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Comparative Clinical Pathology

, Volume 28, Issue 5, pp 1483–1493 | Cite as

Biochemical, histopathological, and pharmacological evaluations of cutaneous wound healing properties of Quercus brantii ethanolic extract ointment in male rats

  • Mohammad Mahdi Zangeneh
  • Mehrdad Pooyanmehr
  • Akram ZangenehEmail author
Original Article

Abstract

The recent experiments have revealed the property of ethnomedicinal plants on the treatment of the cutaneous wound. The purpose of the experiment was to investigate the wound healing and antioxidant potentials of the internal layer of Quercus brantii ethanolic extract ointment. DPPH free radical scavenging test was carried out to examine the antioxidant effect of the internal layer of Q. brantii ethanolic extract, which indicated similar antioxidant activity with butylated hydroxy toluene (BHT) as a positive control. In vivo design, 120 Sprague Dawley male rats were used. After creating the cutaneous wound, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% Q. brantii ethanolic extract ointment (3 g of Q. brantii ethanolic extract + 97 g base ointment). At days 10, 20, and 30 after creating the wound, for histopathological and biochemical analysis of the cutaneous wound healing trend, a section was prepared from all dermal thicknesses. At days 10, 20, and 30, Q. brantii ethanolic extract ointment significantly (p > 0.05) enhanced wound contraction, fibrocyte count, hexuronic acid, and hydroxyproline levels and decreased the wound area, all cell, macrophage, lymphocyte, and neutrophil counts as compared with the basal ointment and control groups. In conclusion, the acquired findings showed the cutaneous wound healing potential of Q. brantii ethanolic extract ointment.

Keywords

Quercus brantii Ethanolic extract Leaf Cutaneous Wound healing Rats 

Notes

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.

