Advertisement

Comparative Clinical Pathology

, Volume 28, Issue 5, pp 1281–1286 | Cite as

Gallic acid reduces inflammatory cytokines and markers of oxidative damage in a rat model of estradiol-induced polycystic ovary

  • Bibi Fatemeh Mazloom
  • Mohammad Amin EdalatmaneshEmail author
  • Seyed Ebrahim Hosseini
Original Article
  • 46 Downloads

Abstract

Hormonal disorders, oxidative stress, and inflammation in ovarian tissue cause ovulation failure in women with polycystic ovary syndrome. Considering the antioxidant properties of gallic acid (GA), the aim of this study was to investigate the effect of GA on pro-inflammatory cytokines, antioxidant enzyme activity, and DNA oxidative damage of ovarian tissue in a rat model of estradiol-induced polycystic ovary (PCO). In this experimental study, 32 female Wistar rats were divided into four groups. These included control (saline solution, orally), PCO+saline (estradiol valerate + saline solution, orally), PCO+GA50, and PCO+GA100 (estradiol valerate +50 and 100 mg/kg gallic acid, orally), respectively. The PCO model was induced by a single intramuscular injection of estradiol valerate (EV, 4 mg/kg). Twenty-four days after PCO modeling, tissue concentration of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) and also the activity level of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA), and 8-oxo-2′-deoxyguanosine (8-OHdG) in ovarian tissue were measured through ELISA technique. Compared with the PCO+saline, in the GA-treated group, the concentration of TNF-α, IL-1β, and IL-6 in a dose-dependent manner significantly decreased (p > 0.05). In addition, the activity level of SOD, CAT, and GPX enzymes significantly increased (p < 0.05), while the amount of MDA and 8-OHdG significantly decreased (p > 0.05) in a dose-dependent manner. GA is an anti-inflammatory and an antioxidant polyphenol which reduces the concentration of inflammatory cytokines, oxidative stress, lipid peroxidation, and DNA oxidative damage of ovarian tissue in a rat’s model of PCO.

Keywords

Polycystic ovary syndrome Gallic acid Inflammatory cytokine Oxidative stress Rat 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed.

