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Comparative Clinical Pathology

, Volume 28, Issue 4, pp 1181–1190 | Cite as

Pharmacological, biochemical, and histopathological evaluations of cutaneous wound healing property of Mentha piperita aqueous extract ointment

  • Mohammad Mahdi Zangeneh
  • Akram Zangeneh
  • Rohallah Moradi
  • Niloofar SeydiEmail author
Original Article

Abstract

In our study, we evaluated cutaneous wound healing potential of Mentha piperita aqueous extract ointment in rats. In vivo design, after creating the cutaneous wound on the back of the rats, the animals were randomly divided into four groups: untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, and treatment with 3% M. piperita aqueous extract ointment. The groups were treated for 30 days. For biochemical and histopathological analysis of the cutaneous wound healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses on days 10, 20, and 30. The data were analyzed by SPSS-21 software. The use of M. piperita aqueous extract ointment in the treatment groups led to remarkable reduction (p < 0.05) in the levels of wound area, total cells, neutrophil, lymphocyte, and macrophage and remarkable increase (p < 0.05) in the levels of wound contracture, hydroxyl proline, hexosamine, hexuronic acid, fibrocyte, and the rate of fibrocyte to fibroblast compared with the control and basal ointment groups. According to the findings, M. piperita aqueous extract ointment can cure the cutaneous wound.

Keywords

Mentha piperita Aqueous extract Cutaneous wound healing 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

All institutional and national guidelines for the care and use of laboratory animals were followed.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  2. 2.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran

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