Microchimica Acta

, Volume 146, Issue 3–4, pp 297–302 | Cite as

Solid Phase Extraction and Spectrophotometric Determination of Mercury in Tobacco and Tobacco Additives with p-Sulfobenzylidenerhodanine as Chromogenic Reagent

  • Yunhuai Zhang
  • Guanyu Yang
  • Qiufen Hu
  • Yin Jiayuan
  • Zhong Li
Article

Abstract.

A highly sensitive, selective and rapid method for the determination of mercury based on the reaction of mercury(II) with p-sulfobenzylidenerhodanine (SBDR) and the solid phase extraction of the colored chelate with a C18 cartridge has been developed. In the presence of pH 3.8 acetate buffer solution and Tween-80 medium, SBDR reacts with mercury to form a red chelate of a molar ratio of 1:2 (mercury to SBDR). This chelate was enriched by solid phase extraction with a C18 cartridge and eluted from the cartridge with ethanol (containing 5% acetic acid). The enrichment factor of 50 was achieved. In the ethanol medium, the molar absorptivity of the chelate is 1.28×105 L·mol−1·cm−1 at 545 nm. Beer’s law is obeyed in the range of 0.01 ∼ 1.2 µg mL−1 in the measured solution. The relative standard deviation for eleven replicate samples of 0.01 µg mL−1 level is 1.52%. This method can be applied to the determination of mercury in tobacco and tobacco additives with good results.

Key words: Mercury; solid phase extraction spectrophotometry; p-sulfobenzylidenerhodanine. 

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Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  • Yunhuai Zhang
    • 1
  • Guanyu Yang
    • 2
  • Qiufen Hu
    • 2
  • Yin Jiayuan
    • 2
  • Zhong Li
    • 1
  1. 1.College of Chemistry & Engineering, Chongqing UniversityChongqingP.R. China
  2. 2.Key Laboratory of Chemistry & Engineering, Yunnan Academy of Tobacco ScienceKunmingP.R. China

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