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Assessment of antioxidant and cutaneous wound healing effects of Falcaria vulgaris aqueous extract in Wistar male rats

  • Samaneh Goorani
  • Mohammad Mahdi Zangeneh
  • Mohammad Kazem Koohi
  • Niloofar Seydi
  • Akram Zangeneh
  • Narges Souri
  • Marziye-Sadat Hosseini
Original Article

Abstract

Treatment of wounds by ethnomedicinal plants which have fewer side effects than chemical drugs has been on the rise. In this experiment, we evaluated cutaneous wound healing potential of aqueous extract of Falcaria vulgaris in Wistar male rats. DPPH free radical scavenging test was used to examine the antioxidant effect of F. vulgaris aqueous extract, which indicated high antioxidant activity compared to butylated hydroxy toluene (BHT) as the positive control. In our study, after creating the cutaneous wound on the back of the rats, the animals were randomly divided into four groups; untreated control, treatment with Eucerin ointment, treatment with 3% tetracycline ointment, treatment with 3% F. vulgaris aqueous extract ointment. The groups were treated for 30 days. For histopathological and biochemical analysis of the cutaneous wound healing trend, a 3 × 3 cm section was prepared from all dermal thicknesses at days 10, 20, and 30. The use of F. vulgaris aqueous extract ointment in the treatment groups led to significant decrease (p < 0.05) in the levels of wound area, total cells, lymphocyte, neutrophil, macrophage, and significant enhance (p < 0.05) in the levels of wound contracture, hydroxyproline, hexosamine, fibrocyte, fibroblast, and the rate of fibrocyte to fibroblast as compared to the control and basal ointment groups. According to the results, F. vulgaris aqueous extract ointment can treat the cutaneous wound.

Keywords

Falcaria vulgaris Aqueous extract Ointment Wound healing potential 

Notes

Compliance with ethical standards

Conflict of interests

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.

