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Surgery Today

, Volume 41, Issue 8, pp 1075–1084 | Cite as

Matrine attenuates endotoxin-induced acute liver injury after hepatic ischemia/reperfusion in rats

  • Feng Zhang
  • Xun Wang
  • Liquan Tong
  • Haiquan Qiao
  • Xinlei Li
  • Liguang You
  • Hongchi Jiang
  • Xueying Sun
Original Article

Abstract

Purpose

Hepatic ischemia/reperfusion (HIR) injury is an unavoidable consequence of major liver surgery, during which endotoxemia often takes place. This study aimed to investigate whether matrine has a protective effect against HIR-induced liver injury aggravated by endotoxin.

Methods

Wistar rats were subjected to 30 min of HIR followed by lipopolysaccharide (LPS) (0.5 mg/kg) administration. At the indicated time points, six rats from each group (24 rats) were randomly euthanized to collect blood samples and livers.

Results

Preadministration of matrine protected livers from injury induced by HIR+LPS as the histological score, myeloperoxidase activity and malondialdehyde contents, expression of macrophage-inflammatory protein-2, DNA-binding activity of nuclear factor κB, and serum levels of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase, tumor necrosis factor-α, soluble intercellular adhesion molecule-1, and nitric oxide were significantly reduced, and serum levels of interleukin-6 were further increased. HIR+LPS markedly induced cell apoptosis and necrosis, and upregulated the expression of cleaved caspase-3, Fas, and FasL. Matrine significantly reduced cell necrosis, but had a nonsignificant inhibitory effect on cell apoptosis and expression of cleaved caspase-3 and FasL

Conclusions

Matrine attenuates endotoxin-induced acute liver injury after HIR mainly by its anti-inflammatory and antioxidative activities, and has little inhibitory effect on cell apoptosis.

Key words

Matrine Endotoxemia Hepatic ischemia/reperfusion Inflammatory cytokine Oxidative stress Apoptosis 

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Copyright information

© Springer 2011

Authors and Affiliations

  • Feng Zhang
    • 1
  • Xun Wang
    • 2
  • Liquan Tong
    • 1
  • Haiquan Qiao
    • 3
  • Xinlei Li
    • 1
  • Liguang You
    • 1
  • Hongchi Jiang
    • 3
  • Xueying Sun
    • 3
  1. 1.Department of General Surgerythe Fifth Affiliated Hospital of Harbin Medical UniversityDaqingChina
  2. 2.Department of General SurgeryDaqing Oilfield General HospitalDaqingChina
  3. 3.Department of General Surgery, Hepatosplenic Surgery Centerthe First Affiliated Hospital of Harbin Medical UniversityHarbinChina

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