Comparative Clinical Pathology

, Volume 29, Issue 1, pp 69–73 | Cite as

Haematology, plasma biochemistry and serum of table size African catfish, Clarias gariepinus, naturally infected with Listeria species in Oyo State

  • Ibrahim AdeshinaEmail author
  • Benjamin O. Emikpe
  • Adetola Jenyo-Oni
  • Emmanuel K. Ajani
  • Musa I. Abubakar
Original Article


Fresh fish in Nigeria is gaining acceptance as a result of knowledge of its nutritive value; however, it is mostly assessed by the consumers by mere looking. Fish that looks apparently healthy may not be actually healthy. Haematology, plasma biochemistry and serum are some good parameters for the assessment of fish health. This study evaluated the haematology, plasma biochemistry and blood enzymes of farm-raised African catfish, Clarias gariepinus, naturally infected with listeriosis in Oyo State. Fish were collected from 36 farms using stratified method. A total of 108 fish were collected where 37 were positive for listeriosis. The blood of the affected fish was used for this study. Haematology, plasma biochemistry and blood enzyme analysis were determined. Fish from Oyo zone had the highest PCV, Hb, RBC and MCHC with 29.33 ± 2.96%, 10.04 ± 1.04 dL, 3.55 ± 0.09 × 106/μL and 34.24 ± 0.74%, respectively; the highest heterocytes (39.00 ± 5.33%), eosinophils (4.00 ± 1.10%), basophils (0.50 ± 0.55%) and hetero:lymphocyte ratio (0.71 ± 0.14) were recorded in fish from Ogbomosho zone; WBC, platelets, lymphocytes and monocytes were highest in fish from Saki zone with 18.72 ± 0.44 × 106/μL, 224.00 ± 3.61 × 103/μL, 71.33 ± 1.53% and 3.33 ± 0.58%, respectively, while fish from Ibadan/Ibarapa zone had the highest MCV (163.40 ± 44.23 fL) and MCH (52.77 ± 14.95 pg). Total protein and globulin were highest in fish from Oyo zone with 6.61 ± 0.37 and 1.70 ± 0.38 g/dL, respectively, while albumin and A:G ratio were highest in fish from Ogbomosho zone. Fish from Ogbomosho zone had the highest AST, ALP, creatinine and glucose with 194.17 ± 10.11 μL, 232.17 ± 63.64 μL, 0.72 ± 0.19 μL and 277.50 ± 90.36 mg/dL, respectively; urea and cholesterol were highest in fish from Saki zone (8.30 ± 0.46 μL and 263.67 ± 3.51 mg/dL) while Ibadan/Ibarapa zone had the highest ALT (37.11 ± 14.72 μL). The study concluded that fish that are physically healthy which is the common factor used to judge the health status of the fish by majority of consumers do not mean that the fish are healthy. Haematology, biochemistry and blood enzymes of the fish infected with listeriosis are lower than the recommended levels. Therefore, further research should be carried out to detect a quicker way of examining healthy fish.


Haematology Serum Plasma biochemistry Listeria species Infection 



The project was partly funded by University of Ilorin, Ilorin, under staff development fund (SDA/2015).

