The aqueous extract of Glycyrrhiza glabra effectively prevents induced gastroduodenal ulcers: experimental study on Wistar rats
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The recent studies have indicated the properties of ethnomedicinal plants in the prevention, control, and treatment of gastroduodenal ulcers. Glycyrrhiza glabra has been used in Iranian traditional medicine as a therapeutic supplement. The aim of our research was to survey the preventive property of aqueous extract of G. glabra leaf on ibuprofen-induced gastroduodenal ulcers by investigating the biochemical, hematological, immunological, and microscopic approaches in rats. In this study, 60 rats were used. The animals were randomly divided into six subgroups, including negative healthy control, untreated negative control, the positive control receiving omeprazole 60 mg/kg, and three groups receiving the G. glabra aqueous extract at 20 mg/kg, 60 mg/kg, and 180 mg/kg concentrations. After 14 days, gastroduodenal ulcers were caused by ibuprofen 400 mg/kg. Four hours after oral administration of ibuprofen, the rats were sacrificed and blood, stomach, and duodenum samples of them collected for analysis of biochemical, hematological, immunological, and microscopic parameters. The data were analyzed by SPSS 21 software. All doses of G. glabra could significantly (p ≤ 0.05) reduce the raised levels of ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, urea, creatinine, IL-1, IL-6, IL-12, IL-18, IFN-γ, and TNF-α and increase HDL, total protein, albumin, WBC, platelet, RBC, IL-4, IL-5, IL-10, IL-13, and IFN-α as compared to the untreated group. Also, aqueous extract of G. glabra prevented significantly (p ≤ 0.05) gastroduodenal ulcers as compared to the untreated group. In conclusion, the obtained results indicated the hepatoprotective, nephroprotective, hematoprotective, immunoprotective, and gastroduodenal protective properties of G. glabra aqueous extract.
KeywordsGlycyrrhiza glabra Hepatoprotective Nephroprotective Hematoprotective Immunoprotective Gastroduodenal protective
The authors would like to thank the Kermanshah University of Medical Sciences for the financial support.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All institutional and national guidelines for the care and use of laboratory animals were followed.
- Abd-Al-Sattar Sadiq Layl L (2016) Hepatoprotective effect of Glycyrrhiza glabra L. extracts against carbon tetrachloride-induced acute liver damage in rats. Int J Vet Sci 1(1):1–8Google Scholar
- Anderson MD, Piper SE, Swan GE (2005) Nonsteroidal anti-inflammatory drug use in South Africa and possible effects on vultures. S Afr J Anim Sci 101:112–114Google Scholar
- Anil K, Jyotsna D (2012) Review on Glycyrrhiza glabra. J Pharm Sci Innov 1(2):1–4Google Scholar
- Boshra SA, Hussein MA (2014) The protective role of colchicine on diclofenac sodium induced hepatorenal toxicity in albino rats model. Int J Pharm Sci Res 5(12):5136–5144Google Scholar
- Bushra R, Aslam N (2010) An overview of clinical pharmacology of ibuprofen. Oman Med J 25(3):155–161Google Scholar
- Dehghani-Tafti L, Shariatpanahi SM, Mahdavi-Damghani M, Javadi B (2017) Traditional Persian topical medications for gastrointestinal diseases. Iran J Basic Med Sci 20(3):222–241Google Scholar
- Dwivedi V, Mishra J, Shrivastava A (2015) Efficacy study of livartho against paracetamol induced hepatotoxicity in adult Sprague Dawley rats. J Drug Metab Toxicol 5(6):175–181Google Scholar
- El-Maddawy ZKH, El-Ashmawy IM (2013) Hepato-renal and haematological effects of diclofenac sodium in rats. Global J Pharmacol 7(2):123–132Google Scholar
- Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organometal Chem 32. https://doi.org/10.1002/aoc.4458
- Hörl WH (2010) Non-steroidal anti-inflammatory drugs and the kidney. Pharma 3(7):2291–2321Google Scholar
- Jung JC, Lee YH, Kim SH, Kim KJ, Kim KM, Oh S, Jung YS (2016) Hepatoprotective effect of licorice, the root of Glycyrrhiza uralensis Fischer, in alcohol-induced fatty liver disease. BMC Complement Altern Med 16. https://doi.org/10.1186/s12906-016-0997-0
- Li YJ, Chen J, Li Y, Li Q, Zheng YF, Fu Y, Li P (2011) Screening and characterization of natural antioxidants in four Glycyrrhiza species by liquid chromatography coupled with electrospray ionization quadrupole time-offlight tandem mass spectrometry. J Chromatogr A 11:8181–8191CrossRefGoogle Scholar
- Montoro P, Maldini M, Russo M, Postorino S, Piacente S, Pizza C (2011) Metabolic profiling of roots of liquorice (Glycyrrhiza glabra) from different geographical areas by ESI/MS/MS and determination of major metabolites by LC-ESI/MS and LC-ESI/MS/MS. J Pharm Biomed Anal 54(3):535–544CrossRefGoogle Scholar
- Moradi R, Hajialiani M, Salmani S, Almasi M, Zangeneh A, Zangeneh MM (2018) Effect of aqueous extract of Allium saralicum R.M. Fritsch on fatty liver induced by high-fat diet in Wistar rats. Com Cli Pathol. https://doi.org/10.1007/s00580-018-2834-y
- Reynolds EF (1982) Aspirin and similar analgesic and anti-inflammatory agents. In: Martindale. The extra pharmacopoeia, 28th edn. Pharmaceutical, London, pp 234–282Google Scholar
- Samadnejad AZ, Mehrvarz S, Naeini SA, Tanideh N (2012) Healing effect of licorice extract in acetic acid-induced ulcerative colitis in rat. Res Pharm Sci 7(5):837–845Google Scholar
- Shafi M, Garg UK, Saqib N, Baba OK, Farid BD, Wali A (2012) Haemato-biochemical studies on diclofenac, ibuprofen and nimesulide induced toxicity in broilers. Nat Environ Pollut Technol 11(4):649–652Google Scholar
- Sonnenberg A, Chicarro ML (1991) Peptic ulcer in the United States. Rev Esp Enferm Dig 79(5):341–349Google Scholar
- Swan SK, Rudy DW, Lasseter KC, Ryan CF, Buechel KL, Lambrecht LJ, Pinto MB, Dilzer SC, Obrda O, Sundblad KJ, Gumbs CP, Ebel DL, Quan H, Larson PJ, Schwartz JI, Musliner TA, Gertz BJ, Brater DC, Yao SL (2000) Effect of cyclooxygenase-2 inhibition on renal function in elderly persons receiving a low-salt diet. A randomized, controlled trial. Ann Intern Med 133(1):1–9CrossRefGoogle Scholar
- Yadeghari M, Khazaei M (2006) Repairing effect of hydroalcoholic extract of aerial part of Facaria vulgaris on aspirin- induced gastric ulcers in rat. Behbood l0(3):195–203Google Scholar
- Yashin A, Yashin Y, Xia X, Nemzer B (2017) Antioxidant activity of spices and their impact on human health: a review. Antioxidants 6. https://doi.org/10.3390/antiox6030070
- Zangeneh MM, Salmani S, Zangeneh A, Bahrami E, Almasi M (2018a) Antiulcer activity of aqueous extract of leaves of Mentha piperita in Wistar rats. Comp Clin Pathol. https://doi.org/10.1007/s00580-018-2827-x
- Zangeneh MM, Salmani S, Zangeneh A, Khedri R, Zarei MS (2018b) Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet. Comp Clin Pathol. https://doi.org/10.1007/s00580-018-2828-9