Comparative Clinical Pathology

, Volume 28, Issue 5, pp 1483–1493 | Cite as

Biochemical, histopathological, and pharmacological evaluations of cutaneous wound healing properties of Quercus brantii ethanolic extract ointment in male rats

  • Mohammad Mahdi Zangeneh
  • Mehrdad Pooyanmehr
  • Akram ZangenehEmail author
Original Article


The recent experiments have revealed the property of ethnomedicinal plants on the treatment of the cutaneous wound. The purpose of the experiment was to investigate the wound healing and antioxidant potentials of the internal layer of Quercus brantii ethanolic extract ointment. DPPH free radical scavenging test was carried out to examine the antioxidant effect of the internal layer of Q. brantii ethanolic extract, which indicated similar antioxidant activity with butylated hydroxy toluene (BHT) as a positive control. In vivo design, 120 Sprague Dawley male rats were used. After creating the cutaneous wound, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% Q. brantii ethanolic extract ointment (3 g of Q. brantii ethanolic extract + 97 g base ointment). At days 10, 20, and 30 after creating the wound, for histopathological and biochemical analysis of the cutaneous wound healing trend, a section was prepared from all dermal thicknesses. At days 10, 20, and 30, Q. brantii ethanolic extract ointment significantly (p > 0.05) enhanced wound contraction, fibrocyte count, hexuronic acid, and hydroxyproline levels and decreased the wound area, all cell, macrophage, lymphocyte, and neutrophil counts as compared with the basal ointment and control groups. In conclusion, the acquired findings showed the cutaneous wound healing potential of Q. brantii ethanolic extract ointment.


Quercus brantii Ethanolic extract Leaf Cutaneous Wound healing Rats 


Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  2. 2.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran
  3. 3.Department of Microbiology, Pathobiology & Basic Sciences, Faculty of Veterinary MedicineRazi UniversityKermanshahIran

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