Morphological and molecular identification of Acanthocheilonema reconditum in a canine
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Acanthocheilonema reconditum (A. reconditum) is a nematode with low pathogenicity that causes canine filariasis, without clinical signs. This filarid produces microfilariae that circulate in the blood of their natural hosts. Microfilariae of A. reconditum can be easily confused with microfilariae of Dirofilaria immitis (D. immitis) which causes a severe disease in dogs. The aim of this article is to describe a case of A. reconditum infection in a dog, diagnosed by fine needle aspiration cytology (FNAC) of two cutaneous nodules, blood smear examination, and further confirmed by molecular assay (PCR). This report alerts veterinarians to the presence of A. reconditum in the region of Santa Maria, Rio Grande do Sul, Brazil, and highlights the importance of performing the differentiation of microfilaria species. In addition, it demonstrates the importance of fine needle aspiration cytology as a diagnostic tool.
KeywordsDipetalonema reconditum Filarial nematodes Microfilariae Dog
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
Not applicable. To report this case, we had the consent of the guardian of the animal and the veterinarians who participated in the case.
- Hashem MA, Badawy AII (2008) Blood cellular and biochemical studies on filariasis of dogs. Res J Anim Sci 2(5):128–134Google Scholar
- Lindemann BA, Mccall JW (1893) Microfilaricidal activity of ivermectin against Dipetalonema reconditum. J Vet Pharmacol Ther 6:74–76Google Scholar
- Nelson RW, Couto CG (eds) (2010) Small animal internal medicine. 4. ed. Elsevier, São Paulo, pp 169–183Google Scholar
- Stockham SL, Scott MA (eds) (2008) Fundamentals of veterinary clinical pathology. 2 ed. Wiley-Blackwell, Ames, pp 303–552Google Scholar