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Effect of zinc on erythrocyte osmotic fragility and hemogram following chronic exposure to glyphosate-based herbicide in Wistar rats

  • Emmanuel Vandi TizheEmail author
  • Najume Dogon-Giginya Ibrahim
  • Mohammed Yakasai Fatihu
  • Suleiman Folorunsho Ambali
  • Ikechukwu Onyebuchi Igbokwe
  • Delia Ussa Tizhe
Original Article
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Abstract

The study was conducted to assess the effect of zinc on erythrocyte osmotic fragility and hemogram of rats following chronic oral exposure to glyphosate-based herbicide (BUSHFIRE®) at daily doses of 14.4–750 mg/kg of glyphosate. The erythrocyte osmotic fragility, estimated as percentage hemolysis in hypotonic saline, was significantly (p < 0.05) increased in glyphosate-exposed groups at 0.5–0.9% NaCl concentrations, and the fragility was ameliorated in the groups with zinc pretreatments only at 0.5% NaCl. The toxicity did not have any significant effect on the packed cell volume and other erythrocyte parameters. There was significant (p < 0.05) decrease in total white blood cell and lymphocyte counts in glyphosate-exposed groups, which was absent in zinc-pretreated groups. In conclusion, increased erythrocyte osmotic fragility induced by glyphosate could be prevented following zinc pretreatment, but the altered leukogram caused by the toxic action was not fully ameliorated in the pretreated group.

Keywords

Glyphosate Zinc Erythrocyte osmotic fragility Leukogram Toxicity 

Notes

Acknowledgements

We hereby acknowledge the contribution of Dr. Uchendu Chidiebere, Department of Veterinary Pharmacology, Physiology and Biochemistry, University of Jos who helped us with the data analysis.

Compliance with ethical standards

This work was carried out after approval was obtained from the ethical committee on the care and use of animals in research of Ahmadu Bello University, Zaria, Nigeria.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Ambali, S. F., Abubakar, A. T., Shittu, M., Yaqub, S.L. Anafi, S.B. and Abdullahi,A. (2010). Chlorpyrifos-induced alteration of haematological parameters in Wistar rats : ameliorative effects of zinc. Res J Environ Toxicol, 4: 55–66Google Scholar
  2. Beuret CJ, Zirulnik F, Giménez MS (2004) Effects of the herbicide glyphosate on liver lipoperoxidation in pregnant rats and their fetuses. Reprod Toxicol 19(4):501–504CrossRefGoogle Scholar
  3. Daruich J, Zirulnik F, Gimenez MS (2001) Effect of the herbicide glyphosate on enzymatic activity in pregnant rats and their fetuses. Environ Res 85:226–231CrossRefGoogle Scholar
  4. Goel A, Danni V, Dhawan DK (2006) Role of zinc in mitigating toxic effects of chlorpyrifos on haematological alterations and electron microscopic observations in rat blood. Biometals 19(15):483–492CrossRefGoogle Scholar
  5. Organization for Economic Cooperation and Development (2009). Chronic toxicity studies- duration of study. OECD Guideline for Testing Chemicals, 452Google Scholar
  6. Olorunsogo O, Bababunmi E, Bassir O (1979) Effect of glyphosate on rat liver mitochondria in vivo. Bull Environ Toxicol 2:257–264Google Scholar
  7. Oyewale JO (1993) Effect of the storage of blood on the osmotic fragility of mammalian erythrocytes. J Vet Med 40:258–264CrossRefGoogle Scholar
  8. Parrat MO, Richard MJ, Beani JC, Favier A (1997) Involvement of zinc in intracellular oxidant/antioxidant balance. Biol Trace Elem Res 60:187–204CrossRefGoogle Scholar
  9. Prasad AS, Kucuk O (2002) Zinc in cancer prevention. Cancer Metastasis Rev 21:291–295CrossRefGoogle Scholar
  10. Sahin A, Kucuk O (2003) Zinc supplementation alleviates heat stress in laying Japanese quail. J Nutr 133:2808–2811CrossRefGoogle Scholar
  11. Schronbrunn E, Eschenburg S, Schuttleworth WA, Schloss JV, Amrhein N, Evans JNS, Kabsch W (2001) Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proc Natl Acad Sci USA 98:1376–1380CrossRefGoogle Scholar
  12. Teruel M, Garcia V, Catalano R (2009) Effects of albendazole sulphoxide on embryonic, foetal and placental parameters in Wistar rats. Int J Morphol 27(4):1147–1153CrossRefGoogle Scholar
  13. Tizhe EV, Ibrahim NDG, Fatihu MY, Igbokwe IO, George BDJ, Ambali SF, Shallangwa JM (2013) Haematological changes induced by subchronic glyphosate exposure: ameliorative effect of zinc in Wistar rats. Sokoto J Vet Sci 11(2):28–35Google Scholar
  14. Tizhe EV, Ibrahim NDG, Fatihu MY, Igbokwe IO, George BDJ, Ambali SF, Shallangwa JM (2014) Influence of zinc supplementation on histopathological changes in the stomach, liver, kidney, brain, pancreas and spleen during subchronic exposure of Wistar wats to glyphosate. Comp Clin Pathol 23:1535–1543Google Scholar
  15. Williams GM, Kroes R, Munro IC (2000) Safety evaluation and risk assessment of the herbicide Roundup® and its active ingredient, glyphosate, for human. Regul Toxicol Pharmacol 31:117–165CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2019

Authors and Affiliations

  • Emmanuel Vandi Tizhe
    • 1
    Email author
  • Najume Dogon-Giginya Ibrahim
    • 2
  • Mohammed Yakasai Fatihu
    • 2
  • Suleiman Folorunsho Ambali
    • 3
  • Ikechukwu Onyebuchi Igbokwe
    • 4
  • Delia Ussa Tizhe
    • 5
  1. 1.Department of Veterinary Microbiology and Pathology, Faculty of Veterinary MedicineUniversity of JosJosNigeria
  2. 2.Department of Veterinary Pathology, Faculty of Veterinary MedicineAhmadu Bello UniversityZariaNigeria
  3. 3.Department of Veterinary Physiology and Pharmacology, Faculty of Veterinary MedicineUniversity of IlorinIlorinNigeria
  4. 4.Department of Veterinary Pathology, Faculty of Veterinary MedicineUniversity of MaiduguriMaiduguriNigeria
  5. 5.Department of Veterinary Medicine, Faculty of Veterinary MedicineAhmadu Bello UniversityZariaNigeria

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