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Lung metastases regression with increased CD8+ T lymphocyte infiltration following preoperative spinal embolization and total en bloc spondylectomy using tumor-bearing frozen autograft in a patient with spinal metastatic leiomyosarcoma

  • Noritaka Yonezawa
  • Hideki Murakami
  • Apiruk Sangsin
  • Eishiro Mizukoshi
  • Hiroyuki Tsuchiya
Case Report

Abstract

Purpose

To report systemic immunological enhancement following preoperative spinal embolization and total en bloc spondylectomy (TES) using tumor-bearing frozen autograft in a patient with spinal metastatic leiomyosarcoma.

Methods

A 44-year-old woman with metastatic uterine leiomyosarcoma of the lung and L1 vertebra underwent TES following bilateral three-level preoperative segmental artery embolization. Resected tumor-bearing lamina was frozen using liquid nitrogen and used as tumor-bearing bone graft for spinal reconstruction.

Results

Tumor necrosis and obstructing material used in preoperative embolization were detected in the resected specimen of L1. Five days after TES, chest computed tomography scan demonstrated decreased solitary lung mass size without adjuvant treatment. Lobectomy was performed for the lung metastasis 42 days after TES. Infiltration of CD8+ T lymphocyte into tumor tissue significantly increased in shrunk lung metastasis. On the other hand, slight infiltration in both the resected L1 and primary uterine lesion was observed. Six months after TES, activities of daily living were normal with no evidence of local recurrence or distant metastasis. One year after TES, however, lung CT revealed occurrence of another lung metastasis, and molecular-targeting therapy (pazopanib) was initiated.

Conclusions

There were no reports demonstrating metastasis regression with CD8+ T lymphocyte infiltration after TES. This case demonstrated that preoperative tumor embolization combined with TES using tumor-bearing autograft provided both a local radical cure and systemic antitumor immunological enhancement, although the long-term effect can be limited.

Keywords

Frozen autograft Embolization CD8 Leiomyosarcoma Spondylectomy 

Notes

Acknowledgements

We are grateful to Dr. Takayuki Nojima and Dr. Hiroko Ikeda for their assistance with pathological examinations. We would also like to express my gratitude to Dr. Isao Matsumoto for resection of pulmonary metastasis. We would like to thank Editage (www.editage.jp) for English language editing. We thank Dr. Satoshi Kato and Norihiro Oku for providing clinical data and their help.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from the patient described in the study.

Supplementary material

586_2018_5831_MOESM1_ESM.pdf (3.8 mb)
Supplementary material 1 (PDF 3884 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Orthopedic SurgeryKanazawa University School of MedicineKanazawaJapan
  2. 2.Department of GastroenterologyKanazawa University HospitalKanazawaJapan

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