Hematological, biochemical, serological, and molecular monitoring of blood donor dogs vaccinated with CaniLeish® for the prevention of Leishmaniosis

  • Viola Vannucci
  • Alessandra Gavazza
  • Anyela Andrea Medina Valentin
  • Gori Veronica 
  • George Lubas
Original Article
  • 16 Downloads

Abstract

The use of systemic preventive measures in dogs living in areas with Leishmania infection is advisable, particularly in blood donors. The aim of this study was to monitor healthy blood-donor dogs immunized with the vaccine CaniLeish® by means of hematological, biochemical (serum total protein, albumin, protein electrophoresis, and C-reactive protein), serological (Speed Leish K™ and IFAT), and molecular assays (qualitative PCR, qlPCR). Twenty-four dogs were monitored at (T0), second (T1), third (T2) immunizations, and 2 months (T3) after the initial immunization and at the first (T4), second (T5), and third (T6) annual booster. The results were as follows: slight hyperproteinemia (29.5%, p > 0.05) observed throughout the monitoring period (T0-T6); increase in C-reactive protein (46.4%, p < 0.05) (at T3-T6); significant alterations in β-1 and β-2 globulin fractions (as absolute and percentage values, p < 0.05), Speed Leish K™ assay negative at T0 and T4-T6, positive IFAT titres of 1/40 (9.5%) at T0, and T3-T6, 1/80 (8.6%) at T1-T4, 1/160 (6.7%) at T1-T2 and T4, and 1/320 (1.9%) at T2 and T4; and qlPCR positive in 7.7% of samples at T3-T6. The vaccination with Canileish® induced changes in a few clinico-pathological and serological markers, which are likely associated with the activation of the immune response. These changes should be carefully considered when evaluating vaccinated dogs included in blood transfusion programs. The use of the CaniLeish® vaccine could be recommended as an additional preventive measure and could represent an important practice in the field of canine transfusion medicine.

Keywords

CaniLeish® Blood donor-dogs PCR Serology Leishmaniosis Vector-borne diseases 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

