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Comparative Clinical Pathology

, Volume 28, Issue 2, pp 447–453 | Cite as

An epidemiological survey of bovine viral diarrhea infection in calves in Egypt with identification of high prevalence of persistent infected animals

  • Samar M. Atwa
  • Younis E. Emad
  • Mohamed Abdo RizkEmail author
  • Mohamed El-Beskawy
  • Sayed M. Zeidan
Original Article
  • 42 Downloads

Abstract

In the present study, we identified the persistent infection by bovine viral diarrhea virus (BVDV) in 45 clinically suspected persistent infected (PI) calves and 260 apparently healthy calves from Damietta governorate (North) in Egypt during 2016 using enzyme-linked immunosorbent assay (ELISA), serum neutralization test (SNT), immunohistochemistry (IHC), and reverse transcription polymerase chain reaction (RT-PCR). The identification of PI calves was performed after vaccination by CattleMaster® 4. The age of PI calves in the current study was ranged from 2 to 4 months (n = 7) and from 5 to 8 months (n = 38). ELISA revealed the infection in 4 clinically suspected PI calves (57.14%) aged 2 to 4 months. While the infection did not approve in any of the calves within this age using RT-PCR, for calves aged from 5 to 8 months old, ELISA showed the infection in 30 calves (78.94%), and from these calves, the infection was confirmed in 6 calves (15.79%) only using RT-PCR. For apparently clinically healthy animals, 11 transiently infected calves were identified by IHC (positive), SNT (positive), and RT-PCR (negative) at 2 months’ post-vaccination. Collectively, the persistent infection was confirmed in 9 calves (2.95%) out of 305 calves. In summary, our study detected a high prevalence of PI calves with BVDV in Damietta province in Egypt, which highlights the importance of introducing effective prevention and control strategies throughout Egypt to minimize the prevalence of BVDV.

Keywords

Calves Persistent infection Bovine viral diarrhea Egypt 

Notes

Acknowledgments

The authors would like to thank owners of the farms used in this study in Egypt.

Author contribution

Conceived and designed the experiments: SMA, YEE, MAR. Performed the experiments: SMA, MAR, MEB, SMZ. Analyzed the data: SMA, YEE, MAR. Wrote the paper: SMA, MAR. All authors revised and approved manuscript submission.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Statement of animal rights

All Institutional and National Guidelines for the care and use of animals were followed according to the Egyptian Medical Research Ethics Committee (No. 14–126).

