Advertisement

Acta Diabetologica

, Volume 49, Issue 3, pp 185–191 | Cite as

White rice vinegar improves pancreatic beta-cell function and fatty liver in streptozotocin-induced diabetic rats

  • Xuemei Gu
  • Hai-Lu Zhao
  • Yi Sui
  • Jing Guan
  • Juliana C. N. Chan
  • Peter C. Y. Tong
Original Article

Abstract

Vinegar is a traditional remedy for ailments including diabetes. This study was conducted to investigate the beneficial effects of vinegar in streptozotocin (STZ)-induced diabetic rats. STZ-induced diabetic rats were orally administered with white rice vinegar (WRV, 2 ml/kg body weight per day, n = 6) or with an equal volume of drinking water (n = 6) for 1 month. Fasting and random blood glucose was measured from tail vein samples. Body weight, 24-h food and water intake were monitored 1 week and 1 month after STZ injection. Fasting serum insulin concentrations were assayed using ELISA. Pancreatic beta- and alpha-cell proportions were measured using immunofluorescence microscopy. Periodic acid Schiff staining was performed to access glycogen contents and histological changes in liver tissues. Compared with control animals, the WRV-treated rats had less weight loss, lower fasting and random blood glucose, higher fasting serum insulin and higher beta-cell proportion. The WRV treatment also improved fatty changes and glycogen storages in the liver of STZ rats. Oral intake of WRV improved fasting hyperglycemia and body weight loss through attenuating insulin deficiency, pancreatic beta-cell deficit, and hepatic glycogen depletion and fatty changes in STZ-induced diabetic rats.

