Effect of Peritoneal Lavage with Taurolidine on Primary Colonic Anastomosis in a Rat Model of Secondary Peritonitis
The reported antibacterial, antiendotoxic, and antiadhesive effects of taurolidine prompted us to study the benefits of intraperitoneal lavage with taurolidine on primary colonic anastomosis in a rat model of secondary peritonitis.
We induced peritonitis in 40 rats by injecting Escherichia coli isolate intraperitoneally. We performed colonic resection and primary anastomosis 5 h later, after lavage with either taurolidine or saline solution. After the rats were killed, on postoperative day (POD) 3 (n = 10) or 7 (n = 10), we measured the bursting pressures and hydroxyproline levels, then examined the resected specimens histologically.
Bursting pressures and tissue hydroxyproline levels were significantly higher in the taurolidine group than in the control group on PODs 3 and 7 (P < 0.05). Histopathological examination revealed significantly higher fibroblastic activity in the taurolidine group.
The higher bursting pressures and tissue hydroxyproline levels in the rats given taurolidine showed the positive effect of taurolidine on anastomotic strength in secondary peritonitis. Taurolidine is a novel antibiotic with both antibacterial and antiendotoxic effects. Intraperitoneal lavage with taurolidine solution may reduce the risks associated with performing primary colonic anastomosis in patients with secondary peritonitis.
Key wordsTaurolidine Secondary peritonitis Colonic anastomosis
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- 1.Erb, F, Imbenotte, M, Huvenne, J, Strongerst, F 1983Structural investigations of a new organic antimicrobial: taurolidine. Analytical study and application to identification and qualification in biological fluidsEur J Drug Metab Pharmacokinet816371Google Scholar
- 2.Braumann, C, Ordemann, J, Kilian, M, Wenger, FA, Jacobi, CA 2003Local and systemic chemotherapy with taurolidine and taurolidine/heparin in colon cancer-bearing rats undergoing laparotomyClin Exp Metastas2038794Google Scholar
- 4.Seidenschnur, AM, Bech, K 2001Peroperative lavage and primary anastomosis in acute left sided colon ileusUsegkr Laeger163248992Google Scholar
- 7.Meyer, C, Rohr, S, Iderne, A, Tiberio, G, Bourtoul, C 1997The value of peroperative colonic lavage in urgent colonic surgery. Apropos of 54 patientsJ Chir (Paris)1342714Google Scholar
- 9.Turkcapar, AG, Demirer, S, Sengul, N, Ersoz, S, Kuterdem, E 2000The adverse effects of octreotide on the healing of colonic anastomoses in ratsSurg Today304215Google Scholar
- 10.Solomkin, JS, Wittman, DW, West, MA, Barie, PS 1999(2)Intraabdominal infectionsSchwartz, IS eds. Principles of surgery7th edMcGraw-HillNew York151551Google Scholar
- 11.Konukoğlu, D, İynem, H, Ziylan, E 1999Antioxidant status in experimental peritonitis: effects of alpha tocoferol and taurolinPharmacol Resw Mar3924751Google Scholar
- 13.Popovic, M, Dugalni, D, Milicevic, M 1991Use of taurolin in the prevention of septic complications in emergency abdominal surgeryActa Chir İogosl382333Google Scholar
- 15.Cronin, K, Jackson, DS, Dunphy, JE 1968Specific activity of hydroxyproline tritium in the healing colonSurg Gynecol Obstet126010615Google Scholar