References

  1. Amin DM, Emran RM, Aly NM, Farahat EA, Fathi AH (2014) Epidemiology surveillance on bovine viral diarrhea virus and persistently infected animals of cattle and buffaloes in Egypt. Glob Vet 13:856–866Google Scholar
  2. Belak S, Ballagi Pordany A (1991) Bovine viral diarrhea virus infection: rapid diagnosis by the polymerase chain reaction. Arch Virol Suppl 3:181–190CrossRefGoogle Scholar
  3. Brock KV (2004) Strategies for the control and prevention of bovine viral diarrhea virus (BVDV) infections. Vet Microbiol 64:135–144Google Scholar
  4. Cerberey EA, Lee A (1966) SNT for BVD and IBR viruses implying established tissue culture cell lines. Pro. 69th Annual meet., U. S. livestock Saint. Ass. 501Google Scholar
  5. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159CrossRefGoogle Scholar
  6. Cornish TE, Olphen AL, Cavender JL, Edwards JM, Jaeger PT, Vieyra LL et al (2005) Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus. J Vet Diagn Investig 17:110–117CrossRefGoogle Scholar
  7. Drew TW, Yapp F, Paton D (1999) The detection of bovine viral diarrhea virus in bulk milk samples by the use of a single-tube RT-PCR. Vet Microbiol 64:145–154CrossRefGoogle Scholar
  8. Dubovi EJ (1996) Laboratory diagnosis of bovine viral diarrhea virus infections. Vet Med 91:867–872Google Scholar
  9. Dubovi EJ (2013) Laboratory diagnosis of bovine viral diarrhea virus. Biologicals 41:8e13CrossRefGoogle Scholar
  10. El-Bagoury GF, El-Nahas EM, El-Deen SSS, Salem SAH (2014) Detection and genotyping of bovine viral diarrhea virus in cattle sera. Benha Vet Med J 27:348–353Google Scholar
  11. Ferreira GM, Lourens DC, van Vuuren M (2000) The prevalence of bovine viral diarrhea antibodies in selected South African dairy herds, and control of the disease. J S Afr Vet Assoc 71:10–13CrossRefGoogle Scholar
  12. Garoussi MT, Bassami MR, Afshari SE (2007) Detection of bovine viral diarrhea virus using a nested RT-PCR assay in bulk milk samples of dairy cattle herds in a suburb of Mashhad-Iran. Iran J Biotechnol 5:52–55Google Scholar
  13. Goyal SM, Ridpath JF (2005) Bovine viral diarrhea virus, diagnosis, management, and control, 1st (Ed). ISBN 0-8138-0478 -7Google Scholar
  14. Grooms DL, Keilen ED (2002) Screening of neonatal calves for persistent infection with bovine viral diarrhea virus by immunohistochemistry on skin biopsy samples. Clin Diagn Lab Immunol 9:898–900Google Scholar
  15. Hamel AL, Wasylyshen MD, Nayar GPS (1995) Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and one-tube reverse transcription PCR assay. J Clin Microbiol 33:287–291Google Scholar
  16. Heinz FX, Collett MS, Purcell RH, Gould EA, Howard CR et al (2000) Family Flaviviridae. In: van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (eds) Virus taxonomy. Seventh report on International Committee on Taxonomy of Viruses. Academic Press, San Diego, pp 859–878Google Scholar
  17. Hertig C, Pauli U, Zanoni R, Peterhans E (1991) Detection of bovine viral diarrhea (BVD) virus using the polymerase chain reaction. Vet Microbiol 26:65–76CrossRefGoogle Scholar
  18. Hilbe M, Stalder H, Peterhans E, Haessig M, Nussbaumer M, Egli C, Schelp C, Zlinszky K, Ehrensperger F (2007) Comparison of five diagnostic methods for detecting bovine viral diarrhea virus infection in calves. J Vet Diagn Investig 19:28–34CrossRefGoogle Scholar
  19. Houe H (1995) Epidemiology of bovine viral diarrhea. Vet Clin North Am Food Anim Pract 11:521–547CrossRefGoogle Scholar
  20. Kelling CL, Grotelueschen DM, Smith DR, Brodersen BW (2000) Testing and management strategies for effective beef and dairy herd BVDV biosecurity programs. Bov Pract 34:13–22Google Scholar
  21. Kvinesdal BB, Nielsen CM, Poulsen A, Hqjlyng N (1989) Immunofluorescense assay for detection of antibodies to human immunodeficiency virus type 2. J Clin Microbial 27:2502–2504Google Scholar
  22. Moennig V, Houe H, Lindberg A (2005) BVD control in Europe: current status and perspectives. Anim Health Res Rev 6:63–74CrossRefGoogle Scholar
  23. Muñoz-Zanzi CA, Hietala SK, Thurmond MC et al (2003) Quantification, risk factors, and health impact of natural congenital infection with bovine viral diarrhea virus in dairy calves. Am J Vet Res 4:358–365CrossRefGoogle Scholar
  24. Pellerin C, van den Hurk J, Lecomte J, Tussen P (1994) Identification of a new group of bovine viral diarrhea virus strains associated with severe outbreaks and high mortalities. Virology 203:260–268CrossRefGoogle Scholar
  25. Radostitis OM, Gay CC, Hinchcliff KW, Constable PD (2007) Veterinary medicine- a textbook of the diseases of cattle, horses, sheep, pigs and goats, 10th (ed) Saunders El Sevier Printed in Spain 1248–1276Google Scholar
  26. Reed LJ, Muench H (1938) A simple method for estimating 50% end points. Am J Hyg 27:493–497Google Scholar
  27. Ridpath JF, Neill JD (2000) Detection and characterization of genetic recombination in cytopathic type 2 bovine viral diarrhea viruses. J Virol 74:8771–8774CrossRefGoogle Scholar
  28. Ridpath JF, Bolin SR, Katz J (1993) Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5′ noncoding region for detection of bovine viral diarrhea virus. J Clin Microbiol 31:986–989Google Scholar
  29. Schefers JM (2009) Detection, characterization and control of bovine viral diarrhea in dairy herds. Ph.D. thesis, the faculty of graduate school of the University of MinnesotaGoogle Scholar
  30. Yamane D, Kato K, Tohya Y, Akashi H (2008) The relationship between the viral RNA level and upregulation of innate immunity in spleen of cattle persistently infected with bovine viral diarrhea virus. Vet Microbiol 129:69–79CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  2. 2.Veterinary Serum and Vaccine Research InstituteAbbasiaEgypt

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