Comparative Clinical Pathology

, Volume 28, Issue 5, pp 1281–1286 | Cite as

Gallic acid reduces inflammatory cytokines and markers of oxidative damage in a rat model of estradiol-induced polycystic ovary

  • Bibi Fatemeh Mazloom
  • Mohammad Amin EdalatmaneshEmail author
  • Seyed Ebrahim Hosseini
Original Article


Hormonal disorders, oxidative stress, and inflammation in ovarian tissue cause ovulation failure in women with polycystic ovary syndrome. Considering the antioxidant properties of gallic acid (GA), the aim of this study was to investigate the effect of GA on pro-inflammatory cytokines, antioxidant enzyme activity, and DNA oxidative damage of ovarian tissue in a rat model of estradiol-induced polycystic ovary (PCO). In this experimental study, 32 female Wistar rats were divided into four groups. These included control (saline solution, orally), PCO+saline (estradiol valerate + saline solution, orally), PCO+GA50, and PCO+GA100 (estradiol valerate +50 and 100 mg/kg gallic acid, orally), respectively. The PCO model was induced by a single intramuscular injection of estradiol valerate (EV, 4 mg/kg). Twenty-four days after PCO modeling, tissue concentration of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) and also the activity level of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA), and 8-oxo-2′-deoxyguanosine (8-OHdG) in ovarian tissue were measured through ELISA technique. Compared with the PCO+saline, in the GA-treated group, the concentration of TNF-α, IL-1β, and IL-6 in a dose-dependent manner significantly decreased (p > 0.05). In addition, the activity level of SOD, CAT, and GPX enzymes significantly increased (p < 0.05), while the amount of MDA and 8-OHdG significantly decreased (p > 0.05) in a dose-dependent manner. GA is an anti-inflammatory and an antioxidant polyphenol which reduces the concentration of inflammatory cytokines, oxidative stress, lipid peroxidation, and DNA oxidative damage of ovarian tissue in a rat’s model of PCO.


Polycystic ovary syndrome Gallic acid Inflammatory cytokine Oxidative stress Rat 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biology, College of Sciences, Shiraz BranchIslamic Azad UniversityShirazIran

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