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The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway

  • Yuma Wada
  • Atsushi Takata
  • Tetsuya IkemotoEmail author
  • Yuji Morine
  • Satoru Imura
  • Shuichi Iwahashi
  • Yu Saito
  • Mitsuo Shimada
Original Article
  • 10 Downloads

Abstract

Purpose

Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation.

Methods

The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 µM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 µM and control group were transplanted under the kidney capsule of streptozotocin-induced diabetic ICR mice.

Results

The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p < 0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p < 0.05), the ROS production was lower (p < 0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p < 0.01).

Conclusion

EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.

Keywords

Epigallocatechin 3-gallate (EGCG) Nuclear factor erythroid 2-related factor 2 (Nrf2) Islet transplantation Reactive oxygen species (ROS) production Pre-conditioning 

Notes

Compliance with ethical standards

Conflict of interest

All authors have no conflicts of interest.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  1. 1.Department of Surgery, Graduate School of Biomedical SciencesTokushima UniversityTokushimaJapan

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