Keywords

Diabetes Vinegar Insulin deficiency Streptozotocin 

References

  1. 1.
    Johnston CS, Gaas CA (2006) Vinegar: medicinal uses and antiglycemic effect. Clin Nutr Obes 8:61–69Google Scholar
  2. 2.
    O’Keefe JH, Gheewala NM, O’Keefe JO (2008) Dietary strategies for improving post-prandial glucose, lipids, inflammation, and cardiovascular health. J Am Coll Cardiol 51:249–255PubMedCrossRefGoogle Scholar
  3. 3.
    Ebihara K, Nakajima A (1988) Effect of acetic acid and vinegar on blood glucose and insulin responses to orally administered sucrose and starch. Agric Biol Chem 52:1311–1312CrossRefGoogle Scholar
  4. 4.
    Ostman E, Granfeldt Y, Persson L, Bjorck I (2005) Vinegar supplementation lowers glucose and insulin responses and increases satiety after a bread meal in healthy subjects. Eur J Clin Nutr 59:983–988PubMedCrossRefGoogle Scholar
  5. 5.
    Johnston CS, Kim CM, Buller AJ (2004) Vinegar improves insulin sensitivity to a high-carbohydrate meal in subjects with insulin resistance or type 2 diabetes. Diabetes Care 27:281–282PubMedCrossRefGoogle Scholar
  6. 6.
    Johnston CS, Buller AJ (2005) Vinegar and peanut products as complementary foods to reduce postprandial glycemia. J Am Diet Assoc 105:1939–1942PubMedCrossRefGoogle Scholar
  7. 7.
    Fushimi T, Tayama K, Fukaya M et al (2001) Acetic acid feeding enhances glycogen repletion in liver and skeletal muscle of rats. J Nutr 131:1973–1977PubMedGoogle Scholar
  8. 8.
    Noel Kim, Stankovic M, Cushman T, Goldstein I, Munarriz R, Traish A (2006) Streptozotocin-induced diabetes in the rat is associated with changes in vaginal hemodynamics, morphology and biochemical markers. BMC Physiol 6:4CrossRefGoogle Scholar
  9. 9.
    Lefkowitch J (2006) Special stains in diagnostic liver pathology. Semin Diagn Pathol 23:190–198PubMedCrossRefGoogle Scholar
  10. 10.
    Schaart G, Hesselink RP, Keizer HA et al (2004) A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections. Histochem Cell Biol 122:161–169PubMedCrossRefGoogle Scholar
  11. 11.
    Johnston CS, White AM, Kent SM (2009) Preliminary evidence that regular vinegar ingestion favorably influences hemoglobin A1c values in individuals with type 2 diabetes mellitus. Diabetes Res Clin Pract 84:e15–e17PubMedCrossRefGoogle Scholar
  12. 12.
    Shishehbor F, Mansoori A, Sarkaki A, Jalali M, Latifi S (2008) Apple cider vinegar attenuates lipid profile in normal and diabetic rats. Pak J Bio Sci 11:2634–2638CrossRefGoogle Scholar
  13. 13.
    Ichikawa M, Ohta M, Kanai S et al (2003) Bitter melon malt vinegar increases daily energy turnover in rats. J Nutr Sci Vitaminol 49:428–433PubMedCrossRefGoogle Scholar
  14. 14.
    Liljeberg H, Björck I (1998) Delayed gastric emptying rate may explain improved glycaemia in healthy subjects to a starchy meal with added vinegar. Eur J Clin Nutr 52:368–371PubMedCrossRefGoogle Scholar
  15. 15.
    Ogawa N, Satsu H, Watanabe H et al (2000) Acetic acid suppresses the increase in disaccharidase activity that occurs during culture of Caco-2 cells. J Nutr 130:507–513PubMedGoogle Scholar
  16. 16.
    White AM, Johnston CS (2007) Vinegar ingestion at bedtime moderates waking glucose concentrations in adults with well-controlled type 2 diabetes. Diabetes Care 30:2814–2815PubMedCrossRefGoogle Scholar
  17. 17.
    Schnedl WJ, Ferber S, Johnson JH, Newgard CB (1994) STZ transport and cytotoxicity. Specific enhancement in GLUT2-expressing cells. Diabetes 43:1326–1333PubMedCrossRefGoogle Scholar
  18. 18.
    Brentjens R, Saltz L (2001) Islet cell tumors of the pancreas: the medical oncologist’s perspective. Surg Clin North Am 81:527–542PubMedCrossRefGoogle Scholar
  19. 19.
    Hwang SL, Liu IM, Tzeng TF, Cheng JT (2005) Activation of imidazoline receptors in adrenal gland to lower plasma glucose in streptozotocin-induced diabetic rats. Diabetologia 48:767–775PubMedCrossRefGoogle Scholar
  20. 20.
    Poitout V, Robertson RP (2008) Glucolipotoxicity: fuel excess and {beta}-cell dysfunction. Endocr Rev 29:351–366PubMedCrossRefGoogle Scholar
  21. 21.
    Barnes B, Zierath J (2005) Role of AMP–activated protein kinase in the control of glucose homeostasis. Curr Mol Med 5:341–348PubMedCrossRefGoogle Scholar
  22. 22.
    Sakakibara S, Yamauchi T, Oshima Y, Tsukamoto Y, Kadowaki T (2006) Acetic acid activates hepatic AMPK and reduces hyperglycemia in diabetic KK-A(y) mice. Biochem Biophys Res Commun 344:597–604PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Xuemei Gu
    • 1
    • 2
  • Hai-Lu Zhao
    • 2
  • Yi Sui
    • 2
  • Jing Guan
    • 2
  • Juliana C. N. Chan
    • 2
    • 3
    • 4
  • Peter C. Y. Tong
    • 3
  1. 1.Department of EndocrinologyThe First Affiliated Hospital, Wenzhou Medical CollegeWenzhouChina
  2. 2.Department of Medicine and Therapeutics, Prince of Wales HospitalThe Chinese University of Hong KongShatinHong Kong
  3. 3.Hong Kong Institute of Diabetes and ObesityThe Chinese University of Hong KongShatinChina
  4. 4.Li Ka Shing Institute of Health SciencesThe Chinese University of Hong KongShatinChina

Personalised recommendations