Cigarette smoking as an aggravating factor in inflammatory tissue-destructive diseases. Increase in tumor necrosis Factor-α priming of peripheral neutrophils measured as generation of oxygen radicals

  • A. Gustafsson
  • B. Åsman
  • K. Bergström
Original

Abstract

Stimulated neutrophils from subjects with various inflammatory tissue-destructive conditions, such as periodontal, pulmonary, gastrointestinal, and cardiovascular diseases, generate more oxygen radicals and proteases, implicated in tissue destruction, than neutrophils from healthy persons. Cigarette smoking aggravates these diseases. The aim of our study was to investigate the effect of cigarette smoking on the priming capacity of tumor necrosis factor-α, measured as generation of radicals from stimulated neutrophils, in smoking and non-smoking subjects with or without periodontitis. The priming effect was higher in neutrophils from smokers. In the group with periodontitis, smoking caused an even greater increase in the generation of radicals, indicating an additive effect of this local disease. The membrane expression of CD11b, CD15, and CD63 was significantly higher on neutrophils from smokers, indicating upregulated neutrophil functions. This increased priming effect of tumor necrosis factor-α in smokers subjects could be of importance in the aggravation of tissue-destructive inflammatory diseases.

Key words

Tumor necrosis factor-α Neutrophils Tissue destruction Cigarette smoking 

References

  1. 1.
    Bartecchi CE, MacKenzie TD, Schrier RW. The human costs of tobacco use (1). N Engl J Med 1994; 330:907.PubMedCrossRefGoogle Scholar
  2. 2.
    Tonetti MS. Cigarette smoking and periodontal diseases: etiology and management of disease. Ann Periodontol 1998; 3:88.PubMedGoogle Scholar
  3. 3.
    Papapnaou PN. Periodontal diseases: epidemiology. Ann Periodontol 1996;1:1.Google Scholar
  4. 4.
    Weiss SJ. Tissue destruction by neutrophils. N Engl J Med 1989; 320:365.PubMedGoogle Scholar
  5. 5.
    Gustafsson A, Åsman B. Increased release of free oxygen radicals from peripheral neutrophils in adult periodontitis after Fcγ-receptor stimulation. J Clin Periodontol 1996; 23:38.PubMedCrossRefGoogle Scholar
  6. 6.
    Whyte GJ, Seymore GJ, Cheung K, Robison MF. Chemiluminescence of peripheral polymorphonuclear leukocytes from adult periodontitis patients. J Clin Periodontol 1989; 16:69.PubMedCrossRefGoogle Scholar
  7. 7.
    Postma DS, Renkema TE, Noordhoek JA, Faber H, Sluiter HJ, Kauffman H. Association between nonspecific bronchial hyperreactivity and superoxide anion production by polymorphonuclear leukocytes in chronic air-flow obstruction. Am Rev Respir Dis 1988; 137:57.PubMedGoogle Scholar
  8. 8.
    Venge P, Rak S, Steinholtz L, Håkansson L, Lindblad G. Neutrophil function in chronic bronchitis. Eur Respir J 1991; 4:536.PubMedGoogle Scholar
  9. 9.
    Faden H, Rossi TM. Chemiluminescent response of neutrophils from patients with inflammatory bowel disease. Dig Dis Sci 1985; 30:139.PubMedCrossRefGoogle Scholar
  10. 10.
    Griga T, Tromm A, Schwegler U, May B. Enhanced superoxide anion release of normal neutrophil granulocytes primed with sera of patients with inactive inflammatory bowel disease. Z Gastroenterol 1995; 33:345.PubMedGoogle Scholar
  11. 11.
    Mohacsi A, Kozlovszky B, Kiss I, Seres I, Fulop T Jr. Neutrophils obtained from obliterative atherosclerotic patients exhibit enhanced resting respiratory burst and increased degranulation in response to various stimuli. Biochim Biophys Acta 1996; 1316:210.PubMedGoogle Scholar
