Comparative Clinical Pathology

, Volume 28, Issue 2, pp 435–445 | Cite as

Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats

  • Samaneh Goorani
  • Mohammad Mahdi ZangenehEmail author
  • Mohammad Kazem Koohi
  • Niloofar Seydi
  • Akram Zangeneh
  • Narges Souri
  • Marziye-Sadat Hosseini
Original Article


Treatment of wounds by ethnomedicinal plants which have fewer side effects than chemical drugs has been on the rise. In this experiment, we evaluated cutaneous wound healing potential of aqueous extract of Falcaria vulgaris in Wistar male rats. DPPH free radical scavenging test was used to examine the antioxidant effect of F. vulgaris aqueous extract, which indicated high antioxidant activity compared to butylated hydroxy toluene (BHT) as the positive control. In our study, after creating the cutaneous wound on the back of the rats, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, treatment with 3% F. vulgaris aqueous extract ointment. The groups were treated for 30 days. For histopathological and biochemical analysis of the cutaneous wound healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at days 10, 20, and 30. The use of F. vulgaris aqueous extract ointment in the treatment groups led to significant decrease (p < 0.05) in the levels of wound area, total cells, lymphocyte, neutrophil, macrophage, and significant enhance (p < 0.05) in the levels of wound contracture, hydroxyproline, hexosamine, fibrocyte, fibroblast, and the rate of fibrocyte to fibroblast as compared to the control and basal ointment groups. According to the results, F. vulgaris aqueous extract ointment can treat the cutaneous wound.


Falcaria vulgaris Aqueous extract Ointment Wound healing potential 


Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Toxicology, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  2. 2.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  3. 3.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran

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