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Surgery Today

, Volume 39, Issue 6, pp 514–517 | Cite as

Effect of electrolyzed strong acid water on peritoneal irrigation of experimental perforated peritonitis

  • Akio Kubota
  • Keisuke Nose
  • Takeo Yonekura
  • Takuya Kosumi
  • Katsuji Yamauchi
  • Harumasa Oyanagi
Original Article

Abstract

Purpose

Electrolyzed strong acid water (ESAW) is generated by the electrolysis of a weak sodium chloride solution. Although ESAW is known to have a strong bactericidal activity and to be harmless to the living body, its effectiveness and safety in the treatment of perforated peritonitis has not been well established.

Methods

Male Wistar rats were used for the study. Three hours after cecal ligation and puncture, the cecum was resected and the peritoneal cavity was irrigated with 50 ml of saline (Group S, n=12) or ESAW (Group E, n=14). The 5-day survival rate was compared between the two groups. In another pair of animals (n=10 each), bacteria in the ascitic fluid were counted at 6 and 18 h after irrigation.

Results

No adverse effects of ESAW were observed in the experimental group. The 5-day survival rate was 25% (3/12) and 85.7% (12/14) in Groups S and E, respectively (P < 0.01). The bacterial count at 18 h after the irrigation in Groups S and E was (5.0 ± 2.5) × 105/ml and (2.2 ± 2.0) × 104/ml, respectively (P < 0.0001).

Conclusion

Peritoneal lavage with ESAW had no adverse effect, and achieved more effective decontamination than saline for perforated peritonitis. Therefore, the results of this study are considered to warrant and support the clinical application of ESAW.

Key words

Perforated peritonitis Peritoneal lavage Electrolyzed strong acid water Hypochlorous acid water Bactericidal activity 

