Haematopoietic induction and hepatic protective roles of Hepacare® in CCl4-induced hepatic damaged rats
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Herbal formulations are plant parts used as raw materials for self-administered pharmaceutical remedies, and many of them are being sold without any scientific validation for their potency and efficacy. This research work was aimed at evaluating the haematopoietic, biochemical, and histological effects of Hepacare®, a popularly sold herbal formulation in Nigeria against carbon tetrachloride (CCl4)-mediated liver damage in rats. Haematological analysis showed significant reduction (p < 0.05) in haemoglobin, red blood cell, packed cell volume, and platelet counts in CCl4-treated group when compared with the untreated group. These parameters were however reversed across the groups treated with the herbal formulation. Levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin were significantly (p < 0.05) reduced after treatment of rats with the formulation which were previously elevated (p < 0.05) in the CCl4-treated group when compared with the untreated group. The CCl4-treated group exhibited significantly different activities in liver SOD and GSH enzymes. The level of MDA was lowered in the liver tissue samples of treated rats when compared with the CCl4-exposed untreated rats. The groups treated with the formulation showed signs of protection against this toxicant as evidenced by the absence of necrosis. Hepacare® showed reversal effects on the previously increased haematological parameters and damaged liver tissues with a potential to ameliorate oxidative stress in hepatic dysfunction.
KeywordsHepacare® Carbon tetrachloride Haematologic Liver injury Hepatic marker enzymes Antioxidant markers Histological examination
This research was supported by Covenant University, Canaan Land, Ota, Nigeria, through a seed grant (CUCERD-2014) given to AHA. The support from the technical staff of the Biochemistry Unit, Department of Biological Sciences, Covenant University, is greatly appreciated.
Compliance with ethical standards
The research was approved by the Department of Biological Sciences Research Ethics Committee, Covenant University. All animals were also treated in line with the National Institutes of Health (NIH) guidelines for the use and care of animals in the laboratory (National Institute of Health (NIH) 2011).
Conflict of interest
The authors declare that they have no conflict of interest.
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