Prognostic value of zymographic gelatinase activity of MMP-2 and MMP-9 in tumor recurrence of canine intrascrotal hemangiomas

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There is limited information about intrascrotal hemangioma tumors and their gelatinase activity in the dogs. This study was planned to identify the serum gelatinases in male dogs affected by intrascrotal hemangioma and determine their prognostic value in tumor recurrence. Ten dogs with intrascrotal hemangioma were diagnosed among a total of 65 testicular excision samples. According to the incidence of recurrence, the patients divided into two groups: tumors with recurrence and tumors with no-recurrence. Serum gelatinase activity was assayed by semi-quantitative zymography to determinate their prognostic value in canine intrascrotal hemangioma. Both latent and active MMP-9 and only latent MMP-2 appeared in the gels. Gelatinases showed a significant higher serum activity in dogs with hemangioma than those of the normal dogs. There was a significant association between increased serum activity of gelatinase A and gelatinase B and tumor recurrence. The dogs with hemangioma had a shorter disease-free survival time; however, multivariate analysis showed that serum activity of MMP-2 and MMP-9 was not an independent prognostic factor. According to the findings, MMP-2 and MMP-9 may cause a progressive angiogenesis in canine intrascrotal hemangioma and tumor recurrence subsequently. Our results showed that serum activity of MMP-2 and MMP-9 may be used as a non-invasive prognostic marker for tumor recurrence prediction in dogs affected by intrascrotal hemangioma.

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We thank the staffs of the Dr. Rastegar’s laboratory and Paytakht Pet Hospital.


This study was funded by Research Deputy of University of Tehran (approximately 900 $).

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Correspondence to Zohreh Khaki.

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The authors declare that they have no conflict of interest.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study. Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.

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Fathipour, V., Khaki, Z., Nassiri, S.M. et al. Prognostic value of zymographic gelatinase activity of MMP-2 and MMP-9 in tumor recurrence of canine intrascrotal hemangiomas. Comp Clin Pathol 29, 25–31 (2020) doi:10.1007/s00580-018-2677-6

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  • Zymography
  • Serum gelatinase
  • Canine intrascrotal hemangioma
  • Recurrence