Comparative Clinical Pathology

, Volume 28, Issue 3, pp 603–611 | Cite as

The effect of lipotropic agents on the nutritional induction of fatty liver syndrome in broilers

  • Emad A. Hashish
  • Doaa I. A. MostafaEmail author
  • Ghada M. El Khder
Original Article


One of the major metabolic disorders in poultry industry is the incidence of fatty deposits in the hepatic tissue. The high-energy diets are accompanied with fatty liver and damaging effect on the hepatic tissue. This study hypothesized that the application of lipotropic agents may help broiler chicks to efficiently utilize the high-energy diets. A total of 100-day-old broiler chicks were randomly divided into five equal groups; negative control, positive control (fed on high-energy diet), and three groups fed on high-energy diet with supplemental lipotropic agents (100 mg/kg b.wt. carnitine as l-carnitine or 1000 mg/kg b.wt. choline as choline chloride or combination of both). Average body weight (BW) and daily weight gain (BWG) were recorded during the 42-day experimental period. Alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activity, beside the serum level of total protein, albumin, globulin and A/G ratio, lipogram, and histopathological examination were carried out. The BW and BWG were decreased with an increase in ALT and ALP activity in the positive control group. Proteinogram was not affected; meanwhile, a significant increase in triglyceride (TG) was observed. Dietary supplementation of these lipotropic agents (carnitine and choline) improved all these parameters. Choline and carnitine co-treatment showed better effect than each one individually. Our histopathological examination confirms the results. Interestingly, the modulation of lipotropic agents for co-administration considers a new mechanism with a pathway key to overcome on hepatic fat accumulation.