References

  1. Allahtavakoli M, Arab Bani Asad F, Mahmoudi M, Jafari Naveh H, Tavakolian V, Kamali M, Mahmoudi M, Settaee Mokhtari S (2010) Effect of hydro-alcoholic extract of Artemisia aucheri on healing of skin wound in rat. J Mazand Univ Med Sci 20(77):70–76Google Scholar
  2. Ayyanar M, Ignacimuthu S (2009) Herbal medicines for wound healing among tribal people in Southern India: ethnobotanical and scientific evidences. Int J Appl Res Nat Prod 2(3):29–42Google Scholar
  3. Caetano GF, Fronza M, Leite MN, Gomes A, Frade MAC (2016) Comparison of collagen content in skin wounds evaluated by biochemical assay and by computer-aided histomorphometric analysis. Pharm Biol 54(11):2555–2559PubMedCrossRefGoogle Scholar
  4. Dong Y-L, Fleming RYD, Yan TZ, Herndon DN, Waymack JP (1993) Effect of ibuprofen on the inflammatory response to surgical wounds. J Trauma 35(3):340–343PubMedCrossRefGoogle Scholar
  5. Dwivedi D, Dwivedi M, Malviya S, Singh V (2017) Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Complement Med 7(1):79–85PubMedCrossRefGoogle Scholar
  6. Ebrahimi Monfared K, Rafiee Z, Jafari SM (2012) Phenolic content and antioxidant activity of Falcaria vulgaris extracts. Anal Chem Lett 2(3):159–170CrossRefGoogle Scholar
  7. Fahimi SH, Hajimehdipoor H, Abdollahi M, Mortazavi SA (2015) Burn healing plants in Iranian traditional medicine. Res J Pharmacogn 2(1):53–68Google Scholar
  8. Farzaei MH, Zangeneh MM, Goodarzi N, Zangeneh A (2018) Stereological assessment of nephroprotective effects of Trachyspermum ammi essential oil against carbon tetrachloride-induced nephrotoxicity in mice. Int J Morphol 36(2):750–757CrossRefGoogle Scholar
  9. Foschi D, Trabucchi E, Musazzi M, Castoldi L, Di Mattia D, Radaelli E, Marazzi M, Franzini P, Berlusconi A (1988) The effects of oxygen free radicals on wound healing. Int J Tissue React 10(6):373–379PubMedGoogle Scholar
  10. Geethalakshmi R, Sakravarthi C, Kritika T, Arul Kirubakaran M, Sarada DVL (2013) Evaluation of antioxidant and wound healing potentials of Sphaeranthus amaranthoides Burm.f. Biomed Res Int 2013:607109PubMedPubMedCentralCrossRefGoogle Scholar
  11. Ghasemi-Pirbalouti A, Azizi S, Koohpayeh A (2012) Healing potential of Iranian traditional medicinal plants on burn wounds in alloxan-induced diabetic rats. Rev Bras Farmacogn Braz J Pharmacogn 22(2):397–403CrossRefGoogle Scholar
  12. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263CrossRefGoogle Scholar
  13. Goorani S, Zangeneh MM, Zangeneh A, Poorshamohammad C, Abiari M, Moradi R, Najafi F, Tahvilian R (2018) Study of wound healing potential of Stevia rebaudiana ethanol extract in male rats. Res J Pharmacogn 5(1):23–30Google Scholar
  14. Guo S, DiPietro LA (2010) Factors affecting wound healing. J Dent Res 89(3):219–229PubMedPubMedCentralCrossRefGoogle Scholar
  15. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463CrossRefGoogle Scholar
  16. Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organometal Chem.  https://doi.org/10.1002/aoc.4458 CrossRefGoogle Scholar
  17. Hosseinimehr SJ, Mahmoudzadeh A, Ahmadi A, Ashrafi SA, Shafaghati N, Hedayati N (2011) The radioprotective effect of Zataria multiflora against genotoxicity induced by γ irradiation in human blood lymphocytes. Cancer Biother Radiopharm 26(3):325–329PubMedCrossRefGoogle Scholar
  18. Hunt TK, Hopf HW (1997) Wound healing and wound infection-what surgeons and anaesthesiologists can do? Surg Clin North Am 77(3):587–606PubMedCrossRefGoogle Scholar
  19. Jaberian H, Piri K, Nazari J (2013) Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chem 136(1):237–244PubMedCrossRefGoogle Scholar
  20. Jarrahi M, Emami Abarghuee M (2008) Effect of hydro-alcoholic extract of Matricaria chamomilla L on burned wound healing in rat. J Gorgan Uni Med Sci 10:22–26Google Scholar
  21. Jarrahi M, Zahedi M, Ajorlu M (2009) Effect of Aloe barbadensis Miller on skin wound healing in rat. J Gorgan Uni Med Sci 11:13–17Google Scholar
  22. Jivad N, Bahmani M (2006) A review of important medicinal plants native to Iran effective on recovery from peptic ulcer. Der Pharm Lett 8(2):347–352Google Scholar
  23. Johnston DE (1990) Wound healing in skin, plastic and reconstructive surgery. Vet Clinic North Am 20(1):1–45CrossRefGoogle Scholar
  24. Khan MN, Naqui AH (2006) Antiseptics, iodine, povidone iodine and traumatic wound cleansing. J Tissue Viability 16(4):6–10PubMedCrossRefGoogle Scholar
  25. Khanahmadi M, Shahrezaei F (2008) Review and identify the chemical constituents of volatile oils of Falcaria vulgaris Bernh. J Med Plant 6(3):52–57Google Scholar
