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Long-term outcomes of patch tracheoplasty using collagenous tissue membranes (biosheets) produced by in-body tissue architecture in a beagle model

  • Satoshi Umeda
  • Yasuhide Nakayama
  • Takeshi Terazawa
  • Ryosuke Iwai
  • Shohei Hiwatashi
  • Kengo Nakahata
  • Yuichi Takama
  • Hiroomi OkuyamaEmail author
Original Article
  • 36 Downloads

Abstract

Purpose

Although various artificial tracheas have been developed, none have proven satisfactory for clinical use. In-body tissue architecture (IBTA) has enabled us to produce collagenous tissues with a wide range of shapes and sizes to meet the needs of individual recipients. In the present study, we investigated the long-term outcomes of patch tracheoplasty using an IBTA-induced collagenous tissue membrane (“biosheet”) in a beagle model.

Methods

Nine adult female beagles were used. Biosheets were prepared by embedding cylindrical molds assembled with a silicone rod and a slitting pipe into dorsal subcutaneous pouches for 2 months. The sheets were then implanted by patch tracheoplasty. An endoscopic evaluation was performed after 1, 3, or 12 months. The implanted biosheets were harvested for a histological evaluation at the same time points.

Results

All animals survived the study. At 1 month after tracheoplasty, the anastomotic parts and internal surface of the biosheets were smooth with ciliated columnar epithelium, which regenerated into the internal surface of the biosheet. The chronological spread of chondrocytes into the biosheet was observed at 3 and 12 months.

Conclusions

Biosheets showed excellent performance as a scaffold for trachea regeneration with complete luminal epithelium and partial chondrocytes in a 1-year beagle implantation model of patch tracheoplasty.

Keywords

Regenerative medicine Trachea Scaffold Patch tracheoplasty Dog (beagle) 

Notes

Compliance with ethical standards

Conflict of interest

All authors declare no conflict of interest.

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Satoshi Umeda
    • 1
  • Yasuhide Nakayama
    • 2
  • Takeshi Terazawa
    • 3
  • Ryosuke Iwai
    • 4
  • Shohei Hiwatashi
    • 1
  • Kengo Nakahata
    • 1
  • Yuichi Takama
    • 1
  • Hiroomi Okuyama
    • 1
    Email author
  1. 1.Department of Pediatric SurgeryOsaka University Graduate School of MedicineOsakaJapan
  2. 2.Biotube Co., Ltd.TokyoJapan
  3. 3.Division of Cell Engineering, Graduate School of Chemical Science and EngineeringHokkaido UniversitySapporoJapan
  4. 4.Research Institute of Technology, Okayama University of ScienceOkayamaJapan

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