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Healing and cytotoxicity potentials of ointment containing aqueous extract of Anethum graveolens on cutaneous wounds in male rats

  • Samaneh Goorani
  • Mohammad Kazem Koohi
  • Mohammad Mahdi ZangenehEmail author
  • Niloofar Seydi
  • Akram Zangeneh
  • Pouya Zid-Abdi
  • Milad Moradi
  • Hossein Zhaleh
Original Article

Abstract

Anethum graveolens is a well-known plant in Iranian traditional medicine, and its antioxidative and anti-inflammatory properties make it a logical adjuvant to improve wound healing. The aim of the study was to survey the wound healing property of aqueous extract of A. graveolens. The extract had great cell viability dose dependently, and this method was found to be non-toxic for synthesizing A. graveolens. In this study, 120 Sprague Dawley male rats were used. After creating a cutaneous wound, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% A. graveolens aqueous extract ointment (3 g of A. graveolens aqueous extract + 97 g base ointment). On days 10, 20, and 30 after creating the wound, for biochemical and histopathological analysis of the cutaneous wound healing trend, a section was prepared from all dermal thicknesses. A. graveolens aqueous extract ointment could significantly (p ≤ 0.05) reduce the levels of the wound area, total cell, lymphocyte, and neutrophil and enhance the levels of wound contracture, hydroxyproline, hexosamine, and fibrocyte as compared with the basal ointment and control groups. In conclusion, the present study showed that the application of A. graveolens extract on wounds induces considerable wound contraction and accelerates healing, and it may be suggested for treating different types of wounds in animals and human beings.

Keywords

Anethum graveolens Aqueous extract Wound healing effect 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.

