European Spine Journal

, Volume 25, Issue 12, pp 4008–4015 | Cite as

Intraoperative cell salvage in metastatic spine tumour surgery reduces potential for reinfusion of viable cancer cells

  • Naresh Kumar
  • Aye Sandar Zaw
  • Bee Luan Khoo
  • Sayantani Nandi
  • Zhangxing Lai
  • Gurpal Singh
  • Chwee Teck Lim
  • Jean Paul Thiery
Original Article

Abstract

Purpose

This study aimed at evaluating our hypothesis that tumour cells, which pass through the intraoperative cell salvage (IOCS) machine, lose viability due to possible injury to the cell membrane during centrifugation and filtration, enabling safe reinfusion even without filtration.

Methods

Thirteen patients who underwent metastatic spine tumour surgery (MSTS) at our institution were recruited. Blood samples (5 ml each) were collected at five different stages during surgery, namely, stage A and B: from patients’ vein during induction and at the time of maximum tumour manipulation; stage C, D and E: from the operative blood prior to IOCS processing, after IOCS processing and after IOCS-LDF (leucocyte depletion filter) processing, respectively. The samples were then analysed for viability of tumour cells using microwell-based culture.

Results

The median age of the patients was 65 years (range 37–77 years). The most common primary tumour was lung, followed by breast, hepatocellular and renal cell carcinoma. The median blood loss was 680 ml (range 300–1500 ml). Analysis of cultured blood samples showed that CTC-containing clusters were developed from some samples before IOCS-LDF processing (stage A: three patients, stage B: three patients and stage C: one patient). None of the samples from stages D and E generated clusters after culture, suggesting the absence of viable cancer cells after IOCS processing.

Conclusions

The salvaged blood may contain some tumour cells after processing with IOCS machine, but these cells are damaged and hence unable to replicate and unlikely to metastasise. The results of this study support the hypothesis that salvaged blood in MSTS is safe for transfusion.

Keywords

Metastatic spine tumour surgery Intraoperative cell salvage Leucocyte depletion filter Circulating tumour cells (CTCs) Microwells 

Notes

Acknowledgments

The authors thank AOSpine for granting AOSpine East Asia Research Award 2015 [AOSEA(R)2015-03]. 2. National University Health System for granting Bridging grant 2014 (NUHSRO/2014/013/Bridging/09) for conducting this study.

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Naresh Kumar
    • 1
  • Aye Sandar Zaw
    • 1
  • Bee Luan Khoo
    • 2
  • Sayantani Nandi
    • 2
  • Zhangxing Lai
    • 3
  • Gurpal Singh
    • 1
  • Chwee Teck Lim
    • 3
  • Jean Paul Thiery
    • 4
  1. 1.Department of Orthopaedic SurgeryUniversity Orthopaedics, Hand and Reconstructive MicrosurgerySingaporeSingapore
  2. 2.BioSystems and Micromechanics (BioSyM) Inter-Disciplinary Research Group, Singapore MIT Alliance for Research and Technology (SMART)National University of SingaporeSingaporeSingapore
  3. 3.Department of Biomedical EngineeringNational University of SingaporeSingaporeSingapore
  4. 4.Department of Biochemistry, Yong Loo Lin School of MedicineNational University of SingaporeSingaporeSingapore

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