Comparative Clinical Pathology

, Volume 29, Issue 1, pp 155–160 | Cite as

Evaluation of time and temperature storage in platelet counts in blood samples of dogs

  • Antonise Mariely JaguezeskiEmail author
  • Julieta Volpato
  • Carla Dezan de Lorenzi Cancelier
  • Mariângela Lovatel
  • Ádson Costa
  • Nádia Cristine Weinert
  • Suzane Lillian Beier
  • Cláudio Roberto Scabelo Mattoso
  • Mere Erika Saito
Original Article


The reliability of platelet counts obtained by different methods following different storage times/conditions is not well defined. The aim was to evaluate temporal changes in platelet count of canine blood samples submitted to two thermal storage conditions using three techniques of platelet counting. Blood samples were obtained from 40 dogs aged between 6 months and 12 years. Blood samples were collected by jugular venipuncture, separated into two aliquots, and stored in EDTA tubes: one kept at room temperature (RT group) and the other kept under refrigeration (RE group). The platelet count was performed using the hemocytometer method, blood smear counting, and automated counting immediately after blood collection (M0) and 30 min and 1, 2, 4, 6, and 24 h later in both groups. The platelet parameters provided by the electronic counter were also obtained. There was a significant difference in platelet counts between the manual and automated methods when the samples were processed 24 h after sample collection. The refrigerated samples remained with closer platelet values of the previous samples for longer than those stored at room temperature. The analysis of platelet parameters loses its validity when the samples are stored for more than 6 h. The plaquetogram is reliable when the sample is processed up to 4 h after collection, regardless of the storage method. The timing of the platelet count should not exceed 6 h after collection for refrigerated samples and 4 h for samples stored at room temperature, for all counting methods.


Automated counting Hemocytometer Plaquetogram Storage times 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for and use of animals were followed.