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References

  1. 1.
    Kumon K. What is functional water? Artif Organs 1997;21:2–4.PubMedGoogle Scholar
  2. 2.
    Nakagawara S, Goto T, Nara M, Ozawa Y, Hotta K, Arata Y. Spectroscopic characterization and the pH dependence of bactericidal activity of the aqueous chlorine solution. Analytical Sciences 1998;14:691–698.CrossRefGoogle Scholar
  3. 3.
    Hotta K, Kawaguchi K, Saitoh F, Saito N, Suzuki K, Ochi K, et al. Antimicrobial activity of electrolyzed NaCl solutions: effect on the growth of Streptomyces spp. Actinomycetol 1994;l8:51–56.CrossRefGoogle Scholar
  4. 4.
    Iwasawa A, Nakamura Y. Bactericidal effect of acidic electrolyzed water-comparison of chemical acidic sodium hydrochloride (NaOCl) solution (in Japanese with English abstract). Kansenshogaku Zashi (J Jpn Assoc Infect Dis) 1996;70:915–922.Google Scholar
  5. 5.
    Kiura H, Sano K, Morimatsu S, Nakano T, Morita C, Yamaguchi M, et al. Bactericidal activity of electrolyzed acid water from solution containing sodium chloride at low concentration, in comparison with that at high concentration. J Microbiol Methods 2002;49:285–293.PubMedCrossRefGoogle Scholar
  6. 6.
    Abe S, Miya Y, Okuda R. Inactivation of hepatitis B virus by high oxidation potential water (in Japanese with English abstract). Nihon Shikahozongaku Zasshi (Jpn J Conserv Dent) 1994;37:1616–1623.Google Scholar
  7. 7.
    Morita C, Sano K, Morimatsu S, Kiura H, Goto T, Kohno T, et al. Disinfection potential of electrolyzed solutions containing sodium chloride at low concentrations. J Virol Methods 2000;85:163–174.PubMedCrossRefGoogle Scholar
  8. 8.
    Tagawa M, Yamaguchi T, Yokosuka O, Matsutani S, Maeda T, Saisho H. Inactivation of a hepadnavirus by electrolyzed acid water. J Antimicrob Chemother 2000;46:363–368.PubMedCrossRefGoogle Scholar
  9. 9.
    Nakae H, Inada AH. Effectiveness of electrolyzed oxidized water irrigation in a burn-wound infection model. J Trauma 2000;49:511–514.PubMedCrossRefGoogle Scholar
  10. 10.
    Shimomura S, Matsumoto K, Yaguchi H, Okuda T, Miyajima S, Negi A, et al. Acidic electrolysed water in the disinfection of the ocular surface. Exp Eye Res 2000;70:1–6.CrossRefGoogle Scholar
  11. 11.
    Hayashi H, Kumon K, Yahagi N, Haruna M, Qatanabe Y, Matsui J, et al. Successful treatment of mediastinitis after cardiovascular surgery using electrolyzed strong acid aqueous solution. Artif Organs 1997;21:39–42.PubMedCrossRefGoogle Scholar
  12. 12.
    Kubota A, Hoki M, Yonekura T, Nose K, Hirooka S, Kosumi T, et al. Effectiveness of acid oxidative potential water in peritoneal lavage for perforated appendicitis. Asian J Surg 1999;22:282–283.Google Scholar
  13. 13.
    Takemura M, Iwamoto K, Goshi S. intraoperative irrigation with super oxidation water for perforated appendicitis (in Japanese). Geka (Jpn J Surg) 1999;61:1303–1305.Google Scholar
  14. 14.
    Ohuchi S, Kawazoe K, Ishihara K, Izumoto H, Eishi K. Management with closed irrigation for post-sternotomy mediastinitis. Jpn J Thorac Cardiovasc Surg 2003;511–514.Google Scholar
  15. 15.
    Wichterman KA, Baue AE, Chaudry IH. Sepsis and septic shock; a review of laboratory models and proposal. J Surg Res 1980;29:189–201.PubMedCrossRefGoogle Scholar
  16. 16.
    Hotta K. Acid electrolyzed saline solution: its function and medical application. J Jpn Soc Intensive Care Med 2000;7:97–105.Google Scholar
  17. 17.
    Iwasawa A, Nakamura Y, Mizuno T. Bactericidal activity of aqua oxidation water against clinically isolated strains (in Japanese). Nihon Kankyo-Kansensyogaku Zasshi (J Jpn Environ Infect) 1993;8:11–16.Google Scholar
  18. 18.
    Tanaka N, Fujisawa T, Daimon T, Fujiwara, Yamamoto M, Abe T. The cleaning and disinfecting of hemodialysis equipment using electrolyzed strong acid aqueous solution. Artif Organs 1999;23:303–309.PubMedCrossRefGoogle Scholar
  19. 19.
    Iwasawa A, Nakamura Y. Cytotoxic effect of antiseptics: comparison In vitro, in vivo examination of strong acidic electrolyzed water, povidone-iodine, chlorhexidine and benzalkonium chloride (in Japanese with English abstract). Kansensyogaku Zasshi (J Jpn Assoc Infect Dis) 2003;77:316–322.Google Scholar
  20. 20.
    Sakurai Y, Ogoshi K, Okubo T, Kaku M, Kobayashi I. Strong acidic electrolyzed water: valuable disinfectant of endoscopes. Dig Endoscopy 2002;14:61–66.CrossRefGoogle Scholar
  21. 21.
    Otoguro K, Suzuki F, Akimaru Y, Iijima H, Yajima Y, Uebaba K, et al. Hand disinfectant activities of two kinds of electrolysed acid aqueous solutions by globe juice method (in Japanese with English abstract). Nihon Kankyo Kansensyogaku Zasshi (J Jpn Environ Infect) 1996;11:117–122.Google Scholar
  22. 22.
    Sekiya S, Ohmori K, Harii K. Treatment of infectious skin defects or ulcers with electrolyzed strong acid aqueous solution. Artif Organs 1997;21:32–38.PubMedGoogle Scholar
  23. 23.
    Gurleyik G, Yanikkaya G, Gurleyik E, Ozturk E, Dulundu E, Saglam A. Effects of granulocyte-colony stimulation factor on the polymorphonuclear leukocyte activity and the course of sepsis in rats with experimental peritonitis. Surg Today 2007;37:401–405.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2009

Authors and Affiliations

  • Akio Kubota
    • 1
  • Keisuke Nose
    • 2
  • Takeo Yonekura
    • 2
  • Takuya Kosumi
    • 2
  • Katsuji Yamauchi
    • 2
  • Harumasa Oyanagi
    • 2
  1. 1.Department of Pediatric SurgeryOsaka Medical Center and Research Institute for Maternal and Child HealthIzumi, OsakaJapan
  2. 2.Division of Pediatric SurgeryKinki University School of MedicineOsakasayama, OsakaJapan

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