The impact of orthopedic device associated with carbonated hydroxyapatite on the oxidative balance: experimental study of bone healing rabbit model

  • Samira JebahiEmail author
  • Riadh Nsiri
  • Mohammed Boujbiha
  • Ezedine Bouroga
  • Tarek Rebai
  • Hassib Keskes
  • Abdelfattah El Feki
  • Hassane Oudadesse
  • Hafed El Feki
Original Article


Orthopedic devices are used in pathologic disorder as an adjunct to bone grafts to provide immediate structural stability. Unfortunately, the use of metallic devices has some complications. This study aimed to characterize the oxidative stress biomarker and the antioxidant enzyme profiles during bone regeneration. New Zealand White rabbits were divided into 4 groups: Group (I) was used as control (T), Groups II, III, and IV were used, respectively, as implanted tissue with carbonated hydroxyapatite (CHA), carbonated hydroxyapatite associated with external fixator (CHA + EF), and presenting empty defects (ED). Grafted bone tissues were carefully removed to measure malondialdehyde (MDA) concentration, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase activities (GPx). Our results showed that 4 weeks after operation, treatment of rabbits with CHA + EF showed a significantly higher malondialdehyde (MDA) concentration when compared to that of control group. The SOD, CAT, and GPx in CHA + EF group showed significantly lower activities when compared to those in control group. Eight weeks after surgery, the CHA + EF group presented a lower concentration of MDA as compared to those seen after the first 4 weeks after surgery. On the other hand, the SOD, CAT, and GPx showed a higher activity when compared with the same group. Consequently, MDA concentration and the antioxidant enzyme activities were not significant (p > 0.05) when compared to those in control group rabbits. Histologic sampling has demonstrated successful time-patterned resorption accompanied by bone replacement and remodeling. These results suggest that there was a temporary increase in the oxidative marker level in CHA + EF healing bone and the 8-week period was sufficient to re-establish oxidant–antioxidant balance accompanied by bone repair in the tibia rabbit model.


External fixator Carbonated hydroxyapatite Oxidative stress Bone regeneration 


Conflict of interest

No potential conflict of interest relevant to this article was reported.


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Copyright information

© Springer-Verlag France 2012

Authors and Affiliations

  • Samira Jebahi
    • 1
    • 2
    Email author
  • Riadh Nsiri
    • 2
  • Mohammed Boujbiha
    • 2
  • Ezedine Bouroga
    • 2
  • Tarek Rebai
    • 4
  • Hassib Keskes
    • 5
  • Abdelfattah El Feki
    • 2
  • Hassane Oudadesse
    • 1
  • Hafed El Feki
    • 3
  1. 1.University of Rennes 1, UMR CNRS 6226RennesFrance
  2. 2.Animal Ecophysiology Laboratory, Department of Life Sciences, Sfax Faculty of ScienceUniversity of SfaxSfaxTunisia
  3. 3.Science Materials and Environment Laboratory, Sfax Faculty of ScienceUniversity of SfaxSfaxTunisia
  4. 4.Histology Laboratory, Sfax Faculty of MedicineUniversity of SfaxSfaxTunisia
  5. 5.Orthopaedic and Traumatology Laboratory, Sfax Faculty of MedicineUniversity of SfaxSfaxTunisia

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