References

  1. Alchami A, O’Donovan O, Davies M (2015) PCOS: diagnosis and management of related infertility. Obstet Gynaecol Reprod Med 25(10):279–282CrossRefGoogle Scholar
  2. Boots CE, Jungheim ES (2015) Inflammation and human ovarian follicular dynamics. Semin Reprod Med 33(4):270–275CrossRefPubMedPubMedCentralGoogle Scholar
  3. Ciaraldi TP, Aroda V, Mudaliar SR, Henry RR (2013) Inflammatory cytokines and chemokines, skeletal muscle and polycystic ovary syndrome: effects of pioglitazone and metformin treatment. Metabolism 62(11):1587–1596CrossRefPubMedGoogle Scholar
  4. Cohen G, Riahi Y, Sunda V, Deplano S, Chatgilialoglu C, Ferreri C, Kaiser N, Sasson S (2013) Signaling properties of 4-hydroxyalkenals formed by lipid peroxidation in diabetes. Free RadicBiol Med 65:978–987CrossRefGoogle Scholar
  5. Dikmen A, Ergenoglu AM, Yeniel AO, Dilsiz OY, Ercan G, Yilmaz H (2012) Evaluation of glycemic and oxidative/antioxidative status in the estradiol valerate-induced PCOS model of rats. Eur J Obstet Gynecol Reprod Biol 160(1):55–59CrossRefPubMedGoogle Scholar
  6. Farbood Y, Sarkaki A, Hashemi S, Mansouri MT, Dianat M (2013) The effects of gallic acid on pain and memory following transient global ischemia/reperfusion in wistar rats. Avicenna J Phytomed 3(4):329–340PubMedPubMedCentralGoogle Scholar
  7. Furat Rencber S, Kurnaz Ozbek S, Eraldemır C, Sezer Z, Kum T, Ceylan S, Guzel E (2018) Effect of resveratrol and metformin on ovarian reserve and ultrastructure in PCOS: an experimental study. J Ovarian Res 11(1):55CrossRefPubMedPubMedCentralGoogle Scholar
  8. Ghowsi M, Khazali H, Sisakhtnezhad S (2018) Evaluation of TNF-α and IL-6 mRNAs expressions in visceral and subcutaneous adipose tissues of polycystic ovarian rats and effects of resveratrol. Iran J Basic Med Sci 21(2):165–174PubMedPubMedCentralGoogle Scholar
  9. Goodarzi MO, Carmina E, Azziz R (2015) DHEA, DHEAS and PCOS. J Steroid Biochem Mol Biol 145:213–225CrossRefPubMedGoogle Scholar
  10. Hsu W, Chang S, Lin L, Li C, Richardson C, Lin C et al (2015) Limonium sinense and gallic acid suppress hepatitis C virus infection by blocking early viral entry. Antivir Res 118:139–147CrossRefPubMedGoogle Scholar
  11. Jelodar G, Masoomi S, Rahmanifar F (2018) Hydroalcoholic extract of flaxseed improves polycystic ovary syndrome in a rat model. Iran J Basic Med Sci 21(6):645–650PubMedPubMedCentralGoogle Scholar
  12. Joham AE, Palomba S, Hart R (2016) Polycystic ovary syndrome, obesity, and pregnancy. Semin Reprod Med 34(2):93–101CrossRefPubMedGoogle Scholar
  13. Kroes BH, van den Berg AJ, Quarles van Ufford HC, van Dijk H, Labadie RP (1992) Anti-inflammatory activity of gallic acid. Planta Med 58:499–504CrossRefPubMedGoogle Scholar
  14. Li B, Weng Q, Liu Z, Shen M, Zhang J, Wu W, Liu H (2017) Selection of antioxidants against ovarian oxidative stress in mouse model. J Biochem Mol Toxicol 31(12):e21997CrossRefGoogle Scholar
  15. Lu J, Wang Z, Cao J, Chen Y, Dong Y (2018) A novel and compact review on the role of oxidative stress in female reproduction. Reprod Biol Endocrinol 16(1):80CrossRefPubMedPubMedCentralGoogle Scholar
  16. Matsuzaki T, Tungalagsuvd A, Iwasa T, Munkhzaya M, Yanagihara R, Tokui T, Yano K, Mayila Y, Kato T, Kuwahara A, Matsui S, Irahara M (2017) Kisspeptin mRNA expression is increased in the posterior hypothalamus in the rat model of polycystic ovary syndrome. Endocr J 64(1):7–14CrossRefPubMedGoogle Scholar
  17. Naderpoor N, Shorakae S, de Courten B, Misso ML, Moran LJ, Teede HJ (2016) Metformin and lifestyle modification in polycystic ovary syndrome: systematic review and meta-analysis. Hum Reprod Update 21(5):560–557CrossRefGoogle Scholar
  18. Palomo J, Dietrich D, Martin P, Palmer G, Gabay C (2015) The interleukin (IL)-1 cytokine family - balance between agonists and antagonists in inflammatory diseases. Cytokine 76(1):25–37CrossRefPubMedGoogle Scholar
  19. Pandurangan AK, Mohebali N, Esa NM, Looi CY, Ismail S, Saadatdoust Z (2015) Gallic acid suppresses inflammation in dextran sodium sulfate-induced colitis in mice: possible mechanisms. Int Immunopharmacol 28(2):1034–1043CrossRefPubMedGoogle Scholar
  20. Priscilla DH, Prince PS (2009) Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact 179(2–3):118–124CrossRefPubMedGoogle Scholar
  21. Roidoung S, Dolan KD, Siddiq M (2016) Gallic acid as a protective antioxidant against anthocyanin degradation and color loss in vitamin-C fortified cranberry juice. Food Chem 210:422–427CrossRefPubMedGoogle Scholar
  22. Santbrink EJP, Fauser BCJM (2006) Ovulation induction in normo-gonadotropic anovulation (PCOS). Best Pract Res Clin Endocrinol Metab 20(2):261–270CrossRefPubMedGoogle Scholar
  23. Setyaningsih Y, Husodo AH, Astuti I (2015) Detection of urinary 8-hydroxydeoxyguanosine (8-OHdG) levels as a biomarker of oxidative DNA damage among home industry workers exposed to chromium. Procedia Environ Sci 23:290–296CrossRefGoogle Scholar
  24. Shi Y, Kong X, Yin H, Zhang W, Wang W (2018) Effect of hawthorn leaf flavonoids in dehydroepiandrosterone-induced polycystic ovary syndrome in rats. Pathobiology 7:1–9Google Scholar
  25. Walters KA, Allan CM, Handelsman DJ (2012) Rodent models for human polycystic ovary syndrome. Biol Reprod 86(5):1–12CrossRefGoogle Scholar
  26. Wang Y, Zhu W (2012) Evaluation of adiponectin, resistin, IL-6, TNF-α in obese and non-obese women with polycystic ovary syndrome. Reprod Contracept 23(4):237–244CrossRefGoogle Scholar
  27. Yao X, Huang J, Zhong H, Shen N, Faggioni R, Fung M, Yao Y (2014) Targeting interleukin-6 in inflammatory autoimmune diseases and cancers. Pharmacol Ther 141(2):125–139CrossRefPubMedGoogle Scholar
  28. Yao Y, Wu M, Huang Y, Li C, Pan X, Zhu W, Huang Y (2017) Appropriately raising fermentation temperature beneficial to the increase of antioxidant activity and gallic acid content in Eurotium cristatum-fermented loose tea. LWT Food Sci Technol 82:248–254CrossRefGoogle Scholar
  29. Yigitturk G, Acara AC, Erbas O, Oltulu F, Yavasoglu NUK, Uysal A, Yavasoglu A (2017) The antioxidant role of agomelatine and gallic acid on oxidative stress in STZ induced type I diabetic rat testes. Biomed Pharmacother 87:240–246CrossRefPubMedGoogle Scholar
  30. Yoshino M, Haneda M, Naruse M, Htay HH, Iwata S, Tsubouchi R, Murakami K (2002) Prooxidant action of gallic acid compounds: copper-dependent strand breaks and the formation of 8-hydroxy-2′-deoxyguanosine in DNA. Toxicol in Vitro 16(6):705–709CrossRefPubMedGoogle Scholar
  31. Zuo T, Zhu M, Xu W (2016) Roles of oxidative stress in polycystic ovary syndrome and cancers. Oxidative Med Cell Longev 2016:8589318CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biology, College of Sciences, Shiraz BranchIslamic Azad UniversityShirazIran

Personalised recommendations