References

  1. Bahmani M, Forouzan S, Fazeli-Moghadam E, Rafieian-Kopaei E, Adineh A, Saberianpour S (2015) Oak (Quercus branti): an overview. J Chem Pharm Res 7(1):634–639Google Scholar
  2. Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal Biochem 4(4):330–334CrossRefPubMedGoogle Scholar
  3. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MAC (2016) Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol 54(11):2555–2559CrossRefPubMedGoogle Scholar
  4. Dastmalchi K, Damien DH, Oinonena P, Darwisd Y, Laaksoa I, Hiltunen R (2008) Chemical composition and in vitro antioscidative activity of a lemon balm (Melissa Officinaliss L.) extract. LWT- Food Sci Techno 41:391–400CrossRefGoogle Scholar
  5. Dong Y-L, Fleming RYD, Yan TZ, Herndon DN, Waymack JP (1993) Effect of ibuprofen on the inflammatory response to surgical wounds. J Trauma 35(3):340–343CrossRefPubMedGoogle Scholar
  6. Dwivedi D, Dwivedi M, Malviya S, Singh V (2017) Evaluation ofwound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Complement Med 7(1):79–85CrossRefPubMedGoogle Scholar
  7. Foschi D, Trabucchi E, Musazzi M, Castoldi L, Di Mattia D, Radaelli E, Marazzi M, Franzini P, Berlusconi A (1988) The effects of oxygen free radicals on wound healing. Int J Tissue React 10(6):373–379PubMedGoogle Scholar
  8. Geethalakshmi R, Sakravarthi C, Kritika T, Arul Kirubakaran M, Sarada DVL (2013) Evaluation of antioxidant and wound healing potentials of Sphaeranthus amaranthoides Burm.f. Biomed Res Int 2013:607109CrossRefPubMedPubMedCentralGoogle Scholar
  9. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263CrossRefGoogle Scholar
  10. Goorani S, Zangeneh MM, Koohi MK, Seydi N, Zangeneh A, Souri N, Hosseini MS (2018) Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats. Comp Clin Pathol 28:435–445.  https://doi.org/10.1007/s00580-018-2866-3 CrossRefGoogle Scholar
  11. Goorani S, Shariatifar N, Seydi N, Zangeneh A, Moradi R, Tari B, Nazari F, Zangeneh MM (2019) The aqueous extract of Allium saralicum 1 R.M. Fritsch effectively treat induced anemia. Experimental study on Wistar rats. Orient Pharm Exp Med.  https://doi.org/10.1007/s13596-019-00361-5
  12. Guo S, DiPietro LA (2010) Factors affecting wound healing. J Dent Res 89(3):219–229CrossRefPubMedPubMedCentralGoogle Scholar
  13. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocininduced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463CrossRefGoogle Scholar
  14. Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organometal Chem 32(9):e4458CrossRefGoogle Scholar
  15. Hemmati S, Rashtiani A, Zangeneh MM, Mohammadi P, Zangeneh A, Veisi H (2019) Green synthesis and characterization of silver nanoparticles using Fritillaria flower extract and their antibacterial activity against some human pathogens. Polyhedron 158:8–14CrossRefGoogle Scholar
  16. Hosseinimehr SJ, Mahmoudzadeh A, Ahmadi A, Ashrafi SA, Shafaghati N, Hedayati N (2011) The radioprotective effect of Zataria multiflora against genotoxicity induced by γ irradiation in human blood lymphocytes. Cancer Biother Radiopharm 26:325–329CrossRefPubMedGoogle Scholar
  17. Hosseinkhani A, Falahatzadeh M, Raoofi E, Zarshenas MM (2016) An evidence-based review on wound healing herbal remedies from reports of traditional Persian medicine. J Evid Based Complementary Altern Med 22(2):334–343CrossRefPubMedPubMedCentralGoogle Scholar
  18. Jalalvand AR, Zhaleh M, Goorani S, Zangeneh MM, Seydi N, Zangeneh A, Moradi R (2019) Chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal properties of ethanolic extract of Allium Saralicum R.M. Fritsch leaves rich in linolenic acid, methyl ester. J Photochem Photobiol B 192:103–112CrossRefPubMedGoogle Scholar
  19. Khorasgni A, Karimi AH, Nazem MR (2010) A comparision of healing effects of propolis and silver sulfadia Zine on full thickness skin wounds in rats. Pak Vet J 30(2):72–740Google Scholar
  20. Koh TJ, DiPietro LA (2011) Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med 13:e23CrossRefPubMedPubMedCentralGoogle Scholar
  21. Kumar B, Vijaykumar M, Govindarajan R, Pushpangadan P (2008) Ethnopharmacological approaches to wound healing. Exploring medicinal plants of India. J Ethnopharmacol 12(8):103–110Google Scholar
  22. Lazarus GS, Cooper DM, Knighton DR, Margolis DJ, Pecoraro RE, Rodeheaver G, Robson MC (1994) Definitions and guidelines for assessment of wounds and evaluation of healing. Arch Dermatol 130(4):489–493CrossRefPubMedGoogle Scholar
  23. Malekzadeh F (1986) Antimicrobial activity of Lawsonia inermis L. Appl Microbiol 16(4):663–664Google Scholar
  24. Moradi R, Hajialiani M, Salmani S, Almasi M, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Comp Clin Pathol.  https://doi.org/10.1007/s00580-018-2834-y
  25. Mussel O, Saliva S, Costa A (2003) Mast cell in tissue response to density materials: an adhesive resin, a calcium hydroxide and a glass ionomer cement. J Cell Mol Med 7(2):171–173CrossRefPubMedGoogle Scholar
  26. Nayak BS, Isitor G, Davis EM, Pillai GK (2007) The evidence based wound healing activity of Lawsonia inermis Linn. Phytother Res 21(9):827–831CrossRefPubMedGoogle Scholar
  27. Oryan A, Tabatabaiei Naieni A, Moshiri A, Mohammadalipoor A, Tabandeh MR (2012) Modulation of cutaneous wound healing by Silymarin in rats. J Wound Care 21(9):457–464CrossRefPubMedGoogle Scholar
  28. Phillips GD, Whitehe RA, Kinghton DR (1991) Initiation and pattern of angiogenesis in wound healing in the rats. Am J Anat 192(3):257–262CrossRefPubMedGoogle Scholar
  29. Pithayanukul P, Ruenraroengsak P, Bavovada R, Pakmanee N, Suttisri R, Saen-oon S (2005) Inhibition of Naja kaouthia venom activities by plant polyphenols. J Ethnopharmacol 97(3):527–533CrossRefPubMedGoogle Scholar
  30. Rezvanipour M, Pourzadehhosseini F, Malekpour R, Zarabi A (2007) The effect of mummy on some indices of wound healing in mice. J of Kerman Univ of Med Sci 14(4):77–267Google Scholar
  31. Robards K, Prenzler PD, Tucker G, Swatsitang P, Glover W (1999) Phenolic compounds and their role in oxidative processes in fruits. Food Chem 66(4):401–436CrossRefGoogle Scholar
  32. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Pl 21(1):164–174CrossRefGoogle Scholar
  33. Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543CrossRefGoogle Scholar
  34. Taleshi H, Maasoumi Babarabi M (2013) Leaf morphological variation of Quercus brantii Lindl. Along an altitudinal gradient in Zagros forests of Fars Province, Iran. Euro J Exp Bio 3(5):463–468Google Scholar
  35. Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F (2018a) Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol 27(4):861–867CrossRefGoogle Scholar
  36. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2018b) Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Bio Sci 70(4):655–664CrossRefGoogle Scholar
  37. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018c) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl4-induced hepatic injury in mice. Comp Clin Path 27:1359–1365.  https://doi.org/10.1007/s00580-018-2747-9 CrossRefGoogle Scholar
  38. Zhaleh M, Sohrabi N, Zangeneh MM, Zangeneh A, Moradi R, Zhaleh H (2018) Chemical composition and antibacterial effects of essential oil of Rhus coriaria fruits in the west of Iran (Kermanshah). J Essent Oil Bear Plant 21(2):493–501CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  2. 2.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran
  3. 3.Department of Microbiology, Pathobiology & Basic Sciences, Faculty of Veterinary MedicineRazi UniversityKermanshahIran

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