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Comparative Clinical Pathology

, Volume 29, Issue 1, pp 83–93 | Cite as

Clinical blood gas indices and histopathological effects of intrathecal injection of tolfenamic acid and lidocaine Hcl in donkeys

  • Mohammed A. H. Abdelhakiem
  • Abdelbaset Eweda AbdelbasetEmail author
  • Mahmoud Abd-Elkareem
  • Mohamed S. Rawy
  • Hamdy F. F. Mahmoud
Original Article
  • 60 Downloads

Abstract

The present study aimed to investigate the clinical blood gas indices and histopathological consequences after intrathecal injection of tolfenamic acid and lidocaine Hcl and moreover, to elucidate the spinal safety of tolfenamic acid as a cyclooxygenase inhibitor in donkeys. Ten clinically healthy donkeys were divided into two groups, 5 animals each. The first group received lidocaine Hcl 2% and the second one received tolfenamic acid 4% intrathecally. The physical parameters and ataxia, analgesia, and motor blockade scores were recorded. Blood gases and acid base balance indices and histopathological examination were done. Blood pH level was significantly decreased (P < 0.05) and the blood pCO2 level was significantly increased (P < 0.05) 15 min after intrathecal injection of tolfenamic acid. Additionally, there was a significant difference in the motor block scores between the two groups at 2 and 4 h post-injection. Histopathological findings of the spinal cord of tolfenamic acid–injected group revealed neurodegeneration and necrosis which were manifested clinically by paraplegia. In conclusion, the present study uncovered the analgesic and motor effects of commercially prepared tolfenamic acid following intrathecal injection in donkeys. Nevertheless, it is unsafe because of its neurotoxic effect on the spinal cord which was manifested clinically by paraplegia of donkeys. On the other hand, intrathecal administration of lidocaine Hcl was safe and causes nonserious cardiopulmonary changes.

Keywords

Analgesia Blood gases Intrathecal Tolfenamic acid Lidocaine Hcl Donkeys 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

The experimental protocol of the current study was approved by the Animal Care and Ethics Committee of Assiut University, Assiut, Egypt.

