133Google Scholar
  28. Guo, L, Huang, Q J, Li, X Y, Yang, S 2001Phys Chem Chem Phys31661CrossRefGoogle Scholar
  29. Zhao, Y D, Pang, D W, Hu, S, Wang, Z L, Cheng, J K, Qi, Y P, Dai, H P, Mao, B W, Tian, Z Q, Luo, J, Lin, Z H 1999Anal Chim Acta38893Google Scholar
  30. Dollish, F R, Fateley, W G, Bentley, F F 1974Characteric raman frequencies of organic compounds.WileyNew York92Google Scholar
  31. Dou, X M, Jung, Y M, Cao, Z Q, Ozaki, Y 1999Appl Spectroscopy531440Google Scholar
  32. Thomas, G J,Jr, Wang, A H J 1988Nucleic Acids & Molecular Biol21Google Scholar
  33. Tsuboi, M, Takahashi, S, Muraishi, S, Kajiura, T 1971Bull Chem Japan442921Google Scholar
  34. Small, E W, Peticolas, W L 1971Biopolymers1069Google Scholar
  35. Ratilanien, T, Holmen, A, Tuite, E, Haaima, G, Christensen, L, Nielsen, P E, Norden, B 1998Biochemistry3712331Google Scholar
  36. Saviano, M, Romanelli, A, Bucci, E, Pedone, C, Mischiati, C, Bianchi, N, Feriotto, G, Borgatti, M 2000R J Biomol Struct Dyn18353Google Scholar
  37. Ke, W H, Yu, D W, Wu, J Z 1999Spectrochim Acta, Part A551081Google Scholar

Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  1. 1.College of Life and Environment Science, Shanghai Normal UniversityShanghaiChina

Personalised recommendations