Comparative Clinical Pathology

, Volume 29, Issue 1, pp 275–281 | Cite as

Haemoprofile of yellow-bellied house gecko, Hemidactylus flaviviridis Ruppell, 1835

  • Sarbeswar NayakEmail author
  • Prafulla Kumar Mohanty
Original Article


The study is carried out to investigate the haemogram of yellow-bellied house gecko in Odisha, India. Fifteen adult lizards of each sex were collected from the coastal area of Rajnagar block of Kendrapara, 754 225, Odisha, located in 20° 20′ N to 20° 37′ N latitude and 86° 14′ E to 87° 01′ E longitude. One millilitre of blood was collected from the ventral tail vein and transferred into vials containing ethylenediaminetetraacetic acid (EDTA) and then transported in icebox to laboratory. The morphology of blood cells along with haematological parameters like haemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), total leucocyte count (TLC), total platelet count (TPC), and differential leucocyte count (DLC) was calculated using standard procedures. Statistical analyses like correlation and t test were done. The study revealed that the TLC shows significant difference (t0.01) while the percentage of eosinophils is found to be significant at t0.05 between both sexes of lizard. The mean values of all other parameters are also showing difference between males and females of Hemidactylus flaviviridis Ruppell, 1835. The correlation with R2 values varies in males and females with respect to the parameters analysed. Some parameters are positively correlated with each other, and others are found to be negatively correlated. The data obtained could be useful in knowing the difference of haematological parameters between sexes of Hemidactylus flaviviridis.


Hemidactylus flaviviridis Sexes Haemogram Correlation Significant difference 



The authors express their gratefulness to Postgraduate Department of Zoology, Vikram Deb Autonomous College, Jeypore, Koraput, 764 001, for support and Postgraduate Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar, 751 004, for providing laboratory facilities.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The investigation followed all the guidelines and care of animals.


