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Comparative Clinical Pathology

, Volume 28, Issue 5, pp 1197–1203 | Cite as

Histopathological and biochemical effects of aqueous extract of Tragopogon graminifolius on the liver tissues of Wistar rats fed with high-fat diet

  • Mohammad Mahdi ZangenehEmail author
  • Saman Salmani
  • Akram Zangeneh
  • Reza Khedri
  • Mohammad Saeid Zarei
Original Article

Abstract

The fatty liver disease is one of the most usual metabolic diseases among people around the world. Appropriate strategies for treating this disease are prioritized by each country. The purpose of the new research was to survey the therapeutical property of aqueous extract of Tragopogon graminifolius on the high-fat diet-induced fatty liver disease in Wistar male rats. In this study, 60 rats were used. A total of ten rats were chosen as the negative control, and the rest of them were treated with a high-fat diet for 4 months. Then, the animals were randomly divided into six subgroups, including negative healthy control, untreated negative control, and four groups receiving the aqueous extract of T. graminifolius at 20, 40, 80, and 160 mg/kg concentrations. After 2 months, the rats were sacrificed, and the blood and liver samples of them were collected for biochemical and histopathological parameter analysis. All doses of T. graminifolius could significantly (p ≤ 0.05) decrease the raised levels of ALP, AST, ALT, GGT, cholesterol, LDL, triglyceride, total and conjugated bilirubin, and glucose and increased HDL, total protein, albumin, SOD, and CAT as compared to the untreated group. Also, aqueous extract of T. graminifolius decreased the degree of hepatic steatosis as compared to the untreated group. In conclusion, the obtained results demonstrated the hepatoprotective effect of T. graminifolius aqueous extract.

Keywords

Tragopogon graminifolius Aqueous extract Hepatoprotective effect Fatty liver High-fat diet 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethic approval

All institutional and national guidelines for the care and use of laboratory animals were followed.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Clinical Science, Faculty of Veterinary MedicineRazi UniversityKermanshahIran
  2. 2.Biotechnology and Medicinal Plants Research CenterIlam University of Medical SciencesIlamIran

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