  12. 12.
    Elneihoum AM, Falke P, Hedblad B, Lindgarde F, Ohlsson K. Leukocyte activation in atherosclerosis: correlation with risk factors. Atherosclerosis 1997; 131:79.PubMedCrossRefGoogle Scholar
  13. 13.
    Steinbeck MJ, Roth JA. Neutrophil activation by recombinant cytokines. Rev Infect Dis 1989; 11:549.PubMedGoogle Scholar
  14. 14.
    Hayes C, Sparrow D, Cohen M, Vokonas PS, Garcia RI. The association between alveolar bone loss and pulmonary function: the VA Dental Longitudinal Study. Ann Periodontol 1998; 3:257.PubMedGoogle Scholar
  15. 15.
    DeStefano F, Anda RF, Kahn HS, Williamson DF, Russell CM. Dental disease and risk of coronary heart disease and mortality. BMJ 1993; 306:688.PubMedGoogle Scholar
  16. 16.
    Joshipura KJ, Rimm EB, Douglass CW, Trichopoulos D, Ascherio A, Willett WC. Poor oral health and coronary heart disease. J Dent Res 1996; 75:1631.PubMedCrossRefGoogle Scholar
  17. 17.
    Fredriksson M, Gustafsson A, Åsman B, Bergström K. Periodontitis increases chemiluminescence of peripheral neutrophils independently of priming by the preparation method. Oral Dis 1999; 5:229.PubMedGoogle Scholar
  18. 18.
    Bergström K, Åsman B. Luminol enhanced Fc-receptor dependent chemiluminescence from peripheral PMN cells. A methodological study. Scand J Clin Lab Invest 1993; 53:171.PubMedCrossRefGoogle Scholar
  19. 19.
    Figueredo CM, Gustafsson A. Activity and inhibition of elastase in GCF. J Clin Periodontol 1998; 25:531.PubMedCrossRefGoogle Scholar
  20. 20.
    Ingman T, Tervahartiala T, Ding Y, et al. Matrix metalloproteinases and their inhibitors in gingival crevicular fluid and saliva of periodontitis patients. J Clin Periodontol 1996; 23:1127.PubMedCrossRefGoogle Scholar
  21. 21.
    Fredriksson M, Figueredo CMS, Gustafsson A, Bergström KG, Åsman BE. Effect of periodontitis and smoking on blood leukocytes and acute-phase proteins. J Periodontol 1999; 70:1355.PubMedCrossRefGoogle Scholar
  22. 22.
    Kalra J, Chaudhary AK, Prasad K. Increased production of oxygen free radicals in cigarette smokers. Int J Exp Pathol 1991; 72:1.PubMedGoogle Scholar
  23. 23.
    Anderson R, Theron AJ, Richards GA, Myer MS, Van Rensburg AJ. Passive smoking by humans sensitizes circulating neutrophils. Am Rev Res Dis 1991; 144:570.Google Scholar
  24. 24.
    Berger M, Wetzler EM, Wallis RS. Tumor necrosis factor is the major monocyte product that increases complement receptor expression on mature human neutrophils. Blood 1988; 71:151.PubMedGoogle Scholar
  25. 25.
    Åsman B, Gustafsson A, Bergström K. Priming of neutrophils with tumor necrosis factor-alpha measured as Fc gamma receptor-mediated respiratory burst correlates with increased complement receptor 3 membrane density. Int J Clin Lab Res 1996; 26:236.PubMedCrossRefGoogle Scholar
  26. 26.
    Kuijpers TW, Tool AT, Schoot CE van der, Ginsel LA, Onderwater JJ, Roos D, Verhoeven AJ. Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood 1991; 78:1105.PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • A. Gustafsson
    • 1
    • 2
  • B. Åsman
    • 1
  • K. Bergström
    • 1
  1. 1.Karolinska Institutet Department of Medical Laboratory Sciences and TechnologyDivision of Clinical ChemistryStockholmSweden
  2. 2.Karolinska Institutet, Department of OdontologyDivision of PeriodontologyStockholmSweden
  3. 3.Division of Periodontology, School of DentistryKarolinska InstituteHuddingeSweden

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