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Comparative Clinical Pathology

, Volume 28, Issue 1, pp 157–164 | Cite as

Anti-inflammatory and antioxidant activity of Thai mango (Mangifera indica Linn.) leaf extracts

  • Taddaow Khumpook
  • Supap Saenphet
  • Yingmanee Tragoolpua
  • Kanokporn SaenphetEmail author
Original Article
  • 29 Downloads

Abstract

Mango (Mangifera indica Linn.) has been widely studied as a folk medicines and it has become a common ingredient in cosmeceutical products. However, there was no report on the efficacy of Thai mango leaf extracts against inflammation. Thus, the aim of this study was to ascertain the anti-inflammatory and antioxidant activities of methanol (MeOH) and aqueous (Aq) extracts from the leaves of three mango varieties: Keaw Morakot, Nam Doc Mai, and Mahajanaka. To assay in vitro anti-inflammatory activity, the amount of nitric oxide (NO) released from macrophage cells was indirectly measured. Since free radicals play an important role in the inflammatory process, the antioxidant activity of M. indica leaf extracts was measured by ABTS and DPPH radical scavenging, ascorbic acid, and total phenolic assays. Mangiferin was also analyzed by HPLC. The most effective extract was selected and tested for anti-inflammatory activity in an animal model, an acute ear edema test in albino rats. The MeOH extract of Mahajanaka (M-MeOH) reduced nitric oxide production by LPS-stimulated macrophage cell lines RAW 264.7. M-MeOH showed the strongest antioxidant activity and it was, therefore, selected for an in vivo anti-inflammatory assay. An acute ear edema test in albino rats showed that M-MeOH exhibited significant anti-inflammatory activity compared to the control; ethyl phenylpropiolate-induced ear edema (P < 0.05). Moreover, mangiferin, a bioactive compound in M. indica leaves was detected in MeOH and Aq extracts. We concluded that the MeOH extract of M. indica leaves exhibits an anti-inflammatory property, both in vitro and in vivo, due to the presence of efficacious antioxidants.

Keywords

Mangifera indica Linn. Anti-inflammation Antioxidant Nitric oxide Ear edema Mangiferin 

Notes

Acknowledgements

We would like to thank the Science Achievement Scholarship of Thailand, the Department of Biology in the Faculty of Science at Chiang Mai University and the graduate school, Chiang Mai University and Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Faculty of Science, Chiang Mai University.

Funding information

This study was funded by the Science Achievement Scholarship of Thailand, the graduate school, Chiang Mai University.

Compliance with ethical standard

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures encompassing the animals were conducted with strict adherence to guidelines and procedures reviewed and approved by the Institutional Animal Care and Use Committee of the Biology Department, Faculty of Science, Chiang Mai University, permission number Re. 004/013.

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Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Doctor of Philosophy Program in Biology, Faculty of ScienceChiang Mai UniversityChiang MaiThailand
  2. 2.Department of Biology, Faculty of ScienceChiang Mai UniversityChiang MaiThailand

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