Nectin-1 expression in cancer-associated fibroblasts is a predictor of poor prognosis for pancreatic ductal adenocarcinoma
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Nectin-1 is a cell adhesion molecule that regulates the formation of adherens junctions and tight junctions. We measured the expression of nectin-1 in cancer-associated fibroblasts (CAFs) in patients with pancreatic ductal adenocarcinoma (PDAC).
Nectin-1 expression was measured via immunohistochemistry using tissue microarray blocks constructed from resected PDAC tissue from 258 patients. We screened for associations between nectin-1 expression and clinicopathological parameters. According to the percentage of CAFs stained, expression was classified as negative at ≤ 30% and positive at > 30%.
Nectin-1 expression was confirmed in CAFs from 64 patients (24.8%), and was associated with lymph node metastasis (p = 0.016), advanced Union for International Cancer Control stage (p = 0.016), perineural invasion (p = 0.022), pancreatic head tumors (p = 0.023), and shorter overall survival (p = 0.003). Multivariate analysis revealed that nectin-1 expression in CAFs was an independent prognostic factor (p = 0.038).
Diffuse nectin-1 expression in the CAFs of PDAC patients is associated with invasion, metastasis, and shorter survival.
KeywordsPancreatic ductal adenocarcinoma Nectin-1 Cell adhesion molecules Cancer-associated fibroblast Epithelial mesenchymal transition
We thank the Division of Diagnostic Pathology, Tokai University Hospital, and the Support Center for Medical Research and Education, Tokai University for their valuable technical assistance.
This study was supported by the Research and Study Project of the Tokai University Educational System’s General Research Organization, the Pancreas Research Foundation of Japan, and a Grant-in-Aid for Scientific Research (C) (16K08659).
Compliance with ethical standards
The study protocol was approved by the Research Ethics Committee (no. 13R244) of the Tokai University School of Medicine (Kanagawa, Japan) and has therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.
Conflict of interest
We declare that we have no conflicts of interest.
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