Comparison of the healing status in suture-less end-to-end intestinal anastomosis using TachoSil with classic two-layer anastomosis in rats
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The classical two-layer anastomosis has been experimentally compared with anastomosis using synthetic TachoSil in terms of pre- and post-surgical symptoms and healing status of the anastomotic area in rats. Thirty-six adult male rats were randomly allocated into two groups: in group C (control), classic anastomosis was performed in the ileum of the small intestine, 10 cm away from the ileocecal valve using Vicryl 5-0 interrupted suture; group S (study) received anastomosis in the same area but with TachoSil using three sutures (mesenteric, anti-mesenteric, and anterior wall as suture-less method). The obtained results were analyzed by SPSS20 software, using Chi-square test. Based on the obtained results, group C had more favorable anastomotic healing than group S. Actually, the fibroblast and collagen were seen in most cases of the control rats, while healing was stopped in group S (TachoSil group) in early stages due to thrombosis and vasculitis gangrene. According to the findings, TachoSil patch cannot be advised for safe use in suturing the less small-bowel anastomosis.
KeywordsAnastomosis Experimental trial TachoSil Rat Peristalsis Anastomotic leakage Dilation
Research council of Kerman University of Medical sciences is greatly appreciated for supporting this project. The authors would like to thank Shiraz University of Medical Sciences, Shiraz, Iran and also Center for Development of Clinical Research of Nemazee Hospital and Dr. Nasrin Shokrpour for editorial assistance.
Compliance with ethical standards
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Conflict of interest
The authors declare that they have no conflict of interest.
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