  1. Acharya G, Mohanty PK (2018) Effect of sex on haemocytobiochemical profiling of silver tigerfish Datnioides polota Hamilton, 1822. Comp Clin Pathol 27(5):1335–1342CrossRefGoogle Scholar
  2. Bailey KC, Smith SA, Zimmerman K, Lane R, Raskin RE, DeNardo D (2011) Hematology, leukocyte cytochemical analysis, plasma biochemistry, and plasma electrophoresis of wild-caught and captive-bred Gila monsters (Heloderma suspectum). Vet Clin Pathol 40(3):316–323CrossRefGoogle Scholar
  3. Bassert JM, Samples OM, Beal AD (2017) McCurnin’s clinical textbook for veterinary technicians, 9th edn. Elsevier- Health Science Division, Saunders, Philadelphia, pp 1–456Google Scholar
  4. Behera Y, Nayak S, Mohanty PK (2017) Age and season wise haematological profile of little egret (Egretta garzetta) of Chilika wetland. India. Asian J Anim Sci 11(4):158–164CrossRefGoogle Scholar
  5. Boudreaux MA (2010) Platelet structure. Chapter 75. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology. Blackwell Publishing Limited, Iowa, pp 561–568Google Scholar
  6. Campbell TW, Smith S, Zimmerman L (2010) Haematology of waterfowls and raptors. In: Weiss DJ, Wardrpo KJ (eds) 6th ednSchalm’s Vet Hematol. Wiley-Blackwell Publication, New Jersy, pp 977–986Google Scholar
  7. Chansue N, Sailasuta A, Tangtrongpiros J, Wangnaitha S, Assawawongkasem N (2011) Hematology and clinical chemistry of adult yellow-headed temple turtles (Hieremys annandalii). Vet Clin Pathol 40:174–184CrossRefGoogle Scholar
  8. Cheville NF (2009) Ultrastructural pathology: the comparative cellular basis of disease, 2nd edn. Wiley-Blackwell Publication, Ames, Iowa, pp 1–1000CrossRefGoogle Scholar
  9. Colville T, Bassert JM (2015) Laboratory manual for clinical anatomy and physiology for veterinary technicians, 3rd edn. Mosby, St. Louis, Missouri pp 1–656Google Scholar
  10. Das I (2010) A field guide to the reptiles of Southeast Asia. New Holland Publishers (UK), Ltd, London, pp 1–56Google Scholar
  11. Das I, Das A (2017) A naturalist’s guide to the reptiles of India, Bangladesh, Bhutan, Nepal, Pakistan and Sri Lanka. John Beaufoy Publishing Limited, Woodstock Road, Oxford, England, pp 1–176Google Scholar
  12. Gul C, Tosunoglu M (2011) Haematological reference intervals of four agamid lizard species from Turkey (Squamata: Sauria: Agamidae). Herpetozoa 24(1/2):51–59Google Scholar
  13. Jensen AL, Kjelgaard-Hansen M (2010) Diagnostic test validation. Chapter 130. In: Weiss DJ, Wardro KJ (eds) Schalm’s veterinary hematology. Blackwell Publishing Limited, Iowa, pp 1027–1033Google Scholar
  14. Lillie RD (1977) Methods for testing biological stains. In: Conn’s HJ biological stains, 9th edn. The Williams and Wilkins Company, Baltimore, USA, pp 606–607Google Scholar
  15. Nayak S, Mohanty PK (2018) Haematological analysis of leschenault’s leaf toad gecko, Hemidactylus leschenaultii Dumeril and Bibron, 1836. Indian J Biol 5(1):53–59Google Scholar
  16. Olayemi OA (2011) Hematological parameters of house gecko (Hemidactylus frenatus) in Ibadan Metropolis, Nigeria. Medwell J Vet Res 4(3):77–80Google Scholar
  17. Omonona A, Olukole SG, Fushe FA (2011) Haematology and serum biochemical parameters in free ranging African side neck turtle (Pelusios sinuatus) in Ibadan, Nigeria. Acta Herpetol 6(2):267–274Google Scholar
  18. Pal A, Parida SP, Swain MM (2008) Hematological and plasma biochemistry in fan-throated lizard, Sitana ponticeriana (Sauria: Agamidae). Rus J Herp Rus 15(2):110–116Google Scholar
  19. Parida SP, Dutta SK, Pal A (2012) Hematological and plasma biochemistry in Psammophilus blanfordanus (Sauria: Agamidae). Comp Clin Pathol 21:1387–1394CrossRefGoogle Scholar
  20. Parida SP, Dutta SK, Pal A (2013) Hematology and plasma chemistry of wild keeled Indian Mabuya, Eutropis carinata (Schneider 1801). Comp Clin Pathol 22:869–873CrossRefGoogle Scholar
  21. Parida SP, Dutta SK, Pal A (2014) Hematology and plasma biochemistry of wild-caught Indian cobra Naja naja (Linnaeus, 1758). J Venomous Anim Toxins Incl Trop Dis 20:14CrossRefGoogle Scholar
  22. Ponsen S, Talabmook C, Narkkong N, Aengwanich W (2008) Blood cell characteristics and some hematological values of sand lizards (Leiolepis belliana rubritaeniata Mertens 1961) of Northeastern Thailand. Int J Zool Res 4(2):119–123CrossRefGoogle Scholar
  23. Reagan WJ, Rovira ARI, DeNicola DB (2008) Reptilian hematology. Chapter 12, in: veterinary hematology: atlas of common domestic and non-domestic species. Wiley-Blackwell, Iowa, pp 85–95Google Scholar
  24. Rizzi TE, Meinkoth JH, Clinkenbeard KD (2010) Normal hematology of the dog, chapter 104. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology. Blackwell Publishing Incorporated, Iowa, p 804Google Scholar
  25. Rossini M, Garcia G, Rojas J, Zerpa H (2011) Hematologic and serum biochemical reference values for the wild spectacled Caiman, Caiman crocodilus crocodilus, from the Venezuelan plains. Vet Clin Pathol 40:374–379CrossRefGoogle Scholar
  26. Rovira ARI (2010) Hematology of reptiles. Chapter 127. In: Weiss DJ, Wardrop KJ (eds) Schalm’s veterinary hematology. Blackwell Publishing Limited, Iowa, pp 1004–1012Google Scholar
  27. Saggese M (2009) Clinical approach to the anaemic reptile. J Exotic Pet Med 18:98–111CrossRefGoogle Scholar
  28. Smyth AK, Smee E, Godfrey SS, Crowther M, Phalen D (2017) The use of body condition and haematology to detect widespread threatening processes in sleepy lizards (Tiliqua rugosa) in two agricultural environments. R Soc Open Sci 1:1–13Google Scholar
  29. Stacy NI, Alleman AR, Sayler KA (2011) Diagnostic hematology of reptiles. Clin Lab Med 31:87–108CrossRefGoogle Scholar
  30. Sykes JM, Klaphake E (2008) Reptile hematology. Vet Clin North Am Exot Anim Pract 11:481–500CrossRefGoogle Scholar
  31. Thrall MA, Weiser G, Allison R, Campbell TW (2012) Veterinary hematology and clinical chemistry. John Wiley and Sons, New Jersey, pp 237–273Google Scholar
  32. Troiano JC, Gould EG, Gould I (2008) Hematological reference intervals in Argentina lizard Tupinambis merianae (Sauria-Teiidae). Comp Clin Pathol 17:93–97CrossRefGoogle Scholar
  33. Vasaruchapong T, Disarapong P, Chulasugandha P, Khow O, Chanhome L, Chiobamroongkiat M, Chaiyabutr N, Sitprija V (2014) Comparative studies on hematological and plasma biochemical parameters in different types of venomous snakes in Thailand. Comp Clin Pathol 23(4):955–959. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of ZoologyVikram Deb Autonomous CollegeKoraputIndia
  2. 2.Department of ZoologyUtkal University, Vani ViharBhubaneswarIndia

Personalised recommendations