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Acta Diabetologica

, Volume 38, Issue 2, pp 71–76 | Cite as

Acute hyperglycemia and acute hyperinsulinemia decrease plasma fibrinolytic activity and increase plasminogen activator inhibitor type 1 in the rat

  • A. Pandolfi
  • A. Giaccari
  • C. Cilli
  • M. M. Alberta
  • L. Morviducci
  • E. A. De Filippis
  • A. Buongiorno
  • G. Pellegrini
  • G. Capani
  • A. Consoli
Original

Abstract

Decreased plasma fibrinolysis may contribute to accelerated atherothrombosis in diabetes. To observe whether hyperglycemia and hyperinsulinemia, common findings in type 2 diabetes, acutely affect plasma fibrinolysis in vivo, we evaluated plasma fibrinolysis (lysis of fibrin plates, free PAI-1 activity and t-PA activity) in the rat after a hyperglycemic euinsulinemic clamp (n=8), an euglycemic hyperinsulinemic clamp (n=7) or a saline infusion (n=15). Plasma fibrinolytic activity was sharply reduced after both the hyperglycemic and hyperinsulinemic clamps as compared to the respective controls (mean lysis areas on the fibrin plate, 139±21 vs. 323±30 mm2, p<0.001; 78±27 vs. 312±27 mm2p<0.001, respectively). Plasma PAI-1 activity was greater after both hyperglycemic and hyperinsulinemic clamps as compared to saline infusion (6.6±2.6 vs. 1.6±0.6 IU/ml, p<0.001; 26±4 vs. 1.3±0.7 IU/ml, p<0.0001, respectively). Plasma t-PA activity was significantly reduced both after the hyperglycemic (0.36±0.15 vs. 2.17±0.18 IU/ml in controls, p<0.001) and the hyperinsulinemic (0.3±0.1 vs. 2.3±0.3 IU/ml in control, p<0.001) clamps. These data show that in vivo both acute hyperglycemia and acute hyperinsulinemia can decrease plasma fibrinolytic potential and that this is due to increased plasma PAI-1 and decreased free t-PA activities.

Key words Diabetes mellitus Hyperglycemia Hyperinsulinemia Fibrinolysis Plasminogen activator inhibitor 1 

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Copyright information

© Springer-Verlag Italia 2001

Authors and Affiliations

  • A. Pandolfi
    • 1
  • A. Giaccari
    • 2
  • C. Cilli
    • 1
  • M. M. Alberta
    • 1
  • L. Morviducci
    • 2
  • E. A. De Filippis
    • 1
  • A. Buongiorno
    • 3
  • G. Pellegrini
    • 1
  • G. Capani
    • 1
  • A. Consoli
    • 1
  1. 1.Department of Medicine and Aging Science, University of Chieti, Via dei Vestini 1, I-66100 Chieti, ItalyIT
  2. 2.Catholic University, Rome, ItalyIT
  3. 3.Istituto Superiore di Sanità, Rome, ItalyIT

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