Effect of egg white and honey enema on ulcerative colitis in an animal model
- 36 Downloads
Egg and honey contain a variety of bioactive compounds that can influence pro- and anti-inflammatory pathways and have effects on oxidative stress. In this study, the effects of egg white and honey against acetic acid-induced colitis were investigated in rats. Forty adult rats were divided into five groups (n = 7), group I (normal saline), group II (egg white), group III (honey), group IV (Asacol® 100 mg/ml used as a positive control), and group V (combination of honey and egg white 50:50 v/v). Medications (2 cc/day) were administered rectally for a period of 6 days after induction of colitis using 2% acetic acid. Animals were killed on day 7 and colonic biopsies were taken for histopathological scoring. The concentration of malondialdehyde (MDA) and TNF-α was measured on the samples of the colon tissue that were frozen in liquid nitrogen. At 1 week, the histological damage was severe, and the MDA and TNF-α were high throughout the study without any treatment. Honey decreased the TNF-α and MDA tissue levels towards normal levels significantly (P < 0.05). Combination of honey and egg white was not better than honey alone as an anti-inflammatory agent. In the animal model of ulcerative colitis, rectal administration of honey significantly reduced the colonic inflammation. The present study indicates that honey is efficacious in the acetic acid-induced rat colitis model, but these results require further confirmation in human studies and determination of active ingredients.
KeywordsEgg white Honey Ulcerative colitis Surgery
We appreciate Sajad Daneshi for his technical assistant and the laboratory animal center of Shiraz University of Medical Sciences for provision and care of the animals and the RCC center in Shiraz University of Medical Sciences for English editing.
Compliance with ethical standards
Conflict of interest
The author declares that they have no conflict of interest.
This article does contain studies with animals performed by the authors and the study protocol was approved by the Ethics Committee of Shiraz University of Medical Sciences according to the principles of laboratory animal care (no.16273).
- Bilsel Y, Bugra D, Yamaner S, Bulut T, Cevikbas U, Turkoglu U (2002) Could honey have a place in colitis therapy? Effects of honey, prednisolone, and disulfiram on inflammation, nitric oxide, and free radical formation. Dig Surg 19(4):306–311; discussion 311-302. https://doi.org/10.1159/000064580 CrossRefPubMedGoogle Scholar
- Jauregui-Amezaga A, Geerits A, Das Y, Lemmens B, Sagaert X, Bessissow T, Lobaton T, Ferrante M, Van Assche G, Bisschops R, Geboes K, De Hertogh G, Vermeire S (2017) A simplified Geboes Score for ulcerative colitis. J Crohns Colitis 11(3):305–313. https://doi.org/10.1093/ecco-jcc/jjw154 CrossRefPubMedGoogle Scholar
- Kurutas EB, Cetinkaya A, Bulbuloglu E, Kantarceken B (2005) Effects of antioxidant therapy on leukocyte myeloperoxidase and Cu/Zn-superoxide dismutase and plasma malondialdehyde levels in experimental colitis. Mediat Inflamm 2005(6):390–394. https://doi.org/10.1155/mi.2005.390 CrossRefGoogle Scholar
- Lee M, Kovacs-Nolan J, Yang C, Archbold T, Fan MZ, Mine Y (2009) Hen egg lysozyme attenuates inflammation and modulates local gene expression in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Agric Food Chem 57(6):2233–2240. https://doi.org/10.1021/jf803133b CrossRefPubMedGoogle Scholar
- Sninsky CA, Safdi M, Katz S (2008) A new look at a mainstay ulcerative colitis therapy. Gastroenterol Hepatol 4(2 Suppl 4):1–12Google Scholar
- Takhshid MA, Ai J, Alavi SJ, Tavasoli AR (2007) Effects of diets enriched with fish oil, vitamin A and vitamin E on experimental ulcerative colitis in rat’s. Feyz J Kashan Univ Medi Sci 11(3):8–14Google Scholar
- Tanideh N, FAB AJ, Ashraf MJ, Kuhi O, Mehrabani D (2014a) The healing effect of strawberry extract on acetic acid-induced ulcerative colitis in rat. World Appl Sci J 31(3):281–288Google Scholar
- Tanideh N, Afaridi E, Mehrabani D, Azarpira N, Hosseinzadeh M, Amini M, Hosseini S (2014b) The healing effect of Berberis vulgaris in acetic acid-induced ulcerative colitis in rat. Middle-East J Sci Res 21:1288–1294Google Scholar
- Tanideh N, Jamshidzadeh A, Sepehrimanesh M, Hosseinzadeh M, Koohi-Hosseinabadi O, Najibi A, Raam M, Daneshi S, Asadi-Yousefabad SL (2016) Healing acceleration of acetic acid-induced colitis by marigold (Calendula officinalis) in male rats. Saudi J Gastroenterol 22(1):50–56. https://doi.org/10.4103/1319-3767.173759 CrossRefPubMedPubMedCentralGoogle Scholar