Comparative Clinical Pathology

, Volume 28, Issue 2, pp 541–547 | Cite as

Protective effect of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats

  • Ghodrat EbadimanasEmail author
Original Article


Protective effects of royal jelly on ofloxacin in male rat reproductive system are not well-known. This study was designed to determine the protective effects of royal jelly on the DNA integrity of sperms and early in vitro embryonic development in ofloxacin treated rats. In this study, 32 adult male rats were used and randomly allocated into 4 groups, each consisted of eight rats and dosed daily for 28 days by oral gavage: control—distilled water (216 mg/kg BW); ofloxacin group—ofloxacin (216 mg/kg BW); royal jelly group—royal jelly (100 mg/kg BW); royal jelly with ofloxacin group—216 mg/kg BW ofloxacin and 100 mg/kg BW royal jelly. The results showed that sperm viability, sperm population, sperm maturation, DNA maturity, hatching, IVF, GSH, and blastocyst significantly decreased (P < 0.05) in the ofloxacin group compared to the control. However, ofloxacin only caused significant increases (P < 0.05) in testis weight, immature sperm, and DNA damage of sperm compared to control group. In the ofloxacin with royal jelly group, there were not any important alterations detected and all sperm parameter returned to normal range. Royal jelly has protective effects on the DNA maturity of sperms and early in vitro embryonic development in ofloxacin treated rats.


Royal jelly Protective Ofloxacin Development Rats 


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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Basic SciencesFarhangian UniversityTehranIran

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