References

  1. Abbasi Oshaghi E, Khodadadi I, Tavilani H, Taghi Goodarzi M (2016) Aqueous extract of Anethum graveolens L. has potential antioxidant and antiglycation affects. Iran J Med Sci 41(4):328–333Google Scholar
  2. Arulmozhi V, Pandian K, Mirunalini S (2013) Ellagic acid encapsulated chitosan nanoparticles for drug delivery system in human oral cancer cell line (KB). Colloids Surf B: Biointerfaces 110:313–320Google Scholar
  3. Azhdari-Zarmehri H, Nazemi S, Ghasemi E, Musavi Z, Tahmasebi Z, Farsad F, Farzam A (2014) Assessment of effect of hydro-alcoholic extract of Scrophularia Striata on burn healing in rat. JBUMS 16(5):42–48Google Scholar
  4. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MAC (2016) Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol 54(11):2555–2559Google Scholar
  5. Dastmalchi K, Damien DH, Oinonena P, Darwisd Y, Laaksoa I, Hiltunen R (2008) Chemical composition and in vitro antioscidative activity of a lemon balm (Melissa officinaliss L.) extract. LWT Food Sci Technol 41:391–400Google Scholar
  6. Dong Y-L, Fleming RYD, Yan TZ, Herndon DN, Waymack JP (1993) Effect of ibuprofen on the inflammatory response to surgical wounds. J Trauma 35(3):340–343Google Scholar
  7. Dwivedi D, Dwivedi M, Malviya S, Singh V (2017) Evaluation ofwound healing, anti-microbial and antioxidant potential of Pongamia pinnata in Wistar rats. J Tradit Complement Med 7(1):79–85Google Scholar
  8. Edziri H, Ammar S, Souad L, Mahjoub MA, Mastouri M, Aouni M, Mighri Z, Verschaeve L (2012) In vitro evaluation of antimicrobial and antioxidant activities of some Tunisian vegetables. S Afr J Bot 78:252–256Google Scholar
  9. Foschi D, Trabucchi E, Musazzi M, Castoldi L, Di Mattia D, Radaelli E, Marazzi M, Franzini P, Berlusconi A (1988) The effects of oxygen free radicals on wound healing. Int J Tissue React 10(6):373–379Google Scholar
  10. Geethalakshmi R, Sakravarthi C, Kritika T, Arul Kirubakaran M, Sarada DVL (2013) Evaluation of antioxidant and wound healing potentials of Sphaeranthus amaranthoides Burm.f. Biomed Res Int 2013:607109Google Scholar
  11. Gharib Naseri MK, Heidari A (2007) Antispasmodic effect of Anethum graveolens fruit extract on rat ileum. Int J Pharmacol 3(3):260–264Google Scholar
  12. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263Google Scholar
  13. Goorani S, Zangeneh MM, Koohi MK, Seydi N, Zangeneh A, Souri N, Hosseini MS (2018) Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats. Comp Clin Pathol 28:435–445.  https://doi.org/10.1007/s00580-018-2866-3 Google Scholar
  14. Goorani S, Shariatifar N, Seydi N, Zangeneh A, Moradi R, Tari B, Nazari F, Zangeneh MM (2019) The aqueous extract of Allium saralicum R.M. Fritsch effectively treat induced anemia: experimental study on Wistar rats. Orient Pharm Exp Med.  https://doi.org/10.1007/s13596-019-00361-5
  15. Guo S, DiPietro LA (2010) Factors affecting wound healing. J Dent Res 89(3):219–229Google Scholar
  16. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463Google Scholar
  17. Hajhashemi V, Abbasi N (2008) Hypolipidemic activity of Anethum graveolens in rats. Phytother Res 22(3):372–375Google Scholar
  18. Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organomet Chem 32(9):e4458Google Scholar
  19. Hemmati S, Rashtiani A, Zangeneh MM, Mohammadi P, Zangeneh A, Veisi H (2019) Green synthesis and characterization of silver nanoparticles using Fritillaria flower extract and their antibacterial activity against some human pathogens. Polyhedron 158:8–14Google Scholar
  20. Hosseinkhani A, Falahatzadeh M, Raoofi E, Zarshenas MM (2016) An evidence-based review on wound healing herbal remedies from reports of traditional Persian medicine. J Evid Based Complementary Altern Med 22(2):334–343Google Scholar
  21. Kaur GJ, Arora DS (2009) Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complement Altern Med 9:30Google Scholar
  22. Khorasgni A, Karimi AH, Nazem MR (2010) A comparison of healing effects of propolis and silver sulfadiazine on full thickness skin wounds in rats. Pak Vet J 30(2):72–740Google Scholar
  23. Koh TJ, DiPietro LA (2011) Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med 13:e23Google Scholar
  24. Kumar B, Vijaykumar M, Govindarajan R, Pushpangadan P (2008) Ethnopharmacological approaches to wound healing. Exploring medicinal plants of India. J Ethnopharmacol 12(8):103–110Google Scholar
  25. Lazarus GS, Cooper DM, Knighton DR, Margolis DJ, Pecoraro RE, Rodeheaver G, Robson MC (1994) Definitions and guidelines for assessment of wounds and evaluation of healing. Arch Dermatol 130(4):489–493Google Scholar
  26. Malekzadeh F (1986) Antimicrobial activity of Lawsonia inermis L. Appl Microbiol 16(4):663–664Google Scholar
  27. Moradi R, Hajialiani M, Salmani S, Almasi M, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Comp Clin Pathol.  https://doi.org/10.1007/s00580-018-2834-y
  28. Mussel O, Saliva S, Costa A (2003) Mast cell in tissue response to density materials: an adhesive resin, a calcium hydroxide and a glass ionomer cement. J Cell Mol Med 7(2):171–173Google Scholar
  29. Nayak BS, Isitor G, Davis EM, Pillai GK (2007) The evidence based wound healing activity of Lawsonia inermis Linn. Phytother Res 21(9):827–831Google Scholar
  30. Oryan A, Tabatabaiei Naieni A, Moshiri A, Mohammadalipoor A, Tabandeh MR (2012) Modulation of cutaneous wound healing by silymarin in rats. J Wound Care 21(9):457–464Google Scholar
  31. Panda S (2008) The effect of Anethum graveolens L. (dill) on corticosteroid induced diabetes mellitus: involvement of thyroid hormones. Phytother Res 22(12):1695–1697Google Scholar
  32. Phillips GD, Whitehe RA, Kinghton DR (1991) Initiation and pattern of angiogenesis in wound healing in the rats. Am J Anat 192(3):257–262Google Scholar
  33. Rezvanipour M, Pourzadehhosseini F, Malekpour R, Zarabi A (2007) The effect of mummy on some indices of wound healing in mice. J of Kerman Univ of Med Sci 14(4):77–267Google Scholar
  34. Robards K, Prenzler PD, Tucker G, Swatsitang P, Glover W (1999) Phenolic compounds and their role in oxidative processes in fruits. Food Chem 66(4):401–436Google Scholar
  35. Sahraei M, Zangeneh MM, Khanahmadi M, Moradi R, Kazemi N, Zhaleh H, Amini K (2019) The effect of Descurainia sophia oil on methamphetamine-induced cell cytotoxicity and cell death in PC12. Comp Clin Pathol.  https://doi.org/10.1007/s00580-019-02968-1
  36. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Pl 21(1):164–174Google Scholar
  37. Shakibaie D, Pasharavesh L, Khoshboo S, Kaboodi B (2007) The effect of the “Falcaria vulgaris” on deep skin wound remodeling time and skin tension power in rats. J Kermanshah Univ Med Sci 10(3):187–194Google Scholar
  38. Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543Google Scholar
  39. Souba WW, Wilmore D (1999) Diet and nutrition in case of the patient with surgery. 9th Ed. Williams and Wilkins Press, Baltimore, pp 1589–1618Google Scholar
  40. Srivastava AK, Kushawaha DK, watal G (2017) Antioxidant and preliminary phytochemical studies of Anethum graveolens. Int J Pharm Bio Sci 8(2):364–373Google Scholar
  41. Yung-Shin S, Jau-Tien L, Yuan-Tsung C, Chia-Jung C, Deng-Jye Y (2009) Evaluation of antioxidant ability of ethanolic extract from dill (Anethum graveolens L.) flower. Food Chem 115(2):515–521Google Scholar
  42. Zangeneh MM, Goodarzi N, Zangeneh A, Tahvilian R, Najafi F (2018a) Amelioration of renal structural changes in STZ-induced diabetic mice with ethanolic extract of Allium saralicum R.M. Fritsch. Comp Clin Pathol 27(4):861–867Google Scholar
  43. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2018b) Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Bio Sci 70(4):655–664Google Scholar
  44. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018c) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl4-induced hepatic injury in mice. Comp Clin Pathol 27:1359–1365.  https://doi.org/10.1007/s00580-018-2747-9 Google Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Toxicology, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  2. 2.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  3. 3.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran
  4. 4.Substance Abuse Prevention Research CenterKermanshah University of Medical SciencesKermanshahIran

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