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Comparative Clinical Pathology

, Volume 28, Issue 1, pp 241–248 | Cite as

Effect of aqueous ethanol extract of Dialium guineense leaf on diclofenac-induced oxidative stress and hepatorenal injuries in Wistar rats

  • Raphael J. OgbeEmail author
  • Carrol D. Luka
  • Godwin I. Adoga
Original Article
First Online:
  • 42 Downloads

Abstract

Drug-induced liver and kidney injuries are threats to public health worldwide, but there are claims that medicinal plants may provide remedy. Protective effect of Dialium guineense leaf extract (DGE) against diclofenac (DF)-induced oxidative injuries to the liver and kidney was evaluated in rats. Extract was prepared by maceration of pulverized sample in a mixture of water and ethanol, filtration, and evaporation to dryness. Eighteen rats were divided into three groups with six rats per group. Group I rats received normal saline, group II rats received 10 mg/kg DF by intramuscular route for 7 days while group III rats were treated with 250 mg/kg DGE orally for 28 days and given DF as group II rats. At the end of treatment, blood was collected from each rat and the serum was separated and used for spectrophotometric estimations of biomarkers of hepatorenal injuries. Liver and kidneys were excised from euthanized rats, homogenized in phosphate buffer and the supernatants used for assays of biomarkers of oxidative injuries. There was a significant (p < 0.01) increase in levels of ALT, AST, GGT, MDA, creatinine, and urea, and significant (p < 0.01) decrease in SOD, CAT, GPx, GST, GSH, and G6Pase of DF-intoxicated rats when compared with normal control. Pretreatment of DF-intoxicated rats with DGE significantly (p < 0.01) reduced the levels of ALT, AST, GGT, MDA, creatinine, and urea, and significantly (p < 0.01) elevated the levels of SOD, CAT, GPx, GST, GSH, and G6Pase compared with DF control. These findings showed that DGE may protect against DF-induced oxidative stress and hepatorenal injuries in rats.

Keywords

Antioxidants Free radicals Hepatotoxicity Lipid peroxidation Nephrotoxicity 
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Notes

Acknowledgements

The authors expressed their gratitude to Mr. Nathaniel Nyam of Nobel Diagnostic Laboratories Ltd., Jos, and Mr. Peter Akogwu of the Information and Communications Technology, Benue State University, Makurdi, Nigeria for their technical assistances.

Funding information

This work was supported by the Tertiary Education Trust Fund (TETFUND) grant, through the Federal University of Agriculture, Makurdi, Nigeria.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed. All procedures performed in the studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of Medical SciencesUniversity of JosJosNigeria
  2. 2.Department of Physiology, Pharmacology and Biochemistry, College of Veterinary MedicineFederal University of AgricultureMakurdiNigeria

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