Comparative Clinical Pathology

, Volume 28, Issue 5, pp 1197–1203 | Cite as

Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet

  • Mohammad Mahdi ZangenehEmail author
  • Saman Salmani
  • Akram Zangeneh
  • Reza Khedri
  • Mohammad Saeid Zarei
Original Article


The fatty liver disease is one of the most usual metabolic diseases among people around the world. Appropriate strategies for treating this disease are prioritized by each country. The purpose of the new research was to survey the therapeutical property of aqueous extract of Tragopogon graminifolius on the high-fat diet-induced fatty liver disease in Wistar male rats. In this study, 60 rats were used. A total of ten rats were chosen as the negative control, and the rest of them were treated with a high-fat diet for 4 months. Then, the animals were randomly divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the aqueous extract of T. graminifolius at 20, 40, 80, and 160 mg/kg concentrations. After 2 months, the rats were sacrificed, and the blood and liver samples of them were collected for biochemical and histopathological parameter analysis. All doses of T. graminifolius could significantly (p ≤ 0.05) decrease the raised levels of ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, and glucose and increased HDL, total protein, albumin, SOD, and CAT as compared to the untreated group. Also, aqueous extract of T. graminifolius decreased the degree of hepatic steatosis as compared to the untreated group. In conclusion, the obtained results demonstrated the hepatoprotective effect of T. graminifolius aqueous extract.


Tragopogon graminifolius Aqueous extract Hepatoprotective effect Fatty liver High-fat diet 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.