Broilers Choline Carnitine Fatty liver 


  1. Arslan C, Citil M, Saatci M (2003) Effects of L-carnitine administration on growth performance, carcass traits, blood serum parameters and abdominal fatty acid composition of ducks. Arch Anim Nutr 57:381–388CrossRefGoogle Scholar
  2. Bancroft JD, Floyd AD, Suvarna SK (2013) Bancroft\’s theory and practice of histological techniquesGoogle Scholar
  3. Bremer J (1997) The role of carnitine in cell metabolism. In: Carnitine today. Springer, Berlin, pp 1–37Google Scholar
  4. Browning JD, Horton JD (2004) Molecular mediators of hepatic steatosis and liver injury. J Clin Invest 114:147–152CrossRefPubMedPubMedCentralGoogle Scholar
  5. Buyse J, Janssens G, Decuypere E (2001) The effects of dietary L-carnitine supplementation on the performance, organ weights and circulating hormone and metabolite concentrations of broiler chickens reared under a normal or low temperature schedule. Br Poult Sci 42:230–241CrossRefPubMedGoogle Scholar
  6. Caballero F, Fernandez A, Matias N, Martinez L, Fucho R, Elena M, Caballeria J, Morales A, Fernandez-Checa JC, Garcia-Ruiz C (2010) Specific contribution of methionine and choline in nutritional nonalcoholic steatohepatitis: impact on mitochondrial S-adenosyl-L-methionine and glutathione. J Biol Chem 285:18528–18536CrossRefPubMedPubMedCentralGoogle Scholar
  7. Choi Y, Ahn H, Lee B, Oh S, An B, Kang C (2012) Nutritional and hormonal induction of fatty liver syndrome and effects of dietary lipotropic factors in egg-type male chicks. Asian Australas J Anim Sci 25:1145–1152CrossRefPubMedPubMedCentralGoogle Scholar
  8. da Silva RP, Kelly KB, Lewis ED, Leonard KA, Goruk S, Curtis JM, Vine DF, Proctor SD, Field CJ, Jacobs RL (2015) Choline deficiency impairs intestinal lipid metabolism in the lactating rat. J Nutr Biochem 26:1077–1083CrossRefPubMedGoogle Scholar
  9. Dawson PA, Lan T, Rao A (2009) Bile acid transporters. J Lipid Res 50:2340–2357CrossRefPubMedPubMedCentralGoogle Scholar
  10. Feldsine P, Abeyta C, Andrews WH (2002) AOAC international methods committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis. J AOAC Int 85:1187–1200PubMedGoogle Scholar
  11. Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–502Google Scholar
  12. Hossininezhad MM, Irani M, Seidavi A (2011) Dietary effects of L-carnitine supplement on performance and blood parameters of broiler chickens. J Food Agric Environ 9:475–481Google Scholar
  13. Izzo C, Grillo F, Murador E (1981) Improved method for determination of high-density-lipoprotein cholesterol I. Isolation of high-density lipoproteins by use of polyethylene glycol 6000. Clin Chem 27:371–374PubMedGoogle Scholar
  14. Kaplan A et al. (1984) Triglycerides. Clin. Chem. The C.V. Mosby St. Louis Toronto Priceton. 1194–1206Google Scholar
  15. Kheiri F, Pourreza J, Ebrahimnezhad Y, Nazeradl K, Haji-abadi SMAJ (2011) Effects of supplemental ractopamine and L-carnitine on growth performance, blood biochemical parameters and carcass traits of male broiler chicks. Afr J Biotechnol 10:15450–15455CrossRefGoogle Scholar
  16. Khoshkhoo PH, Azad GA, Ila N, Moayer F, and Nayeri HD (2006) Effect of dietary L-carnitine supplementation on overall performance, carcass traits, serum components and immune response in broiler chicken. In: EPC 2006-12th European Poultry Conference, Verona, Italy, 10–14 September, 2006. World’s Poult Sci Association (WPSA)Google Scholar
  17. Krohn RI (2005) The colorimetric detection and quantitation of total protein. Current protocols in toxicology/editorial board, Mahin D. Maines Appendix 3:A 3I 1–28Google Scholar
  18. Li Z, Agellon LB, Vance DE (2005) Phosphatidylcholine homeostasis and liver failure. J Biol Chem 280:37798–37802CrossRefGoogle Scholar
  19. Lukicheva TI, Pivovarova S, Doroguntseva (1987) II [Determination of serum albumin by a colorimetric micromethod using bromcresol purple]. Laboratornoe delo 3–6Google Scholar
  20. Murali P, George S, Ally K, Dipu M (2015) Effect of L-carnitine supplementation on growth performance, nutrient utilization, and nitrogen balance of broilers fed with animal fat. Vet World 8:482–486CrossRefPubMedPubMedCentralGoogle Scholar
  21. Nauck M, Warnick GR, Rifai N (2002) Methods for measurement of LDL-cholesterol: a critical assessment of direct measurement by homogeneous assays versus calculation. Clin Chem 48:236–254PubMedGoogle Scholar
  22. Noga AA, Vance DE (2003) A gender-specific role for phosphatidylethanolamine N-methyltransferase-derived phosphatidylcholine in the regulation of plasma high density and very low density lipoproteins in mice. J Biol Chem 278:21851–21859CrossRefPubMedGoogle Scholar
  23. Omene JA, Glew RH, Baig HA, Robinson DB, Brock W, Chambers JP (1981) Determination of serum acid and alkaline phosphatase using 4-methylumbelliferyl phosphate. Afr J Med Med Sci 10:9–18PubMedGoogle Scholar
  24. Rabie MH, Szilágyi M (1998) Effects of L-carnitine supplementation of diets differing in energy levels on performance, abdominal fat content, and yield and composition of edible meat of broilers. Br J Nutr 80:391–400CrossRefPubMedGoogle Scholar
  25. Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–63CrossRefPubMedGoogle Scholar
  26. Swain BK, Johri TS (2000) Effect of supplemental methionine, choline and their combinations on the performance and immune response of broilers. Br Poult Sci 41:83–88CrossRefPubMedGoogle Scholar
  27. Tamhane AC, Dunlop DD (2000) Statistic and data analysis from elementary to intermediate. Upper Saddle River, USAGoogle Scholar
  28. Taylor RP, Broccoli AV, Grisham CM (1978) Enzymatic and colorimetric determination of total serum cholesterol. An undergraduate biochemistry laboratory experiment. J Chem Educ 55:63–64CrossRefPubMedGoogle Scholar
  29. Trott KA, Giannitti F, Rimoldi G, Hill A, Woods L, Barr B, Anderson M, Mete A (2014) Fatty liver hemorrhagic syndrome in the backyard chicken: a retrospective histopathologic case series. Vet Pathol 51:787–795CrossRefPubMedGoogle Scholar
  30. Underwood BA (1984) Vitamin A in animal and human nutrition. The Retinoids 1:281–392CrossRefGoogle Scholar
  31. Veenema K, Solis C, Li R, Wang W, Maletz CV, Abratte CM, Caudill MA (2008) Adequate intake levels of choline are sufficient for preventing elevations in serum markers of liver dysfunction in Mexican American men but are not optimal for minimizing plasma total homocysteine increases after a methionine load. Am J Clin Nutr 88:685–692CrossRefPubMedPubMedCentralGoogle Scholar
  32. Wall BT, Stephens FB, Constantin-Teodosiu D, Marimuthu K, Macdonald IA, Greenhaff PL (2011) Chronic oral ingestion of l-carnitine and carbohydrate increases muscle carnitine content and alters muscle fuel metabolism during exercise in humans. J Physiol 589:963–973CrossRefPubMedPubMedCentralGoogle Scholar
  33. Whitehead CC (1979) Nutritional and metabolic aspects of fatty liver disease in poultry. Tijdschrift diergeneeskunde 104(suppl):150–157Google Scholar
  34. Williams EA, Gebhardt BM, Morton B, Newberne PM (1979) Effects of early marginal methionine-choline deprivation on the development of the immune system in the rat. Am J Clin Nutr 32:1214–1223CrossRefPubMedGoogle Scholar
  35. Zeisel SH (2006) Choline: critical role during fetal development and dietary requirements in adults. Annu Rev Nutr 26:229–250CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Pathology, Faculty of Veterinary MedicineZagazig UniversityZagazigEgypt
  2. 2.Department of Clinical PathologyAnimal Health Research Institute, Zagazig BranchZagazigEgypt
  3. 3.Department of Chemistry, Toxicology and Nutritional DifficencyAnimal Health Research Institute, Zagazig BranchZagazigEgypt

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