Variations of immunoglobulin A in serum, tracheal, and intestinal tissues in chickens following inoculation of two strains of Newcastle disease vaccines
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To elucidate secretory immunoglobulin A (IgA) response as a part of mucosal immunity, it is decided to compare the effectiveness of intraocular administration of Hitchner B1 and thermostable I-2 vaccines in broiler chickens challenged with viscerotropic velogenic Newcastle disease virus (vvNDV) (Herts33). For this purpose, 301-day-old chicks were randomly divided in to the four groups. Groups 1 and 2 were inoculated with I-2 and HB1 vaccines twice via intraocular route with 1-week intervals, and groups 3 and 4 were considered positive and negative controls and birds were challenged 2 weeks after the second inoculation. Protection rate in I-2 and HB1 vaccine groups was 100% and 97% respectively with no significant differences (P > 0.05). All vaccinated groups showed elevation in ND HI antibody titer after vaccination. There were no significant differences (P > 0.05) in ND HI antibody between two vaccinated groups. First levels of detectable total IgA in a serum sample were observed 3 dpv1. No significant differences (P > 0.05) were observed between two vaccinated groups in IgA antibody titer in sera. The vaccinated groups were evaluated by IHC for detection IgA in the trachea and intestine. There were some differences between the vaccinated groups but these differences were not significant. Experimental studies have shown that both vaccines induce antibody production in mucosal surface and circulatory system and thermostable I-2 vaccine could be successfully used in the broiler chicken as effective as HB1 vaccine, so I-2 vaccine could be a suitable heat resistance candidate to reduce mortality in the poultry industry faced to vvND.
KeywordsImmunoglobulin A Newcastle disease Velogenic NDV Thermostable I-2 vaccine Hitchner B1 vaccine Broiler chicken
Days post vaccination
Days post challenge
Days post first vaccination
Days post second vaccination
This research was financially supported by a grant of Shiraz University Research Council.
Compliance with ethical standards
All experiments were conducted after institutional approval of the animal use committee of Shiraz University that follows the recommendations stated in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health.
Conflict of interests
The authors declare that they have no conflict of interest.
- Alders R, Spradbrow PB (2001) Controlling Newcastle disease in village chickens: a field manual. ACIAR Monograph 82. 112ppGoogle Scholar
- Al-Garib SO, Gielkens ALJ, Gruys E, Hartog L, Koch G (2003) Immunoglobulin class distribution of systemic and mucosal antibody responses to Newcastle disease in chickens. Avian Dis 47:32–40. https://doi.org/10.1637/0005-2086(2003)047[0032:ICDOSA]2.0.CO;2Google Scholar
- Habibi H, Nili H, Asasi K, Nazifi S, Mosleh N, Firouzi S (2013) Acute phase responses in village chicken challenged by a wild Newcastle disease virus isolate (JF820294.1.). Online J Vet Res 17:571–577Google Scholar
- Habibi H, Nili H, Asasi K, Mosleh N, Firouzi S, Mohammadi M (2015) Efficacy and transmissibility of Newcastle disease I-2 vaccine strain against a field isolate of virulent ND virus (JF820294.1) in village chicken. Trop Anim Health Prod 47(1):73–78. https://doi.org/10.1007/s11250-014-0687-1
- Hassanzadeh M, BozorgmehriFard MH (2004) A serological study of Newcastle disease in pre- and post-vaccinated village chickens in north of Iran. Int J Poult Sci 5:70–75Google Scholar
- Hsiang-jung TSA, Dih-Fa LIN (1998) Evaluation of the protection efficacy of newcastle disease vaccination programs. Journal of the Republic of China Veterinary Society, 1999Google Scholar
- Mahy BWJ, Kangro H (1996) Hemagglutination-inhibition. In: Virology methods manual. Academic Press Ltd., London, pp 114–116Google Scholar
- Quan S, Yunlian S, Ruiping S, Taozhen J, Decheng W, Ye D, Jun Y (2013) Detection of intestinal intraepithelial lymphocytes, goblet cells and secretory IgA in the intestinal mucosa during Newcastle disease virus infection. Avian Pathol 42:541–545. https://doi.org/10.1080/03079457.2013.845292 CrossRefGoogle Scholar
- Rauw F, Gardin Y, Palya V, Van Borm S, Gonze M, Lemaire S, van den Berg T, Lambrecht B (2009) Humoral, cell-mediated and mucosal immunity induced by oculo-nasal vaccination of one-day-old SPF and conventional layer chicks with two different live Newcastle disease vaccines. Vaccine 27:3631–3642. https://doi.org/10.1016/j.vaccine.2009.03.068 CrossRefGoogle Scholar
- Yan Z, Du Y, Zhao Q, Fan Q, Guo W, Ma R, Wang X, Zhu Z (2011) Mucosal immune responses against live Newcastle disease vaccine in immunosuppressed chickens. Pak Vet J 31:280–286Google Scholar