  1. Abei H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York, pp 673–684CrossRefGoogle Scholar
  2. Bosisio E, Benelli C, Pirola O (1992) Effect of the flavanolignans of Silybum marianum L. on lipid peroxidation in rat liver microsomes and freshly isolated hepatocytes. Pharmacol Res 25(2):147–154CrossRefPubMedGoogle Scholar
  3. Bundy R, Walker AF, Middleton RW, Wallis C, Simpson HC (2008) Artichoke leaf extract (Cynara scolymus) reduces plasma cholesterol in otherwise healthy hypercholesterolemic adults: a randomized, double blind placebo controlled trial. Phytomedicine 15(9):668–675CrossRefPubMedGoogle Scholar
  4. Day CP (2006) From fat to inflammation. Gastroenterol 130:207–210CrossRefGoogle Scholar
  5. Day CP (2011) Non-alcoholic fatty liver disease: a massive problem. Clin Med 11(2):176–178CrossRefGoogle Scholar
  6. Farzaei MH, Khanavi M, Moghaddam G, Dolatshahi F, Rahimi R, Shams-Ardekani MR, Amin G, Hajimahmoodi M (2014a) Standardization of Tragopogon graminifoliusDC. Extractbasedonphenolic compounds and antioxidant activity. J Chem. CrossRefGoogle Scholar
  7. Farzaei MH, Rahimi R, Attar F, Siavoshi F, Saniee P, Hajimahmoodi M, Mirnezami T, Khanavi M (2014b) Chemical composition, antioxidant and antimicrobial activity of essential oil and extracts of Tragopogon graminifolius, a medicinal herb from Iran. Nat Prod Commun 9(1):121–124PubMedGoogle Scholar
  8. Farzaei MH, Ghasemi-Niri SF, Abdolghafari AH, Baeeri M, Khanavi M, Navaei-Nigjeh M, Abdollahi M, Rahimi R (2015) Biochemical and histopathological evidence on the beneficial effects of Tragopogon graminifolius in TNBS-induced colitis. Pharm Biol 53(3):429–436CrossRefPubMedGoogle Scholar
  9. Farzaei MH, Zangeneh MM, Goodarzi N, Zangeneh A (2018) Stereological assessment of nephroprotective effects of Trachyspermum ammi essential oil against carbon tetrachloride-induced nephrotoxicity in mice. Int J Morphol 36(2):750–757CrossRefGoogle Scholar
  10. Flora K, Hahn M, Rosen H, Benner K (1998) Milk thistle (Silybum marianum) for the therapy of liver disease. Am J Gastroenterol 93(2):139–143CrossRefGoogle Scholar
  11. Ganz M, Csak T, Szabo G (2014) High fat diet feeding results in gender specific steatohepatitis and inflammasome activation. World J Gastroenterol 20:8525–8534CrossRefPubMedPubMedCentralGoogle Scholar
  12. Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A (2017) Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci 23(4):256–263CrossRefGoogle Scholar
  13. Haga Y, Kanda T, Sasaki R, Nakamura M, Nakamoto S, Yokosuka O (2015) Nonalcoholic fatty liver disease and hepatic cirrhosis: comparison with viral hepatitis-associated steatosis. World J Gastroenterol 21(46):12989–12995CrossRefPubMedPubMedCentralGoogle Scholar
  14. Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R (2017) Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol 26(2):455–463CrossRefGoogle Scholar
  15. Hamelian M, Zangeneh MM, Amisama A, Varmira K, Veisi H (2018) Green synthesis of silver nanoparticles using Thymus kotschyanus extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Appl Organomet Chem. CrossRefGoogle Scholar
  16. Hemmati M, Zohoori E, Mehrpour O, Karamian M, Asghari S, Zarban A, Nasouti R (2015) Anti-atherogenic potential of jujube, saffron and barberry: anti-diabetic and antioxidant actions. EXCLI J 14:908–915PubMedPubMedCentralGoogle Scholar
  17. Jacobs BP, Dennehy C, Ramirez G, Sapp J, Lawrence VA (2002) Milk thistle for the treatment of liver disease: a systematic review and meta-analysis. Am J Med 113(6):506–515CrossRefPubMedGoogle Scholar
  18. Lee TY, Chang HH, Lo WC, Lin HC (2010) Alleviation of hepatic oxidative stress by Chinese herbal medicine Yin-Chen-Hao-Tang in obese mice with steatosis. Int J Mol Med 25(6):837–844CrossRefPubMedGoogle Scholar
  19. Martin JP, Dailey M, Sugarman E (1987) Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255(2):329–336CrossRefPubMedGoogle Scholar
  20. Mohammadifar M, Behnam M, Talaei SA, Khamechian T, Mehran M, Taghizadeh M (2018) Evaluation effect of Silybum Marianum, Cynara scolymus L and Ziziphus jujube Mill combination extract on nonalcoholic fatty liver in rats. Iran J Endocrinol Metab 19(6):410–418Google Scholar
  21. Nazni P, Vijayakumar TP, Alagianambi P, Amirthaveni M (2006) Hypoglycemic and hypolipidemic effect of Cynara scolymus among selected type 2 diabetic individuals. Pak J Nutr 5(1):147–151Google Scholar
  22. Sabzghabaee AM, Khayam I, Kelishadi R, Ghannadi A, Soltani R, Badri S, Shirani S (2013) Effect of Zizyphus jujuba fruits on dyslipidemia in obese adolescents: a triple-masked randomized controlled clinical trial. Med Arch 67(3):156–159CrossRefPubMedGoogle Scholar
  23. Sadeghi A, Bahramsoltani R, Rahimi R, Farzaei MH, Farzaei F, Siavosh-Haghighi ZM, Abdollahi M (2018) Biochemical and histopathological evidence on beneficial effects of standardized extract from Tragopogon graminifolius as a dietary supplement in fatty liver: role of oxidative stress. J Diet Suppl 15(2):197–206CrossRefPubMedGoogle Scholar
  24. Sayyedrostami T, Pournaghi P, Ebrahimi Vosta-Kalaeea S, Zangeneh MM (2018) Evaluation of the wound healing activity of Chenopodium botrys leaves essential oil in rats (a short-term study). J Essent Oil Bear Plants 21(1):164–174CrossRefGoogle Scholar
  25. Shaker E, Mahmoud H, Mnaa S (2010) Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food Chem Toxicol 48(3):803–806CrossRefPubMedGoogle Scholar
  26. Tamayo C, Diamond S (2007) Review of clinical trials evaluating safety and efficacy of milk thistle (Silybum marianum [L.] Gaertn.). Integr Cancer Ther 6(2):146–157CrossRefPubMedGoogle Scholar
  27. Tenkerian C, El-Sibai M, Daher CF, Mroueh M (2015) Hepatoprotective, antioxidant, and anticancer effects of the Tragopogon porrifolius methanolic extract. Evid Based Complement Alternat Med 2015:1–10. CrossRefGoogle Scholar
  28. Xiao J, Fai So K, Liong EC, Tipoe GL (2013) Recent advances in the herbal treatment of non-alcoholic fatty liver disease. J Tradit Complement Med 3(2):88–94CrossRefPubMedPubMedCentralGoogle Scholar
  29. Yao H, Qiao YJ, Zhao YL, Tao XF, Xu LN, Yin LH, Qi Y, Peng JY (2016) Herbal medicines and nonalcoholic fatty liver disease. World J Gastroenterol 22(30):6890–6905CrossRefPubMedPubMedCentralGoogle Scholar
  30. Zeeni N, Daher CF, Saab L, Mroueh M (2014) Tragopogon porrifolius improves serum lipid profile and increases short-term satiety in rats. Appetite 72:1–7CrossRefPubMedGoogle Scholar
  31. Zhaleh M, Sohrabi N, Zangeneh MM, Zangeneh A, Moradi R, Zhaleh H (2018) Chemical composition and antibacterial effects of essential oil of Rhus coriaria fruits in the west of Iran (Kermanshah). J Essent Oil Bear Plants 21(2):493–501CrossRefGoogle Scholar
  32. Zhang R, Yu Y, Hu S, Zhang J, Yang H, Han B, Cheng Y, Luo X (2016) Sesamin ameliorates hepatic steatosis and inflammation in rats on a high-fat diet via LXR5 and PPAR5. Nutr Res 36(9):1022–1030CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  2. 2.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran

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