  26. Koh TJ, DiPietro LA (2011) Inflammation and wound healing: the role of the macrophage. Expert Rev Mol Med 13:e23PubMedPubMedCentralCrossRefGoogle Scholar
  27. Kramer SA (1999) Effect of povidone-iodine on wound healing: a review. J Cardiovasc Nurs 17(1):17–23Google Scholar
  28. Lazarus GS, Cooper DM, Knighton DR, Margolis DJ, Pecoraro RE, Rodeheaver G, Robson MC (1994) Definitions and guidelines for assessment of wounds and evaluation of healing. Arch Dermatol 130(4):489–493PubMedCrossRefGoogle Scholar
  29. McCauley RL, Li YY, Poole B, Evans MJ, Robson MC, Heggers JP, Herndon DN (1992) Differential inhibition of human basal keratinocyte growth to silver sulfadiazine and mefenide acetate. J Surg Res 52(3):276–285PubMedCrossRefGoogle Scholar
  30. Moradi R, Hajialiani M, Salmani S, Almasi M, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Com Cli Path.  https://doi.org/10.1007/s00580-018-2834-y
  31. Nayak BS, Isitor G, Davis EM, Pillai GK (2007) The evidence based wound healing activity of Lawsonia inermis Linn. Phytother Res 21(9):827–831PubMedCrossRefGoogle Scholar
  32. Oryan A, Tabatabaiei Naieni A, Moshiri A, Mohammadalipoor A, Tabandeh MR (2012) Modulation of cutaneous wound healing by Silymarin in rats. J Wound Care 21(9):457–464PubMedCrossRefGoogle Scholar
  33. Phillips GD, Whitehe RA, Kinghton DR (1991) Initiation and pattern of angiogenesis in wound healing in the rats. Am J Anat 192(3):257–262PubMedCrossRefGoogle Scholar
  34. Robards K, Prenzler PD, Tucker G, Swatsitang P, Glover W (1999) Phenolic compounds and their role in oxidative processes in fruits. Food Chem 66(4):401–436CrossRefGoogle Scholar
  35. Ruszczak Z, Schwartz RA (2000) Modern aspects of wound healing: an update. Dermatol Surg 26(3):219–229PubMedCrossRefGoogle Scholar
  36. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Pl 21(1):164–174CrossRefGoogle Scholar
  37. Sewall GK, Robertson KM, Connor NP, Heisey DM, Hartig GK (2003) Effect of topical mitomycin on skin wound contraction. Arch Facial Plast Surg 5(1):59–62PubMedCrossRefGoogle Scholar
  38. Shakibaie D, Pasharavesh L, Khoshboo S, Kaboodi B (2007) The effect of the “Falcaria vulgaris” on deep skin wound remodeling time and skin tension power in rats. J Kermanshah Univ Med Sci 10(3):187–194Google Scholar
  39. Sharafzadeh S, Alizadeh O (2012) Some medicinal plants cultivated in Iran. J Appl Pharm Sci 2(1):134–137Google Scholar
  40. Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A (2017) Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci 69(3):535–543CrossRefGoogle Scholar
  41. Singer AD, Dagum AB (2008) Current management of acute cutaneous wounds. N Engl J Med 350:1037–1046CrossRefGoogle Scholar
  42. Souba WW, Wilmore D (1999) Diet and nutrition in case of the patient with surgery. Trauma and sepsis, 9th edn. Williams and Wilkins, Baltimore, pp 1589–1618Google Scholar
  43. Stavrou D (2008) Neovascularisation in wound healing. J Wound Care 17(7):298–302PubMedCrossRefGoogle Scholar
  44. Thomas GW, Rael TL, Bar-Or R, Shimonkevitz R, Mains CW, Slone DS, Craun ML, Bar-Or D (2009) Mechanisms of delayed wound healing by commonly used antiseptics. J Trauma 66(1):82–91PubMedCrossRefGoogle Scholar
  45. Townsend CM (2001) Sabiston textbook of surgery, 16th edn. Oxford University Prass, New York, pp 85–91Google Scholar
  46. Zangeneh MM, Salmani S, Zangeneh A, Bahrami E, Almasi M (2018a) Antiulcer activity of aqueous extract of leaves of Mentha piperita in Wistar rats. Com Cli Path.  https://doi.org/10.1007/s00580-018-2827-x
  47. Zangeneh MM, Salmani S, Zangeneh A, Khedri R, Zarei MS (2018b) Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet. Com Cli Path.  https://doi.org/10.1007/s00580-018-2828-9
  48. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2018c) Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Biol Sci.  https://doi.org/10.2298/ABS180222027Z
  49. Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R, Zhaleh H, Amiri-Paryan A, Bahrami E (2018d) Hepatoprotective and hematoprotective effects of Falcaria vulgaris aqueous extract against CCl4-induced hepatic injury in mice. Comp Clin Path 27(5):1359–1365CrossRefGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Toxicology, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  2. 2.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  3. 3.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran

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