Surgery Today

, Volume 37, Issue 5, pp 406–411 | Cite as

Effect of a Low Molecular Weight Heparin Molecule, Dalteparin, on Cellular Apoptosis and Inflammatory Process in an Incisional Wound-Healing Model

  • Ali Civelek
  • Koray Ak
  • Ozlem Kurtkaya
  • Atike Tekeli
  • Selim Isbir
  • Erol Nargileci
  • Sinan Arsan
  • Aydin Sav
Original Articles

Abstract

Purpose

In this study we aimed to test the effect of a low molecular weight heparin molecule, namely dalteparin, on the inflammation and cellular apoptosis in an incisional wound-healing model in rats.

Methods

Eighteen male Sprague–Dawley rats were randomly assigned to three groups (n = 6 for each group). Two full-thickness skin incisions were made over cervical and lumbar regions of all rats. Group 1 (sham group) received no treatment, group 2 (control group) received 0.01 ml/g saline subcutaneously 12 h two times daily from 0 to 10th postoperative day, and group 3 (dalteparin group): received 1 IU/g dalteparin subcutaneously two times daily from 0 to 10th postoperative day. A histological evaluation was done by light microscopy. Apoptosis was detected immunohistochemically by anti-poly (ADP-ribose) polymerase p85 fragment pAb.

Results

The early inflammatory response and related tissue edema were depressed on day 3 in the dalteparin group when compared with those in the other groups (P < 0.05). Fibroblast proliferation was also depressed on day 10 in the dalteparin group compared to the others (P < 0.05). Furthermore, increased apoptosis was detected in the dalteparin group both on day 3 and day 10.

Conclusion

Our results showed that dalteparin may adversely affect the incisional wound healing by suppressing the early inflammatory process and increasing cellular apoptosis; however, further studies are warranted to confirm the results.

Key words

Low molecular weight heparin Wound healing Inflammatory process Apoptosis 

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Copyright information

© Springer-Verlag Tokyo 2007

Authors and Affiliations

  • Ali Civelek
    • 1
  • Koray Ak
    • 1
  • Ozlem Kurtkaya
    • 2
  • Atike Tekeli
    • 1
  • Selim Isbir
    • 1
  • Erol Nargileci
    • 1
  • Sinan Arsan
    • 1
  • Aydin Sav
    • 2
  1. 1.Department of Cardiovascular SurgeryMarmara University School of Medicine, Kalp Damar Cerrahisi ADIstanbulTurkey
  2. 2.Department of PathologyMarmara University School of MedicineIstanbulTurkey

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