References

  1. Aygün D, Kaplan S, Odaci E, Onger ME, Altunkaynak ME (2012) Toxicity of non-steroidal anti- inflammatory drugs: a review of melatonin and diclofenac sodium association. Histol Histopathol 27:417–436PubMedGoogle Scholar
  2. Brosnan RJ, Steffey EP, LeCouteur RA, Imai A, Farver TB, Kortz GD (2002) Effects of body position on intracranial and cerebral perfusion pressures in isoflurane-anesthetized horses. J Appl Physiol 92:2542–2546CrossRefGoogle Scholar
  3. Buckenmaier C, Nielsen KC, Pietrobon R, Klein SM, Martin AH, Greengrass RA, Steele SM (2002) Small-dose intrathecal lidocaine versus ropivacaine for anorectal surgery in an ambulatory setting. Anesth Analg 95(5):1253–1257CrossRefGoogle Scholar
  4. Budras K, Sack W, Röck R, Horowitz A, Berg R (2009) Anatomy of the horse. 5th ed., Schlütersche Verlagsgesellschaft mbH & Co. KG., Hans-Böckler-Alle 7, 30173 Hannover, GermanyGoogle Scholar
  5. DeRossi R, Módolo T, Pagliosa R, Jardim P, Maciel F, Macedo GG (2012) Comparison of analgesic effects of caudal epidural 0.25% bupivacaine with bupivacaine plus morphine or bupivacaine plus ketamine for analgesia in conscious horses. J Equine Vet Sci 32:190–195Google Scholar
  6. Devoghel JC (1993) Intrathecal injection of lysine-acetylsalicylate in man with intractable cancer pain. In Progress in pharmacology and clinical pharmacology (Jurna I, Yaksh TL, Eds.); Vol. 10, pp. 111–118. Gustav Fischers VerlagGoogle Scholar
  7. Dolan S, Nolan AM (2000) Behavioural evidence supporting a differential role for group I and II metabotropic glutamate receptors in spinal nociceptive transmission. Neuropharmacology 39:1132–1138CrossRefGoogle Scholar
  8. Duncanson G (2012) Veterinary treatment of sheep and goats. 1st ed. MPG Books Group, UKCrossRefGoogle Scholar
  9. Fischer AH, Jacobson KA, Rose J, Zeller R (2008) Hematoxylin and eosin staining of tissue and cell sections. CSH ProtocGoogle Scholar
  10. Furr M, Reed S (2008) Equine neurology. Blackwell Publishing LtdGoogle Scholar
  11. Greene SA (2002) Veterinary anesthesia and pain management secrets (. Ed.) 1st edition. HANLEY & BELFUS, INC. Medical Publishers 210 South 13th Street Philadelphia, PA 19107Google Scholar
  12. Hanson PD, Maddison JE (2008) Nonsteroidal anti-inflammatory drugs and chondroprotective agents. In. Small animal clinical pharmacology (Maddison, J. Editor) 2nd ed. Saunders, Elsevier. London; 287–307Google Scholar
  13. Hayta E, Elden H (2018) Acute spinal cord injury: a review of pathophysiology and potential of non-steroidal antiinflammatory drugs for pharmacological intervention. J Chem Neuroanat 87:25–31CrossRefGoogle Scholar
  14. Jaussaud P, Guieu D, Bellon C, Barbier B, Lhopital MC, Sechet R, Courtot D, Toutain PL (1992) Pharmacokinetics of tolfenamic acid in the horse. Equine Vet J Suppl (11):69–72Google Scholar
  15. Jittreetat T, Shin YS, Hwang HS, Lee BS, Kim YS, Sannikorn P, Kim CH (2016) Tolfenamic acid inhibits the proliferation, migration, and invasion of nasopharyngeal carcinoma: involvement of p38-mediated down-regulation of slug. Yonsei Med J 57(3):588–598CrossRefGoogle Scholar
  16. Kang SU, Shin YS, Hwang HS, Baek SJ, Lee SH, Kim CH (2012) Tolfenamic acid induces apoptosis and growth inhibition in head and neck cancer: involvement of NAG-1 expression. PLoS One 7(4):e34988CrossRefGoogle Scholar
  17. Kasibhatla S, Amarante-Mendes GP, Finucane D, Brunner T, Bossy-Wetzel E, Green DR (2006) Acridine Orange/ethidium bromide (AO/EB) staining to detect apoptosis. CSH ProtocGoogle Scholar
  18. Lees P (2009) Analgesic, antiinflammatory, antipyretic drugs. In.Veterinary pharmacology and therapeutics (Riviere, J; Papich, M. and Adams, H.R. Editors). Ninth ed. Willey-Blackwell. USA; 457–492Google Scholar
  19. Lees P (2018) Analgesic, antiinflammatory, antipyretic drugs. In. (Veterinary pharmacology and therapeutics Riviere, J.E and Papich, M.G. Eds) tenth edition. Wiley Blackwell. PP. 467–500Google Scholar
  20. Lefebvre HP, Laroute V, Alvinerie M, Schneider M, Vinclair P, Braun JP, Toutain PL (1997) The effect of experimental renal failure on tolfenamic acid disposition in the dog. Biopharm Drug Dispos 18(1):79–91CrossRefGoogle Scholar
  21. Malmberg AB, Yaksh TL (1992) Antinociceptive actions of spinal nonsteroidal anti-inflammatory agents on the formalin test in the rat. J Pharmacol Exp Ther 263:136–146PubMedGoogle Scholar
