Pathology, isolation and characterisation of virulent and diverse Mannheimia haemolytica and Pasteurella multocida associated with fatal pneumonia in sheep, Rajasthan, India
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Pneumonia, a major sheep disease, causes high mortality among affected animals predominantly the lambs. It is caused by a number of microbes including Mannheimia haemolytica/Pasteurella multocida. It was aimed to explore the pathology in the lungs due to pneumonia, and to isolate and characterise the virulent M. haemolytica/P. multocida strains. The lung tissues and heart blood specimens were collected by necropsy of sheep (n = 96). These samples were used for isolation and characterisation of M. haemolytica/P. multocida. Moreover, the fresh and square-shaped lung pieces were also taken to perform the histopathological analysis. Grossly, the petechial and ecchymotic haemorrhages, congestion and consolidation in lung lobes were observed at necropsy. Pneumonia was diagnosed as acute fibrinous-bronchopneumonia (36), suppurative chronic bronchopneumonia (10), acute interstitial pneumonia (32) and acute pulmonary congestion (18) based on histological lesions. Bacterial isolation recovered M. haemolytica alone or along with P. multocida and/or Bibersteinia trehalosi identified by phenotypic characters and sequencing of 16S rRNA. Haemolytic colonies were documented to carry the PHSSA, plpE and Rpt2 genes. P. multocida identification at species level was carried out by KMT1 gene detection. These isolates were noted to carry the capsule types A (hyaD-hyaC gene) and D (dcbF gene) and outer membrane protein genes ompH and omp87. Sequencing revealed nucleotide variability in Rpt2, ompH and omp87 genes.We highlighted the occurrence of fatal ovine pneumonic pasteurellosis/mannheimiosis caused and/or complicated by virulent M. haemolytica/P. multocida strains.
KeywordsBacterial infections Mannheimia haemolytica Pasteurella multocida Pathology Pneumonia Virulence
The authors are thankful to Dr. Dhirendra Singh, then Head of the Animal Health Division and the Director of ICAR-Central Sheep and Wool Research Institute, Avikanagar, Tonk, Rajasthan, India, for providing the necessary facilities to conduct the research work.
Compliance with ethical standards
This research received no specific grant from any funding agency, commercial or not-for profit sectors.
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Black P, Nunn M (2009) Impact of climate change and environmental change on emerging and re-emerging animal diseases and animal production. Proceedings of technical items presented in 77th general session of the OIE world assembly of delegates, 24–29 May, Paris, France, pp 15Google Scholar
- Brogden KA, Lehmkuhl HD, Cutlip RC (1998) Pasteurella haemolytica complicated respiratory infections in sheep and goats. Vet Res 29:233–254Google Scholar
- CLSI (2008) Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals- Document M31-A3, Wayne, Pennsylvania, USAGoogle Scholar
- CLSI (2010) Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria-Document M45-A2, Wayne, Pennsylvania, USAGoogle Scholar
- Dassanayake RP, Shanthalingam S, Herndon CN, Subramaniam R, Lawrence PK, Bavananthasivam J, Cassirer EF, Haldorson GJ, Foreyt WJ, Rurangirwa FR, Knowles DP, Besser TE, Srikumaran S (2010) Mycoplasma ovipneumoniae can predispose bighorn sheep to fatal Mannheimia haemolytica pneumonia. Vet Microbiol 145:354–359CrossRefGoogle Scholar
- Dassanayake RP, Shanthalingam S, Herndon CN, Subramaniam R, Herndon CN, Bavananthasivam J, Haldorson GJ, Foreyt WJ, Evermann JF, Herrmann-Hoesing LM, Knowles DP, Srikumaran S (2013) Role of Bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia. Vet Microbiol 162:166–172CrossRefGoogle Scholar
- Deressa A, Asfaw Y, Lubke B, Kyule MW, Tefera G, Zessin KH (2010) Molecular detection of Pasteurella multocida and Mannheimia haemolytica in sheep respiratory infections in Ethiopia. Int J Appl Res Vet Med 8:101–108Google Scholar
- Dixit SK, Singh S, Tripathi BN, Singh F, Sonawane GG, Kumar J (2010) Therapeutic management of pneumonia in sheep. Vet Pract 11:111–113Google Scholar
- Gonzalez CT, Maheswaran SK, Murtaugh MP (1995) Pasteurella haemolytica serotype 2 contains the gene for a noncapsular serotype 1-specific antigen. Infect Immun 63:1340–1348Google Scholar
- Harmon BG, Glisson JR, Latimer KS, Steffens WL, Nunnally JC (1991) Resistance of Pasteurella multocida A: 3, 4 to phagocytosis by turkey macrophages and heterophils. Am J Vet Res 52:1507–1511Google Scholar
- Hawari AD, Hassawi DS, Sweiss M (2008) Isolation and identification of Mannheimia haemolytica and Pasteurella multocida in sheep and goats using biochemical tests and random amplified polymorphic DNA (RAPD) analysis. J Biol Sci (7):1251–1254Google Scholar
- Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackenbrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
- Luna LG (1968) Manual of histologic staining methods of the Armed Forces. Institute of Pathology, New YorkGoogle Scholar
- Miller MW (2001) Pasteurellosis. In: Williams ES, Barker IK (eds) Infectious diseases of wild mammals. Iowa State University Press, Ames, p 558Google Scholar
- Mohamed RA, Abdelsalam EB (2008) A review on pneumonic Pasteurellosis (respiratory Mannheimiosis) with emphasis on pathogenesis, virulence mechanism and predisposing factors. Bulg J Vet Med 11:139–160Google Scholar
- Singh R, Tewari K, Packiriswamy N, Marla S, Rao VDP (2011) Molecular characterization and computational analysis of the major outer membrane protein (OmpH) gene of Pasteurella multocida P52. Vet Arh 81:211–222Google Scholar
- Townsend KM, Frost AJ, Lee CW, Papadimitriou JM, Dawkins HJS (1998) Development of PCR assays for species and type specific identification of Pasteurella multocida isolates. J Clin Microbiol 36:1096–1100Google Scholar
- Valadan M, Jabbari AR, Niroumand M, Tahamtan Y, Bani Hashemi SR (2014) Isolation and identification of Pasteurella multocida from sheep & goat in Iran. Arch Razi Inst 69:47–55Google Scholar