Acta Diabetologica

, Volume 50, Issue 2, pp 201–208 | Cite as

Oxidative stress and antioxidant status in patients with late-onset gestational diabetes mellitus

  • Cristina López-Tinoco
  • Mar Roca
  • Amor García-Valero
  • Mora Murri
  • Francisco J. Tinahones
  • Carmen Segundo
  • José L. Bartha
  • Manuel Aguilar-DiosdadoEmail author
Original Article


The relationship between late-onset gestational diabetes mellitus [GDM] and oxidative stress is not well known, and the importance of the oxidant/antioxidant equilibrium in the clinical evolution and its complications require elucidation. The aim of the study was to evaluate the relationships between maternal levels of markers of oxidative stress in women with late-onset GDM that, potentially, may have considerable clinical implications in the pathogenesis and/or the evolution of GDM. Pregnant women (n = 78; 53 with GDM, 25 controls), between the 24th and 29th week of gestation, were enrolled. Both groups were analysed for demographic data, perinatal and obstetrics outcomes together with the levels of the marker’s oxidative stress and antioxidant status. Control versus patient results in the univariate analysis were the following: pre-gestational body mass index [BMI] 23.31 ± 4.2 vs. 27.13 ± 4.6 kg/m2 (P = 0.001); weeks at delivery 39.2 ± 3.05 vs. 38.9 ± 1.8 (P = 0.09); Caesarean delivery 12.5 vs. 43% (P = 0.004); macrosomia 4 vs. 9.4% (P = 0.6); lipoperoxides [LPO] 2.06 ± 1.00 vs. 3.14 ± 1.55 μmol/mg (P = 0.001); catalase 3.23 ± 1.41 vs. 2.52 ± 1.3 nmol/min/ml (P = 0.03); superoxide dismutase [SOD] 0.11 ± 0.04 vs. 0.08 ± 0.01 U/ml (P = 0.0003); glutathione peroxidase [GPX] 0.03 ± 0.006 vs. 0.025 ± 0.006 nmol/min/ml (P = 0.01); glutathione reductase [GSH] 0.004 ± 0.002 vs. 0.004 ± 0.004 nmol/min/ml (P = 0.9)]; and glutathione transferase [GST] 0.0025 ± 0.0012 vs. 0.0027 ± 0.00017 nmol/min/ml (P = 0.7). Multivariate analysis showed catalase might have a protective effect against GDM development and LPO seems to be a risk factor for the disease. These data suggest an increase in oxidative stress and a decrease in antioxidative defence in women with late-onset GDM and, as such, may have considerable clinical implications in the pathogenesis and/or the course of the pregnancy in these patients.


Gestational diabetes mellitus Markers of oxidative stress Pregnancy 



Analysis of variance


Body mass index


Diabetes mellitus type 1


Diabetes mellitus type 2


Diastolic blood pressure


Gestational diabetes mellitus


Glutathione peroxidase


Glutathione reductase


Glutathione transferase


Large-for-gestational age






Metabolic syndrome


Nuclear factor-kB


Odds ratio


Reactive oxidative substances


Standard deviation


Standard error


Superoxide dismutase


Systolic blood pressure


Thiobarbituric acid reactive substances


Total antioxidant capacity





This study was financed, in part, by grants from the Andalusia Department of Health (CTS-368).

Conflict of interest

The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work. Editorial assistance was by Dr Peter R Turner.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Cristina López-Tinoco
    • 1
  • Mar Roca
    • 3
  • Amor García-Valero
    • 1
  • Mora Murri
    • 3
  • Francisco J. Tinahones
    • 3
  • Carmen Segundo
    • 1
  • José L. Bartha
    • 2
  • Manuel Aguilar-Diosdado
    • 1
    • 4
    Email author
  1. 1.Department of EndocrinologyHospital Puerta del MarCádizSpain
  2. 2.Department of GynaecoloyHospital Puerta del MarCádizSpain
  3. 3.Department of EndocrinologyHospital Virgen de la VictoriaMálagaSpain
  4. 4.Servicio de Endocrinología y NutriciónHospital Puerta del MarCádizSpain

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