  22. Muir W (2009) Anxiolytics, Nonopioid sedative-analgesics, and opioid analgesics. In. Equine anesthesia: monitoring and emergency therapy (Muir, W and Hubbell, J. Eds), 2nd ed. Saunders, Elsevier, USA; 185–209Google Scholar
  23. Plumb D (2011) Plumb’s veterinary drug handbook. 7th ed. PharmaVet Inc., StockholmGoogle Scholar
  24. Robinson EP, Natalini CC (2002) Epidural anesthesia and analgesia in horses. Vet Clin North Am (Equine Pract) 18(1):61–82CrossRefGoogle Scholar
  25. Samad TA, Moore KA, Sapirstein A, Billet S, Allchorne A, Poole S, Bonventre JV, Woolf CJ (2001) Interleukin-1β-mediated induction of cox-2 in the CNS contributes to inflammatory pain hypersensitivity. Nature 410:471–475CrossRefGoogle Scholar
  26. Sams RA, Muir W (2009) Principles of drug disposition and drug interaction in horse. In. Equine anesthesia: monitoring and emergency therapy. 2nd ed. SAUNDERS ELSEVIER. 11830 Westline industrial drive. St. Louis, Missouri 63146. PP. 171–184Google Scholar
  27. Sankpal UT, Lee CM, Connelly SF, Kayaleh O, Eslin D, Sutphin R, Goodison S, Adwan L, Zawia NH, Lichtenberger LM, Basha R (2013) Cellular and organismal toxicity of the anti-cancer small molecule, tolfenamic acid: a pre-clinical evaluation. Cell Physiol Biochem 32:675–686CrossRefGoogle Scholar
  28. Scott DB (1986) Toxic effects of local anaesthetic agents on the central nervous system. Br J Anaesth 58:732–735CrossRefGoogle Scholar
  29. Sidhu PK (2006) Pharmacokinetic and pharmacodynamic interactions of tolfenamic acid and marbofloxacin in goats. Res Vet Sci 80:79–90CrossRefGoogle Scholar
  30. Sidhu PK, Landoni MF, Lees P (2005) Influence of marbofloxacin on the pharmacokinetics and pharmacodynamics of tolfenamic acid in calves. J Vet Pharmacol Ther 28:109–119CrossRefGoogle Scholar
  31. Sidhu PK, Landoni MF, Lees P (2006) Pharmacokinetic and pharmacodynamic interactions of tolfenamic acid and marbofloxacin in goats. Res Vet Sci 80(1):79–90CrossRefGoogle Scholar
  32. Skarda RT, Muir WW, Ibrahim AL (1985) Spinal fluid concentrations of mepivacaine in horses and procaine in cows after thoracolumbar subarachnoid analgesia. Am J Vet Res 46:1020–1024PubMedGoogle Scholar
  33. Skarda RT, Muir W, Hubbell JAE (2009) Local anesthetic drugs and techniques. In. Equine anesthesia: monitoring and emergency therapy (Muir, W. and Hubbell J. Eds.) 2nd ed., Saunders, Elsevier. St. Louis, Missouri 63146. USA; 210–242CrossRefGoogle Scholar
  34. Staffieri F, Driessen B, Lacitignolas L, Crovace A (2009) A comparison of subarachnoid buprenorphine or xylazine as an adjunct to lidocaine for analgesia in goats. Vet Anaesth Analg 36:502–511CrossRefGoogle Scholar
  35. Svensson CI, Yaksh TL (2002) The spinal phospholipase-cyclooxygenase-prostanoid cascade in nociceptive processing. Annu Rev Pharmacol Toxicol 42:553–583CrossRefGoogle Scholar
  36. Taylor FGR, Brazil T, Hillyer MH (2010) Diagnostic techniques in equine medicine. 2nd edition. Saunders Elsevier; 303Google Scholar
  37. Yahia D, Abd El-Hakiem M (2014) Biochemical analysis of synovial fluid, cerebrospinal fluid and vitreous humor at early postmortem intervals in donkeys. J Adv Vet Res 4(1):6–11Google Scholar
  38. Yaksh TL, Horais KA, Tozier N, Rathbun M, Richter P, Rossi S, Grafe M, Tong C, Meschter C, Cline JM, Eisenach J (2004) Intrathecal ketorolac in dogs and rats. Toxicol Sci 80(2):322–334CrossRefGoogle Scholar
  39. Yamashita A, Matsumoto M, Matsumoto S, Itoh M, Kawai K, Sakabe T (2003) A comparison of the neurotoxic effects on the spinal cord of tetracaine, lidocaine, bupivacaine, and ropivacaine administered intrathecally in rabbits. Anesth Analg 97(2):512–519CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary MedicineAssiut UniversityAssiutEgypt
  2. 2.Animal Medicine Department (Clinical Laboratory diagnosis), Faculty of Veterinary MedicineAssiut UniversityAssiutEgypt
  3. 3.Department of Anatomy and Histology, Faculty of Veterinary MedicineAssiut UniversityAssiutEgypt
  4. 4.Department of Theriogenology, Faculty of Veterinary MedicineMinia UniversityEl MinyaEgypt
  5. 5.Department of StatisticsVirginia polytechnic institute and state universityBlacksburgUSA
  6. 6.Department of Statistics, Mathematics, and InsuranceAssiut UniversityAssiutEgypt

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