References

  1. Abdolali M, Mohebali M, Gholamhossein E, Zabih Z, Behnaz A, Bahram K, Shahram J, Mahmood M (2009) Canine visceral leishmaniasis: asymptomatic infected dogs as a source of L. infantum infection. Acta Trop 112:101–105CrossRefGoogle Scholar
  2. Belinchón-Lorenzo S, Iniesta V, Parejo JC, Fernández-Cotrina J, Muñoz-Madrid R, Soto M, Alonso C, Gomez-Nieto LC (2012) Detection of Leishmania infantum kinetoplast minicircle DNA by real time PCR in hair of dogs with leishmaniosis. Vet Parasitol 192:43–59CrossRefPubMedGoogle Scholar
  3. Bongiorno G, Paparcone R, Foglia Manzillo V, Oliva G, Cuisinier AM, Gradoni L (2013) Vaccination with LiESP/QA-21 (CaniLeish®) reduces the intensity of infection in Phlebotomus perniciosus fed on Leishmania infantum infected dogs—a preliminary xenodiagnosis study. Vet Parasitol 197:691–695CrossRefPubMedGoogle Scholar
  4. Ciaramella P, Corona M (2003) Canine Leishmaniasis: clinical and diagnostic aspects. Coompendiun on Continuing Education for the Practising. Vet N Am Ed 25(5):358–369Google Scholar
  5. Ciaramella P, Oliva G, De Luna R (1997) A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum. Vet Rec 141:539–543CrossRefPubMedGoogle Scholar
  6. De Freitas E, Melo M, Pimenta A, Marques M (2006) Transmission of Leishmania infantum via blood transfusion in dogs: potential for infection and importance of clinical factors. Vet Parasitol 137:159–167CrossRefPubMedGoogle Scholar
  7. EFSA AHAW Panel (EFSA Panel on Animal Health and Welfare) (2015) Scientific opinion on canine leishmaniosis. Eur Food Saf Authority J 13(4):1–77Google Scholar
  8. El Tai NO, Osman OF, El Fari M, Presber W, Schönian G (2000) Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg 94(5):575–579CrossRefPubMedGoogle Scholar
  9. EMA (European Medicines Agency) (2011) Scientific discussion. EMA/CVMP/296057/2010. Veterinary medicines and product data managementGoogle Scholar
  10. Ferroglio E, Zanet S, Mignone W, Poggi M, Trisciuoglio A, Bianciardi P (2013) Evaluation of a rapid device for serological diagnosis of Leishmania infantum infection in dogs as an alternative to immunofluorescence assay and western blotting. Clin Vaccine Immunol 20(5):657–659CrossRefPubMedPubMedCentralGoogle Scholar
  11. Jain K, Jain NK (2015) Vaccines for visceral leishmaniasis: a review. J Immunol Methods 422:1–12CrossRefPubMedGoogle Scholar
  12. Mancianti F, Meciani N (1988) Specific serodiagnosis of canine leishmaniasis by indirect immunofluorescence, indirect hemagglutination, and counterimmunoelectrophoresis. Am J Vet Res 49(8):1409–1411PubMedGoogle Scholar
  13. Maroli M, Gradoni L, Oliva G, Castagnaro M, Crotti A, Lubas G, Paltrinieri S, Roura X, Zini E, Zatelli A (2010) Guidelines for prevention of leishmaniasis in dogs. J Am Vet Med Assoc 236(11):1200–1206CrossRefPubMedGoogle Scholar
  14. Martin V, Vouldoukis I, Moreno J, McGahie D, Gueguen S, Cuisinier AM (2014) The protective immune response produced in dogs after primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) remains effective against an experimental challenge one year later. Vet Res 45(1):69CrossRefPubMedPubMedCentralGoogle Scholar
  15. Martinez-Subiela S, Tecles F (2002) Serum concentrations of acute phase proteins in dogs with leishmaniasis. Vet Rec 150:241–244CrossRefPubMedGoogle Scholar
  16. Ministero della Salute (Italian Ministry of Health) (2016) Linea guida per l’esercizio delle attività sanitarie riguardanti la medicina trasfusionale in campo veterinario (Guidelines concerning the practice of health procedures about veterinary transfusion medicine) Official Journal 25:5–18Google Scholar
  17. Moreno J, Vouldoukis I, Martin V, McGahie D, Cuisinier AM, Gueguen S (2012) Use of a LiESP/QA-21 vaccine (CaniLeish®) stimulates an appropriate Th1-dominated cell-mediated immune response in dogs. PLoS Negl Trop Dis 6(6):e1683CrossRefPubMedPubMedCentralGoogle Scholar
  18. Moreno J, Vouldoukis I, Schreiber P, Martin V, McGahie D, Gueguen S, Cuisinier AM (2014) Primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) produces a cell-mediated immune response which is still present 1 year later. Vet Immunol Immunopathol 158:199–207CrossRefPubMedGoogle Scholar