Compliance with ethical standards

The protocol of the study was subjected to ethical consideration and was approved by Animal Care Use and Research Ethics Committee, University of Ibadan, Ibadan, Oyo State, Nigeria, with reference number UI-ACUREC/App/03/2017/008.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abdel-Tawwab M, Adeshina I, Jenyo-Oni A, Ajani EK, Emikpe BO (2018) Growth, physiological, antioxidants, and immune response of African catfish, Clarias gariepinus (B.), to dietary clove basil, Ocimum gratissimum, leaf extract and its susceptibility to Listeria monocytogenes infection. Fish Shellfish Immun 78:346–354. CrossRefGoogle Scholar
  2. Adeshina I, Abdulwahab M, Adewale YA, Suleiman SB, Tiamiyu LO (2017) Detection of Listeria monocytogenes in fried fish, processing slab and tools in Kwara State, Nigeria, Harran J Fac Vet Med 6(1):1–8Google Scholar
  3. Adeshina I, Emikpe BO, Jenyo-Oni A, Ajani EK (2018a) Distribution and factors associated with occurrence of Listeria monocytogenes in table size African catfish, pond water and sediment in two ecological zones in Nigeria. J Appl Biol Sci 12(3):14–20Google Scholar
  4. Adeshina I, Emikpe BO, Jenyo-Oni A, Ajani EK, Abdel-Tawwab M (2018b) Stimulatory effect of dietary clove, Eugenia caryophyllata bud extract on growth performance, nutrient utilization, antioxidant capacity, and tolerance of African catfish, Clarias gariepinus B., to Aeromonas hydrophila. J World Aquac Soc 49:6):1–6)16. CrossRefGoogle Scholar
  5. Adeshina I, Emikpe BO, Jenyo-Oni A, Ajani EK, Abubakar MI (2019) Growth performance, gut morphometry and innate immune profiles of common carp, Cyprinus carpio juveniles fed diet fortified with Mitracarpus scaber leaves extract and its susceptibility to pathogenic bacteria, Aeromonas hydrophila, Acta Biolog 26:32–39.
  6. Adeshina I, Jenyo-Oni A, Ajani EK, Adewale YA (2016) Natural occurrence of Diplostomum Spp. in farm raised African catfish (ClariasGariepinus) in Oyo State Intl J Vet Sci Med 4(2):41–45. CrossRefGoogle Scholar
  7. Agbede SA, Ogunsanmim AO, Taiwo VO, Ogundipe TI (1999) Toxic effects of poultry faeces on Clarias gariepinus broodstock. Trop Vet 17:18–21Google Scholar
  8. Ajeniyi SA, Solomon RJ (2014) Urea and creatinine of Clarias gariepinus in three different commercial ponds. Nat Sci 12(10):124–138Google Scholar
  9. Chukwu OOC (2007) Studies of Listeria monocytogenes (bacterium) in some selected local government areas of plateau state, Nigeria, Ph.D Thesis submitted to Department of Botany, University of Jos, 256Google Scholar
  10. Fagbenro OA, Adebayo OT (2005) A review of the animal and aquafeed in Nigeria. In a synthesis of the formulated animal and aquafeed industry in sub-Sahara Africa. CIFA Occasional 26(2):36Google Scholar
  11. Fagbenro OA, Adedire CO, Owoseeni EA, Ayotunde EO (1993) Studies on the biology and aquaculture potential of feral catfish, Heterobranchus bidorsalis (Geofferoyst.Hilaire, 1809) clarridae. Trop Zool 6:77–79CrossRefGoogle Scholar
  12. Fagbenro AO, Adeparusi E (2003) Feedstuff and dietary substitution for farmed fish in Nigeria. Paper presented at Pan African Fish and Fisheries Conference Contonou, Benin Republic. 276.Google Scholar
  13. Fagbenro, OA (2005): Aquaculture in Nigeria: history, status and prospects. A report of FAO World Fish Centre Workshop, CameroonGoogle Scholar
  14. Ferreira ML, Avenant-Oldewage A (2013) Notes on the occurrence of Trypanosoma sp. (Kinetoplastida: Trypanosomatidae) in freshwater fishes from South Africa. Onderstepoort J Vet Res 80.
  15. Miettinen H (2006) Listeria monocytogenes if fish farming and processing, PhD Thesis, University of Helsinki, Finland, 77Google Scholar
  16. National Kidney Foundation (2002) Clinical practice guidelines for chronic kidney disease: evaluation, classification and stratification. Am J. Kidney Dis, 39: 1–266Google Scholar
  17. Oguntade OR,Ayinla OA, Oresegun A, Adeogun OA, Ogunbadejo HK, Alhaji T (2006) Trends in fish culture in urban and periurban areas of Lagos State. In: E.J. Ansa, P.E. Anyanwu, B.W. Ayonadu, E.S. Erondu & S.N. Deekae, eds. Proceedings of 20th Fisheries Society of Nigeria Annual Conf., PortHarcourt, 14th – 18th November, 2005. 56–67Google Scholar
  18. Oyelese AO, Taiwo VO, Ogunsanmi AO, Faturoti EO (1999) Toxicological effects of cassava peels on haematology serum biochemistry and tissue pathology of Clarias gariepinus fingerlings. Trop Vet 17:17–30Google Scholar
  19. Oyo State Government (OYSG) (2015) Official Website of Oyo State Government. Available on accessed on 1/6/2015 9:59pm
  20. Salihu MD, Junaidu AU, Manga SB, Gulumbe ML, Magaji AA, Ahmed A, Adamu AY, Shittu A, Balarabe I (2008) Occurrence of Listeria monocytogenes in smoked fish in Sokoto, Nigeria. J Biotech 7(17):3082–3084Google Scholar
  21. Soivio A, Oikari A (1976) Haematological effects of stress on a teleost, Esoxlucius L. J Fish Biol 8:397–411CrossRefGoogle Scholar
  22. Witting MD, Magder L, Heins E, Mattu A, Granja CA, Baumgarten M (2006) ED predictors of upper gastrointestinal tract bleeding in patients without hematemesis. Am J Emerg Med 24(3):280–285 Available on CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Aquaculture and FisheriesUniversity of IlorinIlorinNigeria
  2. 2.Department of Veterinary PathologyUniversity of IbadanIbadanNigeria
  3. 3.Department of Aquaculture and Fisheries ManagementUniversity of IbadanIbadanNigeria

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