Comparative Clinical Pathology

, Volume 28, Issue 1, pp 3–10 | Cite as

Applications of fluorescence in situ hybridization in detection of disease biomarkers and personalized medicine

  • Farzaneh Bozorg-GhalatiEmail author
  • Iraj Mohammadpour
  • Reza Ranjbaran
Review Article


Fluorescence in situ hybridization (FISH) method, as a molecular technique, is applicable for studying the gene expression during the cell differentiation, capturing images from the chromosomes/chromatin’s areas in interphase, and detecting the chromosomal abnormality and rearrangements. This potential and impressive technique with high sensitivity and specificity could detect the biomarkers of various diseases. Therefore, it is very useful for accelerating therapy and enhancing the prognosis of the disease. A glimpse at this molecular technique and focus on its effective applications confirm it as a supreme tool for clinical diagnosis and principles of personalized medicine.


Fluorescence in situ hybridization Biomarker Genetic aberrations Infectious diseases Gastrointestinal diseases Cancers 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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© Springer-Verlag London Ltd., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Molecular Pathology, Nemazee Hospital, School of MedicineShiraz University of Medical SciencesShirazIran
  2. 2.Department of Medical Parasitology and Mycology, School of MedicineShiraz University of Medical SciencesShirazIran
  3. 3.Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical SciencesShiraz University of Medical SciencesShirazIran

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