  19. MorenoVouldoukis J, Schreiber I, Martin P, McGahie V, Gueguen D, Cuisinier AM (2014) Primary vaccination with the LiESP/QA-21 vaccine (CaniLeish®) produces a cell-mediated immune response which is still present 1 year later. Vet Immunol Immunopathol 158:199–207CrossRefGoogle Scholar
  20. Noli C, Saridomichelakis MN (2014) An update on the diagnosis and treatment of canine leishmaniosis caused by Leishmania infantum (syn. L. chagasi). Vet J 202:425–435CrossRefPubMedGoogle Scholar
  21. Oliva G, Nieto J, Foglia Manzillo V, Cappiello S, Fiorentino E, Di Muccio T, Scalone A, Moreno J, Chicharro C, Carrillo E, Butaud T, Guegand L, Martin V, Cuisinier AM, McGahie D, Gueguen S, Canavate C, Gradoni L (2014) A randomised, double-blind, controlled efficacy trial of the LiESP/QA-21 vaccine in Naïve dogs exposed to two Leishmania infantum transmission seasons. PLoS Negl Trop Dis 8(10):e3213CrossRefPubMedPubMedCentralGoogle Scholar
  22. Owens SD, Oakley DA, Marryot OK, Hatchett W, Walton R, Nolan TL, Newton A, Steurer F, Schantz P, Giger U (2001) Transmission of visceral leishmaniosis through blood transfusion from infected English foxhounds to anemic dogs. J Am Vet Med Assoc 219:1076–1083CrossRefPubMedGoogle Scholar
  23. Paltrinieri S, Solano-Gallego L, Fondati A, Lubas G, Gradoni L, Castagnaro M, Zatelli A (2010) Guidelines for diagnosis and clinical classification of leishmaniasis in dogs. J Am Vet Med Assoc 236(11):1184–1191CrossRefPubMedGoogle Scholar
  24. Pennisi MG (2014) Leishmaniosis of companion animals in Europe: an update. Vet Parasitol 208:35–47CrossRefPubMedGoogle Scholar
  25. Rotondi S (2013) Diagnosi della Leishmaniosi canina: Speed Leish K e l’interesse delle kinesine. Virbacnews n.1-Speciale leishmaniosiGoogle Scholar
  26. Sagols E, Martin V, Claret E, McGahie D, Ciusinier AM, Gueguen S (2012) Evaluation of the humoral immune response after vaccination with LiESP/QA- 21 (CaniLeish): interest of Leishmania specific anti-kinesin antibodies detection. In Proceedings of the WSAVA/FECAVA/BSAVA CongressGoogle Scholar
  27. Sagols E, Ferraz F, Claret E, McGahie D (2013) Follow up of the humoral immune response after the first annual CaniLeish® booster vaccination. In Proceedings of the Southern European Veterinary Conference (SEVC’13)Google Scholar
  28. Solano-Gallego L, Miró G, Koutinas A, Cardoso L, Pennisi MG, Ferrer L, Bourdeau P, Oliva G, Baneth G (2011) Leishvet guidelines for the practical management of canine leishmaniosis. Parasit Vectors 4:86CrossRefPubMedPubMedCentralGoogle Scholar
  29. Solano-Gallego L, Villanueva-Saz S, Carbonell M, Trotta M, Furlanello T, Natale A (2014) Serological diagnosis of canine leishamniosis: comparison of three commercial ELISA tests (Leiscan®, ID screen® and Leishmania 96®), a rapid test (Speed Leish K™) and an in-house IFAT. Parasites Vectors 7(111):10Google Scholar
  30. Solano-Gallego L, Cardoso L, Pennisi M-G, Petersen C, Bourdeau P, Oliva G, Miró G, Ll F, Baneth G (2017) Diagnostic challenges in the era of canine Leishmania infantum vaccines. Trends Parasitol 33(9):706–717CrossRefPubMedGoogle Scholar
  31. Starita C, Gavazza A, Lubas G (2016) Hematological, biochemical, and serological findings in healthy canine blood donors after the administration of CaniLeish® Vaccine. Vet Med Int 4601893:6Google Scholar
  32. Tabar MD, Roura X, Francino O, Altet L, Ruiz de Gopegui R (2008) Detection of Leishmania infantum by real-time PCR in a canine blood bank. J Small Anim Pract 49:325–328CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag London Ltd., part of Springer Nature 2018

Authors and Affiliations

  • Viola Vannucci
    • 1
  • Alessandra Gavazza
    • 1
  • Anyela Andrea Medina Valentin
    • 1
  • Gori Veronica 
    • 1
  • George Lubas
    • 1
  1. 1.Veterinary Transfusional Center (CTV), Department of Veterinary SciencesUniversity of PisaPisaItaly

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