  1. Abella NHG, Geffré A, Deshuillers PL et al (2014) Changes in hematology measurements in healthy and diseased dog blood stored at room temperature for 24 and 48 hours using the XT-2000iV analyzer. Vet Clin Pathol 43:24–35CrossRefGoogle Scholar
  2. Clark P, Mogg TD, Tvedten HW, Korcal D (2002) Artifactual changes in equine blood following storage, detected using the Advia 120 hematology analyzer. Vet Clin Pathol 31:90–94CrossRefGoogle Scholar
  3. Coelho E (1981) Técnicas de estudo da coagulação, 3rd edn. Santos, São Paulo, p 121Google Scholar
  4. Comar SR, Silva PH (2009) Determinação laboratorial e aplicação clínica dos parâmetros de volume plaquetário. RBAC 41(1):257–265Google Scholar
  5. Farias MG, Bó SD (2008) Determinação do intervalo de referência para o volume plaquetário médio (VPM) utilizando o analisador hematológico Pentra 120 ABX. RBAC 40(1):39–41Google Scholar
  6. Ferreira GS, Masson GCIH, Costa EDCP et al (2009) Plateletcrit, mean platelet volume and platelet distribution width: its expected values and correlation parameters in dogs from northern region of Brazil. In: World Small Animal Veterinary Association World Congress Proceedings. Universidade Federal Rural da Amazônia-UFRA, BelémGoogle Scholar
  7. Gao XM, Moore XL, Liu Y, Wang XY, Han LP, Su Y, Tsai A, Xu Q, Zhang M, Lambert GW, Kiriazis H, Gao W, Dart AM, du XJ (2016) Splenic release of platelets contributes to increased circulating platelet size and inflammation after myocardial infarction. Clin Sci 130:1089–1104CrossRefGoogle Scholar
  8. Gerday E, Baer VL, Lambert DK (2009) Testing platelet mass versus platelet count to guide platelet transfusions in the neonatal intensive care unit. Transfusion 49:2034–2039CrossRefGoogle Scholar
  9. Golwala ZM, Shah H, Gupta N, Sreenivas NGV, Puliyel JM (2016) Mean platelet volume (MPV), platelet distribution width (PDW), platelet count and plateletcrit (PCT) as predictors of in-hospital paediatric mortality: a case-control study. Afr Health Sci 16:356–362CrossRefGoogle Scholar
  10. Gulcu E, Durmaz Y, Karabay O (2013) Effect of severe sepsis on platelet count and their indices. Afr Health Sci 13(2):333–338Google Scholar
  11. Humann-Ziehank E, Ganter M (2012) Pre-analytical factors affecting the results of laboratory blood analyses in farm animal veterinary diagnostics. Animal 6:1115–1123CrossRefGoogle Scholar
  12. Jackson SR, Carter JM (1993) Platelet volume: laboratory measurement and clinical application. Blood Rev 7:104–113CrossRefGoogle Scholar
  13. Jain NC (1986) The platelets: structural, biochemical and functional aspects. In: Schalm’s veterinary hematology, 4th edn. Lea & Febiger, Philadelphia, pp 1–4Google Scholar
  14. Knoll JS, Rowell S (1996) Clinical hematology. In-clinic analysis, quality control, reference values and system selection. Veterinary Clinics of North America: Small Animal Practice, Philadelphia, pp 981–1002Google Scholar
  15. Lassen ED, Weisner G (2007) Tecnologia Laboratorial em Medicina Veterinária. In: Thrall MA (ed) Hematology and veterinary clinical biochemistry. São Paulo, Editora Roca, pp 3–36Google Scholar
  16. Matos JF, Carvalho MG, Dusse LMS et al (2008) O papel do RDW, da morfologia eritrocitária e de parâmetros plaquetários na diferenciação entre anemias microcíticas e hipocrômicas. Rev Bras Hematol Hemoter 30:463–469Google Scholar
  17. Médaille C, Briend-Marchal A, Braun JP (2002) Stability of selected hematology variables in canine blood kept at room temperature in EDTA for 24 and 48 hours. Vet Clin Pathol 35:18–23CrossRefGoogle Scholar
  18. Moreno A, Menke D (2002) Assessment of platelet numbers and morphology in the peripheral blood smear. Clin Lab Med 22:193–213CrossRefGoogle Scholar
  19. Norman EJ, Barron RC, Nash AS (2001) Prevalence of low automated platelet counts in cats: comparison with prevalence of thrombocytopenia based on blood smear estimation. Vet Clin Pathol 30:137–140CrossRefGoogle Scholar
  20. Olsen LH, Kristensen AT, Qvortrup K, Pedersen HD (2004) Comparison of manual and automated methods for determining platelet count in dogs with macrothombocitopenia. J Vet Diagn Investig 16:167–170CrossRefGoogle Scholar
  21. Pastor J, Cuenca R, Velarde R, Viñas L, Lavin S (1997) Evaluation of a hematology analyzer with canine and feline blood. Vet Clin Pathol 26:138–147CrossRefGoogle Scholar
  22. Prins MW, Van Leeuwen MW, Teske E (2009) Stability and reproducibility of advia 120-measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin contente (CHr) in the diagnosis of iron deficiency. Tijdschr Diergeneeskd 134:272–278PubMedGoogle Scholar
  23. Reardom DM, Hutchinson D, Preston FE, Trowbridge EA (1985) The routine measurement of platelet volume: a comparison of aperture impedance and flow cytometric systems. Clin Lab Haematol 7:251–257CrossRefGoogle Scholar
  24. Schäfers A, Meierhans S, Sauter-Louis C et al (2013) Reference values for haematological and clinical-chemical parameters in the dog. Tierarztl Prax Ausg K 41(3):163–172Google Scholar
  25. Schwartz D, Sharkey L, Armstrong PJ, Knudson C, Kelley J (2014) Platelet volume and plateletcrit in dogs with presumed primary immune-mediated thrombocytopenia. J Vet Intern Med 28:1575–1579CrossRefGoogle Scholar
  26. Schweirgert A, Rezende FH, Fantoni DT, Moroz LR (2010) Avaliação da contagem plaquetária pelo contador automático QBC Vet Autoread® comparado com estimativa em esfregaço sanguíneo e contagem em hemocitômetro. Semin Cienc Agrar 31:1001–1008CrossRefGoogle Scholar
  27. Shi G, Morrell CN (2011) Platelets as initiators and mediators of inflammation at the vessel wall. Thromb Res 127:387–390CrossRefGoogle Scholar
  28. Smith JR, Smith KF, Brainard BM (2014) Platelet parameters from an automated hematology analyzer in dogs with inflammatory clinical diseases. Vet J 201:406–411CrossRefGoogle Scholar
  29. Souza AM, Pereira JJ, Campos SDE et al (2016) Platelet indices in dogs with thrombocytopenia and dogs with normal platelet counts. Arch Med Vet 48:27–281CrossRefGoogle Scholar
  30. Stokol T, Erb HN (2007) A comparison of platelet parameters in EDTA and citrate anticoagulated blood in dogs. Vet Clin Pathol 36:148–154CrossRefGoogle Scholar
  31. Tasker S, Cripps PJ, Mackin AJ (2001) Evaluation of methods of platelet counting in the cat. J Small Anim Pract 42:326–332CrossRefGoogle Scholar
  32. Thompson CB, Jakubowski JA (1988) The pathophysiology and clinical relevance of platelet heterogeneity. Blood 72:1–8CrossRefGoogle Scholar
  33. Topper MJ, Weller EG (2003) Hemostasis. In: Latimer KS, Mahaffey EA, Prasse KW (eds) Duncan & Prasse’s veterinary laboratory medicine: clinical pathology, 4th edn, pp 99–135Google Scholar
  34. Yilmaz Z, Eralp O, Ilcol YO (2008) Evaluation of platelet count and its association with plateletcrit, mean platelet volume, and platelet size distribution width in a canine model of endotoxemia. Vet Clin Pathol 37:159–163CrossRefGoogle Scholar
  35. Zelmanovic D, Hetherington EJ (1998) Automated analysis of feline platelets in whole blood, including platelet count, mean platelet volume and activation state. Vet Clin Pathol 27:2–9CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Antonise Mariely Jaguezeski
    • 1
    Email author
  • Julieta Volpato
    • 2
  • Carla Dezan de Lorenzi Cancelier
    • 2
  • Mariângela Lovatel
    • 2
  • Ádson Costa
    • 2
  • Nádia Cristine Weinert
    • 2
  • Suzane Lillian Beier
    • 3
  • Cláudio Roberto Scabelo Mattoso
    • 3
  • Mere Erika Saito
    • 2
  1. 1.Department of Toxicological BiochemistryUniversidade Federal de Santa Maria – UFSMSanta MariaBrazil
  2. 2.Department of Veterinary MedicineUniversidade do Estado de Santa Catarina (UDESC)LagesBrazil
  3. 3.Department of Veterinary Clinic and SurgeryUniversidade Federal de Minas Gerais (UFMG)Belo HorizonteBrazil

Personalised recommendations