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Phylogenetic evaluation of Macrorhabdus ornithogaster isolated from a case of canary (Serinus canaria)

  • Bahman Abdi-Hachesoo
  • Hassan Sharifiyazdi
  • Mohamad Reza Haddad-Marandi
  • Mohsen Kalantari
Case Report
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Abstract

Two dead birds from a flock of domestic canaries (Serinus canaria) were referred with a history of anorexia, weight loss, abdominal distension, and occasional mortality. A slightly enlarged liver and proventriculus were seen on the examination of the abdominal cavity. A large number of yeast resembling Macrorhabdus ornithogaster were observed on gram staining of the proventriculus mucosa. Pseudomonas aeruginosa was isolated on the microbial culture of the liver. The complete genomic sequence of the internal transcribed spacer 1 (ITS1) was determined in Macrorhabdus ornithogaster using panfungal primers. The alignment of the sequence obtained from this study with 6 GenBank deposited ITS1sequences of Macrorhabdus ornithogaster showed some repeated nucleotide substitutions. The complete ITS1 sequence of our study (MF993119) displayed a whole genetic similarity to ITS1 sequences of Macrorhabdus ornithogaster isolated from canaries and finches and clustered into a different group from ITS1 sequences of Macrorhabdus ornithogaster isolated from pssitacines. The ITS1 region is proposed as taxonomically informative for Macrorhabdus ornithogaster isolates from different hosts.

Keywords

Macrorhabdus ornithogaster Pseudomonas aeruginosa Serinus canaria Phylogeny 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

References

  1. Arabkhazaeli F, Madani SA, Ghavami S (2016) Outbreak of an unusual tracheal mite, Ptilonyssus morofskyi (Acarina: Rhinonyssidae), in canaries (Serinus canaria) with concurrent infection with Staphylococcus aureus and Macrorhabdus ornithogaster. J Avian Med Surg 30(3):269–273CrossRefPubMedGoogle Scholar
  2. Behnke EL, Fletcher OJ (2011) Macrorhabdus ornithogaster (Megabacterium) infection in adult hobby chickens in North America. Avian Dis 55(2):331–334CrossRefPubMedGoogle Scholar
  3. Dorrestein GM (2009) Bacterial and parasitic diseases of passerines. Vet Clin North Am Exot Anim Pract 12(3):433–451CrossRefPubMedGoogle Scholar
  4. Hannafusa Y, Bradley A, Tomaszewski EE, Libal MC, Phalen DN (2007) Growth and metabolic characterization of Macrorhabdus ornithogaster. J Vet Diagn Investig 19(3):256–265CrossRefGoogle Scholar
  5. Lumbsch HT, Schmitt I, Döring H, Wedin M (2001) ITS sequence data suggest variability of ascus types and support ontogenetic characters as phylogenetic discriminators in the Agyriales (Ascomycota). Mycol Res 105(3):265–274CrossRefGoogle Scholar
  6. Madani SA, Haddad-Marandi MR, Arabkhazaeli F (2015) Concurrent atypical diffused tuberculosis and macrorhabdosis in a canary (Serinus canaria). Vet Res Forum 6(1):89–93PubMedPubMedCentralGoogle Scholar
  7. Martins NR, Horta AC, Siqueira AM, Lopes SQ, Resende JS, Jorge MA, Assis RA, Martins NE, Fernandes AA, Barrios PR, Costa TJ (2006) Macrorhabdus ornithogaster in ostrich, rhea, canary, zebra finch, free range chicken, turkey, guinea-fowl, columbina pigeon, toucan, chuckar partridge and experimental infection in chicken, japanese quail and mice. Arq Bras Med Vet 58(3):291–298CrossRefGoogle Scholar
  8. Nemeth NM, Gonzalez-Astudillo V, Oesterle PT, Howerth EW (2016) A 5-year retrospective review of avian diseases diagnosed at the Department of Pathology, University of Georgia. J Comp Pathol 155(2–3):105–120CrossRefPubMedGoogle Scholar
  9. Phalen DN (2014) Update on the diagnosis and management of Macrorhabdus ornithogaster (formerly megabacteria) in avian patients. Vet Clin North Am Exot Anim Pract 17(2):203–210CrossRefPubMedGoogle Scholar
  10. Phalen DN (2016) Macrorhabdus Ornithogaster. In: Samour J (ed) Avian Medicine. Elsevier, Amsterdam, pp 473–477Google Scholar
  11. Piasecki T, Prochowska S, Celmer Ż, Sochacka A, Bednarski M (2012) Occurrence of Macrorhabdus ornithogaster in exotic and wild birds in Poland. Med Weter 68(4):245–249Google Scholar
  12. Quinn PJ, Carter ME, Markey B, Carter GR (1994) Veterinary clinical microbiology. Wolfe Publication, London, UK, pp 237–242Google Scholar
  13. Samour JH (2000) Pseudomonas aeruginosa stomatitis as a sequel to trichomoniasis in captive saker falcons (Falco cherrug). J Avian Med Surg 14(2):113–117CrossRefGoogle Scholar
  14. Sullivan PJ, Ramsay EC, Greenacre CB, Cushing AC, Zhu X, Jones MP (2017) Comparison of two methods for determining prevalence of Macrorhabdus ornithogaster in a flock of captive budgerigars (Melopsittacus undulatus). J Avian Med Surg 31(2):128–131CrossRefPubMedGoogle Scholar
  15. Tomaszewski EK, Logan KS, Snowden KF, Kurtzman CP, Phalen DN (2003) Phylogenetic analysis identifies the ‘megabacterium’of birds as a novel anamorphic ascomycetous yeast, Macrorhabdus ornithogaster gen. nov., sp. nov. Int J Syst Evol Microbiol 53(4):1201–1205CrossRefPubMedGoogle Scholar
  16. Visentin I, Tamietti G, Valentino D, Portis E, Karlovsky P, Moretti A, Cardinale F (2009) The ITS region as a taxonomic discriminator between Fusarium verticillioides and Fusarium proliferatum. Mycol Res 113(10):1137–1145CrossRefPubMedGoogle Scholar
  17. White TJ, Bruns T, Lee SJ, Taylor JL (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols 18(1):315–322Google Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Sciences, School of Veterinary MedicineShiraz UniversityShirazIran

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